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Method for producing liquid cellulose by mixed fermentation of microbe

A technology of mixed fermentation and cellulase, which is applied in the field of microbial engineering, can solve the problems of little increase in activity of β-glucosidase, lack of activity of β-glucosidase, incomplete composition of cellulase enzyme system, etc., to achieve perfect enzyme System components, no environmental pollution, the effect of improving vitality

Inactive Publication Date: 2011-08-17
河南天冠纤维乙醇有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the following problems are often encountered in production: low enzyme activity; incomplete composition of the cellulase enzyme system produced by Trichoderma, lack of β-glucosidase or extremely low activity
It has been reported in the literature that the mixed fermentation of Trichoderma viride and Rhizopus produced cellulase, and the enzyme activity was greatly improved, but the activity of β-glucosidase was not greatly improved.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 Incline strains, preparation of secondary liquid seeds

[0047] ⑴Production strain Trichoderma viride ( Trichoderma viride ) and Aspergillus niger ( Aspergillus niger ).

[0048] (2) Preparation of slant strains Prepare PDA medium, use streaks to inoculate Trichoderma viride and Aspergillus niger strains, cultivate at 30°C for 7 and 5 days respectively, and form a large number of spores, which are used as seed liquid inoculation.

[0049] ⑶Preparation of secondary liquid bacteria with bran 4%, (NH4) 2 SO 4 0.4%, KH 2 PO 4 0.6%, CaCl 2 0.05%, MgSO 4 ·7H 2 O 0.08%, the rest is a liquid medium of water, bottled and sterilized, and the spore suspension is inoculated according to the inoculum amount of 4-6%. Cultivate at 30°C, 220rpm for 48h.

Embodiment 2

[0050] Example 2 Preparation of liquid fermentation medium

[0051] Make a mixed slurry with 4.0% microcrystalline cellulose, 2.0% corn cob, 0.8% corn steep liquor, 80% water, and 0.2% nutrient salt, adjust the pH between 4.5 and 5.0, and sterilize by high-pressure steam at 121°C for 30 minutes .

Embodiment 3

[0052] Example 3 Feed medium

[0053] 1.0% microcrystalline cellulose, 3.0% corn cob, 2.0% corn steep liquor, 80% water, 0.1% nutrient salt, make a mixed slurry, adjust the pH between 5.0 and 6.0, and sterilize by high-pressure steam at 121°C for 30 minutes.

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PUM

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Abstract

The invention relates to a method for producing liquid cellulose by the mixed fermentation of microbe. The method comprises the following steps: producing bacterium by taking trichoderma viride and aspergillus niger as cellulose; vaccinating spore liquid or seed liquid in a liquid culture medium; and producing high-activity cellulose by controlling the constituent of the culture medium, the growth amount of two bacterium during cultivation by means of mixed fermentation, the cultivation condition and the cultivation mode. The method has good effects for improving the vigor of the cellulose, perfecting the constituent of the enzyme system and shortening the fermentation time, is simple and easy to implement, is very wide in development prospect, and is environment-friendly when the natural cellulose resources are developed and used such as the straw and other natural cellulose, thereby being an effective method for promoting the industrialization of the straw alcohol.

Description

technical field [0001] The invention belongs to the technical field of microbial engineering, and in particular relates to a method for producing liquid cellulase by microbial mixed fermentation. Background technique [0002] Cellulase refers to the general term for a group of enzymes that can hydrolyze cellulose β-1,4 glucosidic bonds and turn cellulose into cellobiose and glucose. It is not a single enzyme, but a multi-component enzyme that acts synergistically It is widely used in many fields of industry and agriculture. For example, cellulase can bioconvert industrial and agricultural waste such as crop stalks and bagasse into glucose, which can be further converted into biochemical products such as alcohol and single-cell protein by other microorganisms. As a demulsifier, it can maintain underground fractures and maintain underground pressure in oil extraction, and has no pollution to the formation and the environment. Using cellulase as a feed additive can improve th...

Claims

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Application Information

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IPC IPC(8): C12N9/42C12R1/885C12R1/685
Inventor 王林风赵子高吴金辉闫德冉
Owner 河南天冠纤维乙醇有限公司