Bacillus licheniformis CH15 for degrading straws and bacterial agent thereof

A technology of Bacillus licheniformis and CH15, which is applied to the preparation of bacteria and organic fertilizers, and methods based on microorganisms, can solve undiscovered problems, improve the degradation effect, improve the level of soil organic matter and humus, and improve soil organic matter and humus horizontal effect

Inactive Publication Date: 2011-09-07
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bacillus licheniformis CH15 is a newly isolated strain that degrades cellulosic lignin, and no reports have been found about Bacillus licheniformis as a microbial agent for efficient straw degradation

Method used

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  • Bacillus licheniformis CH15 for degrading straws and bacterial agent thereof
  • Bacillus licheniformis CH15 for degrading straws and bacterial agent thereof
  • Bacillus licheniformis CH15 for degrading straws and bacterial agent thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Preparation of straw-degrading microbial bacterial agent containing Bacillus licheniformis CH15

[0036] (1) Fermentation tank culture solution formula

[0037] Soluble starch 1%, soybean meal 1%, corn flour 0.5%, glucose 0.1%, sucrose 1%, yeast powder 0.5%, (NH 4 ) 2 SO 4 0.3%, K 2 HPO 4 0.02%, NaCl 2.5%, MgSO 4 ·7H 2 O 0.01%, CaCO 3 0.05%, FeSO 4 0.0001%, pH 7.0, stirred.

[0038] (2) Training process

[0039] Bacillus licheniformis CH15 was inoculated on beef juice peptone medium, cultured at 28-30°C for 48h, then inserted into a 500mL Erlenmeyer flask liquid medium, and cultured at 30°C and 180r / min for 36h. Then insert in the 15L seed tank by 1% inoculum amount, at 220r / min, pH7.0, under aeration 0.6vvm, after cultivating 24h, then pack in the fermenter of 100L by 5% inoculum amount, in 220r / min, pH 7.0, ventilation 0.7vvm, cultivated for 3 days. After the fermentation was completed, the Bacillus licheniformis CH15 fermented liquid was add...

experiment example 1

[0040] Experimental example 1 Effect of the straw-degrading microbial agent of the present invention on the degradation effects of hemicellulose, cellulose and lignin in wheat straw covered by the ground

[0041] Pot and pot experiments were conducted to study the effect of using degrading bacteria agent on the ground covering straw. Test strains: ① Bacillus Weidenstätten AS2; ② Acinetobacter calcoacetate G22; ③ Bacillus licheniformis CH15 of the present invention, wheat straw was used in the experiment for a period of 6 weeks.

[0042] The preparation method of the bacterial agent containing the bacterial strain AS2 and the bacterial agent containing the bacterial strain G22 is the same as that of the bacterial agent containing the bacterial strain CH15, specifically refer to Example 1.

[0043] When wheat straw was treated with bacterial agents containing strains AS2, G22 and CH15 for 2 weeks, the lignin content of straw decreased by 66.6%, 67.9% and 21.9% respectively compa...

experiment example 2

[0052] Experimental example 2 Effect of straw degrading microbial agent on the degradation effect of hemicellulose, cellulose and lignin in corn straw covered by ground

[0053] Pot and pot experiments were conducted to study the effect of using degrading bacteria agent on the ground covering straw. The tested strains: ① Bacillus Weidenstätten AS2; ② Acinetobacter calcoacetate G22; ③ Bacillus licheniformis CH15 of the present invention, corn stalks were used in the experiment for a period of 6 weeks.

[0054]The preparation method of the bacterial agent containing the bacterial strain AS2 and the bacterial agent containing the bacterial strain G22 is the same as that of the bacterial agent containing the bacterial strain CH15, specifically refer to Example 1.

[0055] When corn stalks were treated with AS2, G22 and CH15 bacteria agents for 2 weeks, the degradation effects of the three straw-degrading bacteria agents on cellulose were different, and the degradation effect of CH...

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Abstract

The invention provides bacillus licheniformis CH15 for efficiently degrading crop straws and a bacterial agent thereof, relating to the bacillus licheniformis CH15. The bacillus licheniformis CH15 can be used for efficiently degrading cellulose, lignin and hemicellulose of straws, has the capability of degrading various crop straws, is suitable for quick degradation of southern paddy straws and northern wheat straws and corn straws, has the effects of promoting field in-situ degradation and returning of the crop straws and increasing the functions of soil organic matters and humus, and provides a high-efficiency strain for production and application of the straw degradation bacterial agent.

Description

technical field [0001] The invention belongs to the field of microbial inoculations, in particular to a microbial inoculation containing Bacillus licheniformis CH15 for efficiently degrading straw. Background technique [0002] my country is rich in crop straw resources, with a huge quantity and various types. The annual output of crop straw is 700-800 million tons. Because the straw is not easy to decompose in a short period of time, and the utilization efficiency is low, most of the straw is discarded or burned. In areas where planting is the mainstay, crop straw has become an obstacle in production. Therefore, straw burning spreads all over the vast countryside, which causes environmental pollution and serious waste of organic resources. According to statistics, the crop straw used for returning to fields and organic fertilizers in the country only accounts for about 20% of the total, incineration accounts for 17%, and 50% remains unused. [0003] The summer drought and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/02C05F17/00C12R1/10
CPCY02W30/40
Inventor 范丙全任萍
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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