Streptomyces antibioticu and biological preparation prepared from active product thereof and application

An anti-Streptomyces and biological preparation technology, applied in the directions of biocides, chemicals for biological control, applications, etc., can solve problems such as failure and reduction of chemical pesticide control effects, and achieve easy operation, emulsification and stability. Good, cost-effective results

Inactive Publication Date: 2011-09-07
PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Because chemical pesticides can quickly and effectively inhibit or kill plant pathogenic bacteria, people have long relied on chemical pesticides to prevent and cont

Method used

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  • Streptomyces antibioticu and biological preparation prepared from active product thereof and application
  • Streptomyces antibioticu and biological preparation prepared from active product thereof and application
  • Streptomyces antibioticu and biological preparation prepared from active product thereof and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Screening of antagonistic actinomycetes

[0044] More than 2,000 actinomycetes were isolated from sea mud off the coast of Guangdong Province, and the strains with good inhibitory activity against Phytophthora capsici and Capsicum anthracnose were screened.

[0045] The screening method adopts confrontation culture method.

[0046] ① Phytophthora capsici (preliminary study on the identification method of capsicum anti-epidemic disease, Tianjin Agricultural Sciences, 2010, 16 (5): 100-103) was inoculated with 6mm bacterial block into V8 solid medium, and capsicum anthracnose 3 pathogenic ribosomal gene Sequence determination and analysis of the ITS region, Southwest Agricultural Journal, 2007, 20(6): 1234-1236) inoculated 6mm bacterial blocks onto a PDA solid medium plate and cultured at 26-28°C. V8 solid medium components are imported from the United States V 8 Vegetable juice 100mL, CaCO 3 0.2g, agar 18g, water to 1000mL; PDA solid medium is potato 200g, ...

Embodiment 2

[0050] Example 2 Classification and Identification of Actinomycetes H74-18

[0051] (1) Morphological observation

[0052] The strain was cultured as inserts, and the hyphae in the substrate, the aerial hyphae, and whether sporocysts were generated; the shape of the sporocydus; whether there were spores formed; whether the substrate mycelium was broken, etc. Samples were taken on 7d, 14d, 21d and 28d, respectively, and observed with an optical microscope and a scanning electron microscope (such as figure 1 and figure 2 shown).

[0053] The results of insert culture are: on Gao’s No. 1 medium, the basal hyphae and aerial hyphae are abundant, the basal filaments are not broken, the spore filaments are attached to the aerial hyphae, the spore filaments are flexible, and the spores are smooth and cylindrical The number of mature spore chain spores is about 8, and no structures such as cysts and spore nuclei have been observed.

[0054] (2) Cultivation characteristics

[0055...

Embodiment 3

[0091] Pot control effect test of metabolite preparations of Streptomyces subsp.

[0092] (1) Preparation of biological agents: active product antimycin A 18 (1) 0.2g of powder, 0.5g of emulsifier (Tuwen-80), 4.3g of methanol; mix according to the above ratio and stir evenly.

[0093] (2) The pot experiment was carried out in a greenhouse. Each plastic pot was filled with 0.5 kg of natural air-dried vegetable garden soil, and each pot was transplanted with 2 strong pepper seedlings at the 4-leaf stage or 2 healthy cucumber seedlings at the 4-leaf stage, and the growth was normal after 5 days of transplanting for testing. Phytophthora capsici and Phytophthora cucumber FR-58 (Effect of the new fungicide flumorph on the synthesis and distribution of main components of the cell wall of Phytophthora cucumber. Chemical Journal of Chinese Universities, 2007, 28(4): 658-662) In the V8 culture medium (imported from the United States, V 8 Vegetable juice 150mL, CaCO 3 0.2g, water 85...

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Abstract

The invention discloses streptomyces antibioticu and biological preparation prepared from an active product thereof and application. The streptomyces antibioticu is a strain which is screened from more than 2,000 marine actinomycetes and has good inhibiting activity on phytophthora capsici, phytophthora cucumisativus, colletorichum cucumisativus and colletotrichum capsici; and the name of the strain is streptomyces antibioticu H74-18, the strain was collected in the China Center for Type Culture Collection on December 27th, 2010, and the collection number is CCTCC NO: M2010364. Antimycin A18(1) with bacteriostatic activity is separated from the strain, the molecular formula of the antimycin A18(1) is C28H41N2O9, and the molecular weight of the antimycin A18(1) is 549.3. The antimycin A18(1), an emulsifier and methanol are mixed to form the biological preparation with good stability. In 12 days after the biological preparation is sprayed, under the application times of 500x, the effect of preventing and controlling the phytophthora capsici is 90.00 percent, the effect of preventing and controlling the phytophthora cucumisativus is 86.67 percent, the effect of preventing and controlling the colletotrichum capsici is 86.67 percent and the effect of preventing and controlling the colletorichum cucumisativus is 90.00 percent.

Description

technical field [0001] The invention belongs to the field of biopesticide research, and in particular relates to a biological preparation made from anti-streptomyces and its active product and its application. Background technique [0002] Because chemical pesticides can quickly and effectively inhibit or kill plant pathogenic bacteria, people have long relied on chemical pesticides to prevent and control plant diseases, which has also significantly increased the resistance of pathogenic bacteria, resulting in reduced or even invalid control effects of chemical pesticides. In actual production, different means should be combined for comprehensive prevention and control to reduce the dependence on chemical pesticides and delay the emergence of chemical pesticide resistance. [0003] Agricultural antibiotics are a class of high-efficiency, broad-spectrum, and non-polluting pesticides. According to statistics, 67% of antibiotic products in the world come from microorganisms, a...

Claims

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Application Information

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IPC IPC(8): C12N1/20C07D321/00C12P17/08A01N43/24A01P3/00C12R1/48
Inventor 蒲小明林壁润沈会芳李培谦潘群英
Owner PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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