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Detection kit and detection method for polymorphism of sheep BMPR-1B gene A746G

A technology of polymorphism detection and detection method, which is applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., and can solve the problems of slow detection speed and inability to adapt to the detection of target genes

Inactive Publication Date: 2011-08-24
XINJIANG ACADEMY OF AGRI & RECLAMATION SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, for the detection of BMPR-1B gene, most researchers use PCR-RFLP technology, but PCR-RFLP technology has a slow detection speed and cannot meet the requirements of large-scale and parallel detection of target genes in sheep populations

Method used

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  • Detection kit and detection method for polymorphism of sheep BMPR-1B gene A746G
  • Detection kit and detection method for polymorphism of sheep BMPR-1B gene A746G
  • Detection kit and detection method for polymorphism of sheep BMPR-1B gene A746G

Examples

Experimental program
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Embodiment 1

[0071] reference figure 1 - Figure 5 In the following, the technical solution of the present invention will be described in detail in the embodiments with reference to the accompanying drawings. The steps of this embodiment are as follows:

[0072] 1. Prepare multiple sheep chromosomal DNA for use

[0073] (1) Collection: This method is suitable for EDTA anticoagulated whole blood samples. Collect 0.5ml of the venous blood of the tested sheep, draw it with a disposable sterile injection needle, and collect it in a sterile 1.5ml centrifuge tube containing 100μl of 2% EDTA solution, cover the tube, and invert it several times to mix.

[0074] (2) Storage: Blood samples should be stored at 2-8°C, and the shelf life should not exceed 3 weeks; at -20°C, they can be stored for half a year and can be stored at -80°C for a long time, but repeated freezing and thawing is strictly prohibited.

[0075] (3) Transportation: Use a curling pot with ice or a foam box with dry ice for sealed transpor...

Embodiment 2

[0163] Refer to Example 1 for the specific detection method of this example, the difference lies in the composition of the kit of this example as shown in the following table:

[0164] Name

[0165] The specifications shown in the table: Each chip can detect 12 sheep at the same time. 144 sheep / box. Among them, extract 1 and extract 2 are contained in the "blood cell genomic DNA extraction kit" produced by Shanghai Bai Ao Technology Co., Ltd., the product number: BST01013, you can extract sheep genomic DNA according to the instructions; pre-hybridization solution and wash Solution 1 uses the chip hybridization kit produced by Shanghai Baiao Technology Co., Ltd. The product number of the chip hybridization kit: BST05010, produced by Shanghai Baiao Technology Co., Ltd.; Among them, washing solution 2, washing solution 3, antibody solution, and color developing solution are used The chip color reagent kit of Shanghai Baiao Technology Co., Ltd. was used for color development test...

Embodiment 3

[0167] Refer to Example 1 for the specific detection method of this example, the difference lies in the composition of the kit of this example as shown in the following table:

[0168] Name

[0169] The specifications shown in the table: Use one chip to detect 6 sheep. Among them, extract 1 and extract 2 are contained in the "blood cell genomic DNA extraction kit" produced by Shanghai Bai Ao Technology Co., Ltd., the product number: BST01013, you can extract sheep genomic DNA according to the instructions; pre-hybridization solution and wash Solution 1 uses the chip hybridization kit produced by Shanghai Baiao Technology Co., Ltd. The product number of the chip hybridization kit: BST05010, produced by Shanghai Baiao Technology Co., Ltd.; Among them, washing solution 2, washing solution 3, antibody solution, and color developing solution are used The chip color reagent kit of Shanghai Baiao Technology Co., Ltd. was used for color development test. The product code of the chip ...

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Abstract

The invention relates to a gene analysis and detection product, in particular to a detection kit and a detection method which are applicable to the polymorphism of a sheep BMPR-1B gene, namely A746G. The detection kit for the polymorphism of the sheep BMPR-1B gene, namely the A746 mainly comprises amplification solution, at least one gene chip, hybridization buffer solutions I and II, prehybridization solution, Taq enzyme, and a hybridization chromogenic reagent. In addition, the method for detecting the polymorphism of sheep BMPR-1B gene A746G by utilizing the kit comprises the following steps of: preparing a plurality of ovine chromosomal deoxyribonucleic acids (DNA) for later use; assembling the kit; amplifying by a polymerase chain reaction (PCR) method; performing metamorphosis treatment; prehybridizing and hybridizing; and detecting a hybridization signal. The kit has high sensitivity and accuracy rate, is easy to operate, can effectively improve work efficiency, is economic and practical, and is applicable to the detection of the polymorphism of the sheep BMPR-1B gene, namely the A746G; and by the detection method, large-scale detection is realized, and the method is simple, convenient and practical, and greatly improves work efficiency.

Description

Technical field [0001] The invention relates to a gene analysis and detection product, and more specifically, it is suitable for a detection kit and a detection method for the A746G polymorphism of the BMPR-1B gene of sheep. Background technique [0002] Gene chip is one of the most important technological developments with the most contemporary characteristics in the high-tech field in recent years. It is a gene probe (oligonucleotide probe, cDNA clone, PCR product, etc.) that can reflect a large amount of gene information in the sample, and orderly fixed on a solid support (such as aldehyde group, amino group, sulfhydryl group, carboxyl group, etc.) An array is formed on the group-modified glass slide or silicon wafer, nylon membrane, nitrocellulose membrane, and the actual sample (or amplified product) is hybridized. Only one experiment is needed to obtain all the waiting samples in high throughput. Check gene information. The characteristics of parallel detection and high i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 杨华钟发刚朱滨王新华刘守仁杨永林沈敏
Owner XINJIANG ACADEMY OF AGRI & RECLAMATION SCI
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