Method for realizing excellent plant body induction and successive propagation culture on dendrobium candidum by virtue of plant tissue culture

A technology of plant tissue culture and Dendrobium candidum, which is applied in the field of plant tissue culture, can solve the problems affecting the in-depth research and application of Dendrobium candidum, and achieve the effects of maintaining the efficacy of drugs, short cycle time and low production cost

Inactive Publication Date: 2011-08-24
FUJIAN GRASS KING BIOLOGICAL GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

An effective culture technique has not been found to solve the doubts and difficulties in the induction and pr

Method used

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  • Method for realizing excellent plant body induction and successive propagation culture on dendrobium candidum by virtue of plant tissue culture
  • Method for realizing excellent plant body induction and successive propagation culture on dendrobium candidum by virtue of plant tissue culture
  • Method for realizing excellent plant body induction and successive propagation culture on dendrobium candidum by virtue of plant tissue culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: a kind of method of producing dendrobium candidum by tissue culture, comprises the steps:

[0027] A. Plant selection and aseptic treatment:

[0028] A) select the wild dendrobium candidum plant that grows in October, rinse with tap water for 2-3 hours, clean with a hairbrush, remove roots and leaves, then rinse with distilled water, put in the clean workbench;

[0029] B) Soak the stem plant body from which roots and leaves have been removed with 70% alcohol for 30 seconds to sterilize the surface, and then clean it with sterile water;

[0030] C) Soak and sterilize the stem section plant body through alcohol sterilization with 0.3% sodium hypochlorite solution for 10 minutes, rinse 5 times with sterile water, then transfer to 0.1% mercuric chloride solution for sterilization for 8 minutes, pour off the sterilizing solution, Rinse 4-5 times with sterile water prepared in advance, and then dry the sterile water with filter paper;

[0031] D) under asepti...

Embodiment 2

[0036] Embodiment 2: a kind of method of producing dendrobium officinale by tissue culture, it comprises the steps:

[0037] a. Plant selection and aseptic treatment:

[0038] A.) Select the wild Dendrobium candidum plant grown in July, wash it with tap water for 2-3 hours, clean it with a hair brush, remove the roots and leaves, rinse it with distilled water, and put it in the clean workbench;

[0039] B) Soak the stem plant body from which roots and leaves have been removed for 25 seconds with 70% alcohol to sterilize the surface, and then clean it with sterile water;

[0040]C) Soak and sterilize the stem section plant body through alcohol sterilization with 0.6% sodium hypochlorite solution for 5 minutes, rinse 4 times with sterile water, then transfer to 0.2% mercuric chloride solution for sterilization for 4 minutes, pour off the sterilizing solution, Rinse 4-5 times with sterile water prepared in advance, and then dry the sterile water with filter paper;

[0041] D) u...

Embodiment 3

[0056] Embodiment 3: Adopt MS culture medium, improved MS culture medium to carry out the culture experiment of inducing bunchy buds to the Dendrobium candidum subsection tissue after the aseptic treatment respectively, this experiment is repeated three times, and culture condition is identical with embodiment 2, and the test investigates its iron skin Differentiation number, tissue fresh weight, differentiation rate and growth vigor of Dendrobium. The test results are as follows:

[0057] Experimental Comparison of Different Media and Different Concentrations of Hormone on Cluster Buds

[0058]

[0059] Note: + means the color is yellowish, ++ means the color is slightly yellow, +++ means better, ++++ means very green seedlings.

[0060] Test result proves, the present invention adopts two kinds of different basal culture medium and the induction culture medium that adds the auxin of different concentration to carry out to culture the Dendrobium candidum subsection after ...

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Abstract

The invention discloses a method for realizing excellent plant body induction and successive propagation culture on dendrobium candidum by virtue of plant tissue culture. The method comprises three links: selecting a plant and sterilizing, inducing and culturing cluster buds and carrying out propagation culture in a large scale; and the method is characterized in that two different culture mediums are adopted in the two stages of inducing and culturing as well as culturing in the large scale. The method disclosed by the invention is easier to operate, the production cost is low, the method is applicable to industrial seed culturing, the yield is high, the quality is good, no pollution is produced to the environment, and batch production can be realized.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, in particular to a method for plant tissue culture production, tissue culture production of Dendrobium officinale induction and subculture culture. Background technique [0002] Dendrobium officinalis (Herba Dendrodii Officinalis) is a perennial herb of Orchidaceae, an endangered and precious medicinal whole herb, and a traditional precious medicinal material in my country. Also known as Chlorophytum chinensis, Tiepilan, Lishucao. Wild Dendrobium candidum is mostly distributed in countries and regions such as East Asia, Southeast Asia and Australia. Our country is distributed in the mountainous areas of Zhejiang, Yunnan, Fujian and other places. It is mainly issued in the high mountains, cliffs and rock crevices in the above-mentioned areas. Due to the special growth conditions and distribution limitations of Dendrobium officinale, and after long-term excavation, the natural resou...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈咏梅黄碧华
Owner FUJIAN GRASS KING BIOLOGICAL GRP
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