Method for searching heavy metal hyper accumulator

A technology for hyper-accumulating plants and heavy metals, applied in the field of finding hyper-accumulating plants for heavy metals, can solve the problems of ignoring plants with repair potential and limited plant species, and achieve the effect of simple and easy operation and rich plant species

Inactive Publication Date: 2011-08-24
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Application Information

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Problems solved by technology

However, both methods have a disadvantage that cannot be ignored, that is, the limitation of p

Method used

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  • Method for searching heavy metal hyper accumulator
  • Method for searching heavy metal hyper accumulator
  • Method for searching heavy metal hyper accumulator

Examples

Experimental program
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Effect test

Embodiment 1

[0017] Example 1: Soil Seed Bank - Preliminary Screening Experiment of Heavy Metal Enrichment Plants

[0018] The experiment was carried out in the greenhouse of South China Botanical Garden in Guangzhou. Garden soil was picked from the botanical garden, dried in the sun, passed through a 1 cm sieve, and filled into pots (￠=30 cm, H=28 cm), each pot was filled with 12.5 kg of soil, and CdCl was added to the soil 2 , ZnSO 4 7H2O, Pb(NO 3 ) 2 、CuSO 4 ·5H 2 O solution, the concentration is set as: Cd: 4 mg / kg, 8 mg / kg, Zn: 100 mg / kg, Pb: 300 mg / kg, 600 mg / kg, Cu: 100 mg / kg, 300 mg / kg, The treatment without adding heavy metals was used as the control, with a total of 8 treatments without repetition. Since there are plant seeds in the recovered soil, there is no need to sow or transplant seedlings in addition. 65 days after adding the heavy metal solution, identify and record the plant species in the pot ( figure 1 ), among all the plants, it was found that Solanum nigrum p...

Embodiment 2

[0022] Embodiment Two: Soil Seed Bank—Cd Concentration Gradient Test

[0023] Through the above-mentioned preliminary experiment (Example 1), it was found that Solanum nigrum has strong tolerance to Cd, and can enrich a higher content of Cd ( figure 2 ). In order to further investigate the tolerance and absorption capacity of Solanum nigrum to Cd, a Cd concentration gradient experiment was designed to test its tolerance and enrichment characteristics to Cd.

[0024] The experiment was carried out in the greenhouse of South China Botanical Garden in Guangzhou. Use the same vegetable garden soil as the above screening experiment, put in pots (￠=16 cm, H=14 cm), 2 kg per pot. There is no need to sow or transplant seedlings since the seeds of the nightshade are already in the soil. Adding CdCl to the soil 2 solution, Cd concentrations were 0, 8, 15, 30, 60 and 100 mg / kg, respectively, a total of 6 treatments, repeated 4 times. Water according to the water needs of the plants...

Embodiment 3

[0028] Embodiment three: transplant seedlings-Cd concentration gradient test:

[0029] The experiment was carried out in the greenhouse of South China Botanical Garden in Guangzhou. Use the same vegetable garden soil as the above screening experiment, put in pots (￠=30 cm, H=28 cm), fill each pot with 12.5 kg of soil, and then add different concentrations of CdCl to the soil 2 solution. Add according to the soil Cd concentration of 0, 8, 15, 30, 60, 100 mg / kg, a total of 6 concentration treatments, repeated 4 times. After balancing for 1 month, pour out the soil in each pot and mix evenly, so that the heavy metals and nutrients in the soil are evenly distributed, and then put them into the pots. Transplant 5 cm high (after 10 days of seed germination) Solanum nigrum seedlings into pots, 3 strong seedlings per pot. During the growth period, the plants were watered according to the water demand of the plants, and the plants were harvested 60 days after transplanting the seedl...

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Abstract

The invention relates to a method for searching a heavy metal hyper accumulator, screening plants with hyper-accumulation characteristic to heavy metal by utilizing soil seeds and then verifying the hyper-accumulation characteristic of the plants by virtue of a heavy metal concentration gradient experiment. The method comprises the following steps of: acquiring soil rich in seed resources from the field and filling the soil into a pot; adding multiple heavy metal solution, waiting seeds in the soil to germinate, wherein the germinated seeds have high tolerance on a heavy metal; when the plants grow up, selecting the plants with high biomass liveweight and high heavy metal tolerance to determine the content of the heavy metal; and selecting the plants with strong accumulation ability to carry out the next heavy metal concentration gradient experiment. The method provided by the invention has the advantages that: 1, the varieties of the plants in a soil seed library are rich, and the accumulation characteristics of multiple plants to multiple heavy metals can be tested in one step; and 2, the soil seed libraries are located everywhere, the experiment is simple and easy to operate, and hyper accumulation plants can be conveniently searched in a global scale.

Description

technical field [0001] The invention relates to soil pollution remediation, that is, a method for finding heavy metal super-accumulating plants. Background technique [0002] Due to the development of industrial and mining enterprises, excessive use of agricultural chemical fertilizers, sewage irrigation, etc., soil heavy metal pollution in China and the world is becoming more and more serious. Phytoremediation technology is currently a research hotspot in the treatment of heavy metal pollution. It has the advantages of permanent treatment effect, in-situ treatment process, low treatment cost, environmental aesthetic compatibility and simplicity of post-processing. The key to the success of this technology is to find hyper-accumulator plants, such as the ability to enrich 1000 mg / kg of As, Pb, Cu, Ni and Co, 10000 mg / kg of Zn and Mn, and 100 mg / kg of Cd to the aerial parts of plants. Although more than 400 hyperaccumulator plants have been discovered in the world, most of t...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01G7/00
Inventor 夏汉平张杏锋李志安庄萍
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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