Preparation method of probiotics agent capable of lowering blood fat

A probiotic and blood lipid-lowering technology, applied in the field of blood-lipid-lowering functional food or medicine, to achieve gastrointestinal function, simple preparation method, and blood-lipid-lowering effect

Active Publication Date: 2011-08-31
隗雨竹
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the announcement number is CN101919875 invention patent application, which discloses that the polysaccharides of Enteromorpha and Lentinan can improve the body’s immunity and anti-tumor effects, but there is no report about Enteromorpha’s blood lipid-lowering effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A preparation method of a blood fat-lowering probiotic agent. First, the Bacillus licheniformis BLc strain (preservation number is CGMCC No: 4324) is expanded and cultivated. The seed medium is composed of 600 grams of sucrose, 300 grams of casein, 50 grams of NaCl, and 2 grams of MnSO 4 After mixing, add water to a total weight of 10,000 grams, stir evenly to make a seed medium, insert Bacillus licheniformis BLc strain, and cultivate at 37°C for 8-10 hours, and check the number of viable bacteria under the microscope. If there are too many, it needs to be diluted to 1 ml The number of live bacteria in the bacterial solution is 5×10 5 ~5×10 7 1, to obtain the Bacillus licheniformis BLc strain bacterium liquid and save it for later use; then prepare the fermentation medium: 8 kilograms of Enteromorpha dry powder, 3 kilograms of yeast extract, 0.3 kilogram of NaCl, 0.4 kilogram of K 2 HPO 4 ·3H 2 O, 0.02 kg NaH 2 PO 4 After mixing, add water to a total weight of 100...

Embodiment 2

[0021] It is basically the same as Example 1, except that the seed culture medium is made of 500 grams of maltose, 200 grams of yeast extract, 30 grams of NaCl, 1 gram of MnSO 4 After mixing, add water to a total weight of 10,000 grams; the fermentation medium consists of 4 kg of Enteromorpha dry powder, 1 kg of yeast extract, 0.1 kg of NaCl, 0.2 kg of K 2 HPO 4 ·3H 2 O, 0.01 kg NaH 2 PO 4 After mixing, add water to a total weight of 100 kg to prepare; add 8 kg of Bacillus licheniformis BLc strain bacterial liquid to the fermentation medium, the amount of skimmed milk powder added is 1% of the sediment weight, and the solid content is 10-15%. The amount of β-cyclodextrin added was 5% of the weight of the precipitate.

Embodiment 3

[0023] Substantially the same as Example 1, the only difference is that the seed medium is made of 800 gram soluble starch, 400 gram gelatin, 60 gram NaCl, 3 gram MnSO4 After mixing, add water to a total weight of 10,000 grams; the fermentation medium consists of 10 kg of Enteromorpha dry powder, 4 kg of yeast extract, 0.5 kg of NaCl, 0.6 kg of K 2 HPO 4 ·3H 2 O, 0.03 kg NaH 2 PO 4 After mixing, add water to a total weight of 100 kg to prepare; add 12 kg of Bacillus licheniformis BLc strain bacterial liquid to the fermentation medium, the amount of skimmed milk powder added is 5% of the weight of the sediment, and the solid content is 20-25%. The amount of β-cyclodextrin added was 8% by weight of the precipitate.

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PUM

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Abstract

The invention discloses a preparation method of a probiotics agent capable of lowering blood fat. Bacillus licheniformis BLc strains are activated and are subjected to scale-up culture by seed culture; the Bacillus licheniformis BLc strains subjected to scale-up culture are inoculated into a fermentation medium; NaCl, K2HPO4.3H2O and NaH2PO4 in the fermentation medium have actions on accelerating the secretion of various enzymes of the protease, the lipase, the amylase and the like of the BLc strains and on improving activity; the activity of the enzyme can be improved if the pH of the fermentation medium is controlled to 6-7, Enteromorpha is degraded, and a large quantity of Bacillus licheniformis BLc strain sporophyte is contained; after spray drying, the sporophyte temporarily looses activity; after being eaten, the sporophyte revives when entering the stomach and intestine and secretes various enzymes so as to accelerate gastrointestinal foods to decompose and decompose cholesterol, fat and the like; meanwhile, antagonism can be carried out on enteropathogenic bacteria; and the Enteromorpha degradation product has the action on lowering blood fat, resisting lipid peroxidation and preventing fatty liver. The invention provides the preparation method of probiotics agent capable of lowering blood fat, which has the advantages of low raw material cost and simple preparation method and has an action on accelerating the gastrointestinal function.

Description

technical field [0001] The invention relates to a blood fat-lowering functional food or medicine, in particular to a preparation method of a blood fat-lowering probiotic agent. Background technique [0002] With the continuous improvement of people's living standards, there are more and more sub-healthy people, especially the number of blood three highs is increasing. Blood lipid is an important indicator of cardiovascular disease. Elevated blood lipids will increase the possibility of atherosclerosis and cardiovascular disease outbreaks, and can cause various complications in cardiovascular and other organs, such as concurrent arteriosclerosis and heart disease. , Insufficient blood supply to the brain and other problems, abnormal liver function, uremia, hyperlipidemia, pancreatitis, etc. can also be complicated by symptoms of hyperlipidemia. Cholesterol and triglycerides have two parts, one part is eaten outside, this part is not the main part of the increase in human cho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/29A61K35/74A61P3/06C12N1/20C12R1/10A23L33/00A61K35/742
Inventor 苏秀榕高翔徐嘉杰
Owner 隗雨竹
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