Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for synthesizing human milk fucosylation oligosaccharide by using genetic engineering strain through coupling and fermenting

A technology of genetically engineered strains and fucoida hominis, applied in the field of genetic engineering, can solve the problems of complex synthesis routes, expensive glycoside donors, inability to achieve large-scale production, etc., to reduce production costs, strengthen basic theoretical research value and social economy. Benefit, the effect of broad market development prospects

Inactive Publication Date: 2011-07-13
TIANJIN UNIV OF SCI & TECH
View PDF4 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Human milk oligosaccharides are obtained by chemical synthesis. Due to the complexity of the synthetic route and the high cost of glycoside donors, most processes still cannot achieve large-scale production, which has become an insurmountable obstacle for chemical synthesis of oligosaccharides.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for synthesizing human milk fucosylation oligosaccharide by using genetic engineering strain through coupling and fermenting
  • Method for synthesizing human milk fucosylation oligosaccharide by using genetic engineering strain through coupling and fermenting
  • Method for synthesizing human milk fucosylation oligosaccharide by using genetic engineering strain through coupling and fermenting

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0031] Below in conjunction with the examples, the present invention is further described, the following examples are illustrative, not limiting, and the protection scope of the present invention cannot be limited by the following examples.

[0032] The method for constructing human lactofucosylated oligosaccharide system by coupling fermentation with genetic engineering strains involved in the present invention is to use the E. coli expression system BL21 / pET-22b to catalyze the synthesis of human milk rock with mannose as a substrate Cocosylated oligosaccharide-related enzyme genes such as hexokinase gene, phosphomannose mutase gene, mannose-1-phosphate guanylyltransferase gene, GDP-mannose 4,6-dehydratase, GDP -4-keto-6-deoxymannose 3,5-mutrotase / 4-reductase and α-1,2 fucosyltransferase genes (glk, manB, manC, gmd, wcaG, fuct) for efficient Expression and construction of three recombinant E. coli strains containing different target genes, and efficient synthesis of human mi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for synthesizing human milk fucosylation oligosaccharide by using a genetic engineering strain through coupling and fermenting. A metabolic engineering strain is constructed by mainly using a genetic engineering technology so that efficient expression of a key enzyme in a metabolic pathway is realized and biosynthesis of the human milk fucosylation oligosaccharide is realized in vitro. Due to the adoption of the engineering strain constructed by using the method, the copy number of enzyme genes can be increased, and the expression quantity of the enzyme can be improved, therefore, the human milk fucosylation oligosaccharide can be efficiently expressed. The invention provides a feasible path for industrially synthesizing the human milk oligosaccharide at low cost, and has stronger fundamental theoretical research value, larger economic and social benefits and wide market development prospect.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and relates to the construction technology of genetic engineering strains, in particular to a method for synthesizing human milk fucosylated oligosaccharides by coupled fermentation using genetic engineering strains. Background technique [0002] In recent years, with the rapid development of food industry and biotechnology, the development of functional oligosaccharides has become an important topic in the field of international biotechnology. industry. Human milk oligosaccharides not only have physiological effects such as anti-viral infection, enhancing immune regulation, and reducing inflammation, but also have certain preventive effects on cancer and chronic recurrent colitis. As a class of carbohydrates with a special structure, it can act as a soluble receptor analog of epithelial cells and participate in the enhancement of the non-immune defense system of breastfed infants. [0003] T...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/09C12P19/00
Inventor 李玉路福平贾红红王春霞王洪彬刘逸寒
Owner TIANJIN UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com