Method for concentrating and desalting urine sample
A urine and sample technology, applied in the field of biological detection, can solve the problems of inability to effectively concentrate the original sample, poor desalination effect, protein degradation, etc., and achieve the effects of reduced salt concentration, increased process, and more protein loss
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[0029] The present invention will be further described below in conjunction with specific examples, but it should not be construed as a limitation of the present invention.
[0030] Take the processing of the urine sample of IgA nephropathy patient as example to illustrate the present invention:
[0031] 1. Collect 20ml of mid-section urine from the patient and freeze it at -80°C for later use;
[0032] 2. Add 3uL 200mM phenylmethylsulfonyl fluoride (PMSF) to every 10ml of urine to prevent proteolysis;
[0033] 3. Divide the above 20ml mixed urine into two tubes and centrifuge at 15000g for 5-10 minutes at 2°C-4°C;
[0034] 4. Collect the supernatant and filter it through a 0.45u nylon filter to remove cell nuclei and bacteria;
[0035] 5. The filtered urine is further concentrated and desalinated by cold acetone or trichloroacetic acid (TCA), centrifuged at 3000g for 10-30 minutes at 2°C-4°C, and the precipitate is taken and dried in the air for 5-10 minutes ;
[0036] 6....
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