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Method for preparing diaporthein B and application of diaporthein B to preparation of antitumor medicaments

A technology of anti-tumor drugs and seeds, applied in the field of medicine and biology, can solve the problems such as the anti-tumor activity of the unrelated compound diaportheinB, achieve the effect of convenient drug source, solve the problem of drug source, and shorten the fermentation time

Inactive Publication Date: 2011-08-31
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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Problems solved by technology

[0008] But so far, in the literature reports at home and abroad, there is no report that the compound diaporthein B has anti-tumor activity

Method used

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  • Method for preparing diaporthein B and application of diaporthein B to preparation of antitumor medicaments
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  • Method for preparing diaporthein B and application of diaporthein B to preparation of antitumor medicaments

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Embodiment 1

[0024] 1. Isolation, purification and identification of marine fungus Eutypella scoparia FS26.

[0025] The marine fungus Eutypella scoparia (Eutypella scoparia) FS26 of the present invention is isolated and purified from sediments collected from the South China Sea (18°42.878′N, 111°2.797′E) at a depth of 139 meters, and obtained by ITS sequence Analysis and identification, the base sequence of the fragment in its ITS region is as shown in SEQ ID NO.1, and the GenBank gene accession number is: HM989831, through blast comparison, homologous analysis, the bacterial strain of the present invention belongs to the Curvularia spp. Eutypella scoparia) species, named Eutypella scoparia FS26. The bacterium was preserved in China Center for Type Culture Collection (CCTCC) on September 29, 2010, address: China. Wuhan. Wuhan University, and its preservation number is CCTCC NO: M 2010257.

[0026] 2. Liquid fermentation of Eutypella scoparia FS26.

[0027] The seed medium is semi-seawat...

Embodiment 2

[0040] Antitumor activity of compound diaporthein B was tested by MTT method [Liu Jianwen. Pharmacological Experimental Methodology, Beijing: Chemical Industry Press, 2007, 29-32]

[0041] 1. Test reagents:

[0042] The compound diaporthein B prepared by the present invention was dissolved in dimethyl sulfoxide (DMSO) to obtain a mother solution with a concentration of 50 mg / mL, and then diluted to the required concentration with RPMI-1640 medium.

[0043] The positive control was cisplatin aqueous solution.

[0044] The tumor cell lines used in this experiment were glioma cell SF-268, breast cancer cell MCF-7 and large cell lung cancer cell NCI-H460.

[0045] 2. Experimental method:

[0046] NCI-H460, SF-268, and MCF-7 cells in the logarithmic growth phase were taken respectively, digested with trypsin, stained with trypan blue and counted. After the trypan blue exclusion test detected that the cell viability was greater than 95%, they were cultured with fresh RPMI-1640 Ba...

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Abstract

The invention discloses a method for preparing diaporthein B and application of the diaporthein B to the preparation of antitumor medicaments. In the method, the diaporthein B with antitumor activity is separated from a liquid fermentation culture of a marine fungus, namely Eutypella scoparia FS26. The method has the advantages that culture conditions are mild, a culture medium is easy to prepare, fermentation time is short, separation and purification steps are simple, the mass production can be realized under manual control conditions and the like, and the problem of medicament sources can be solved more conveniently. Through the experiment, IC50 values of the diaporthein B for a glioma cell SF-268, a breast cancer cell MCF-7 and a large-cell lung cancer cell NCI-H460 are 92mu M, 44mu M and 9.9mu M respectively, the diaporthein B has remarkable antitumor activity, and IC50 values of a positive control, namely cis-platinum for the three tumor cell strains are 4.6mu M, 4.1mu M and 3.0mu M respectively. Therefore, a candidate compound is provided for researching and developing novel antitumor medicaments, and a scientific evidence is provided for developing and utilizing natural active substances which are derived from marine microorganisms.

Description

Technical field: [0001] The invention belongs to the field of medical biotechnology, and specifically relates to a method for preparing diaporthein B by fermenting marine fungus Eutypella scoparia FS26, and the application of compound diaporthein B in the preparation of antitumor drugs and as active ingredients of antitumor drugs . Background technique: [0002] Malignant tumors, often called cancer, are a group of common and frequently-occurring diseases that seriously threaten human health. WHO estimates that without intervention, 84 million people will die from cancer between 2005 and 2015. Moreover, bad habits such as population aging, increased smoking, reduced physical activity, and lack of vegetables and fruits in the diet may further lead to a sharp increase in the number of cancers worldwide. According to the research report "World Cancer Report" of the International Center for Cancer Research, according to the current trend of cancer incidence, the incidence of c...

Claims

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Application Information

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IPC IPC(8): C12P15/00A61K31/343A61P35/02
Inventor 李冬利孙利陈玉婵章卫民张庆波王磊
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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