Streptomyces antibioticu and biological preparation prepared from active product thereof and application
An anti-Streptomyces and biological preparation technology, applied in the directions of biocides, chemicals for biological control, applications, etc., can solve problems such as failure and reduction of chemical pesticide control effects, and achieve easy operation, emulsification and stability. Good, cost-effective results
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Embodiment 1
[0043] Example 1 Screening of antagonistic actinomycetes
[0044] More than 2,000 actinomycetes were isolated from sea mud off the coast of Guangdong Province, and the strains with good inhibitory activity against Phytophthora capsici and Capsicum anthracnose were screened.
[0045] The screening method adopts confrontation culture method.
[0046] ① Phytophthora capsici (preliminary study on the identification method of capsicum anti-epidemic disease, Tianjin Agricultural Sciences, 2010, 16 (5): 100-103) was inoculated with 6mm bacterial block into V8 solid medium, and capsicum anthracnose 3 pathogenic ribosomal gene Sequence determination and analysis of the ITS region, Southwest Agricultural Journal, 2007, 20(6): 1234-1236) inoculated 6mm bacterial blocks onto a PDA solid medium plate and cultured at 26-28°C. V8 solid medium components are imported from the United States V 8 Vegetable juice 100mL, CaCO 3 0.2g, agar 18g, water to 1000mL; PDA solid medium is potato 200g, ...
Embodiment 2
[0050] Example 2 Classification and Identification of Actinomycetes H74-18
[0051] (1) Morphological observation
[0052] The strain was cultured as inserts, and the hyphae in the substrate, the aerial hyphae, and whether sporocysts were generated; the shape of the sporocydus; whether there were spores formed; whether the substrate mycelium was broken, etc. Samples were taken on 7d, 14d, 21d and 28d, respectively, and observed with an optical microscope and a scanning electron microscope (such as figure 1 and figure 2 shown).
[0053] The results of insert culture are: on Gao’s No. 1 medium, the basal hyphae and aerial hyphae are abundant, the basal filaments are not broken, the spore filaments are attached to the aerial hyphae, the spore filaments are flexible, and the spores are smooth and cylindrical The number of mature spore chain spores is about 8, and no structures such as cysts and spore nuclei have been observed.
[0054] (2) Cultivation characteristics
[0055...
Embodiment 3
[0091] Pot control effect test of metabolite preparations of Streptomyces subsp.
[0092] (1) Preparation of biological agents: active product antimycin A 18 (1) 0.2g of powder, 0.5g of emulsifier (Tuwen-80), 4.3g of methanol; mix according to the above ratio and stir evenly.
[0093] (2) The pot experiment was carried out in a greenhouse. Each plastic pot was filled with 0.5 kg of natural air-dried vegetable garden soil, and each pot was transplanted with 2 strong pepper seedlings at the 4-leaf stage or 2 healthy cucumber seedlings at the 4-leaf stage, and the growth was normal after 5 days of transplanting for testing. Phytophthora capsici and Phytophthora cucumber FR-58 (Effect of the new fungicide flumorph on the synthesis and distribution of main components of the cell wall of Phytophthora cucumber. Chemical Journal of Chinese Universities, 2007, 28(4): 658-662) In the V8 culture medium (imported from the United States, V 8 Vegetable juice 150mL, CaCO 3 0.2g, water 85...
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