Preparation method of quantitative microorganism freeze-dried product

Abstract

The invention discloses a preparation method of a quantitative microorganism freeze-dried product. The preparation method comprises the following steps of: diluting microorganisms at a stable period to obtain uniform diluent and preparing the diluent into bacterial suspension with quantitative concentration; mixing the bacterial suspension with a protecting agent to obtain mixed liquor; and bottling quantitative mixed liquor, pre-freezing at the temperature of -30 DEG C, freezing and drying in vacuum, and sealing in vacuum to obtain the quantitative microorganism freeze-dried product, wherein the protecting agent comprises the following components in parts by weight: 0.1-10 parts of water-soluble sugar, 0.1-5 parts of defatted milk powder, 0.1-5 parts of antioxidant, 0.1-20 parts of gelatin and 0-10 parts of active carbon. The microorganism freeze-dried product prepared by adopting the preparation method contains basically quantitative microorganisms, and is convenient to use; and the activity of the microorganisms can be ensured when the product is stored at the temperature of below 8 DEG C, and the biological and chemical properties of the microorganisms do not change basically within the retention period.

Description

technical field [0001] The invention relates to a method for preparing a freeze-dried product of microorganisms, in particular to a method for preparing a quantitative freeze-dried product of microorganisms. Background technique [0002] Strains are an important biological resource, and strain preservation is an important basic work of microorganisms. The international community has always paid more attention to the preservation, sharing and exchange of microbial strain resources. As early as the end of the 19th century, Europe began to have some well-managed culture preservation institutions; in the early 20th century, some famous culture preservation institutions were established one after another. In 1904, NRRL was established in the United States, and in 1925, ATCC was established. At present, ATCC has developed Become the world's largest culture preservation and supply center. [0003] The preservation of quality control strains has been studied abroad, and its princip...

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Problems solved by technology

[0006] So far, domestic strains have been in the form of relatively simple freeze-dried tubes, and there has been no research and development of strains with a certain amount of bacteria

In the process of opening the freeze-drying tube for resuscitation, a little carelessness may burst the freeze-drying tube, which is not only easier to contaminate the target bacteria, but also easier to burn or cut yourself, and it is easier to form aerosols during the operation, which is harmful to people. threat to the health of

[0007] At present, domestic grass-roots units and most production enterprises generally do not have strain management specialists, the preservation facilities are not complete, and the professional operation level of microbiological inspection personnel is not high, so it is relatively easy to cause death, Variation, contamination, etc.

Moreover, the preserved strains often need to go through cumbersome processes such as resuscitation, transfer, and dilution during use, which not only consumes time and a lot of manpower and material resources, but also may affect the accuracy and reliability of the test results, seriously reducing work. Efficiency, so that the quality of the product cannot be guaranteed. If the experiment fails due to the strain problem, re-sampling is required, which delays the sales of the product and increases the cost of the enterprise.

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