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Method for half quantitatively and fast detecting contents of delta 9-THC (tetrahydrocannabinol) in cannabis plants

A 9-THC, semi-quantitative technology, applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of difficult basic unit promotion, slow inspection speed, high inspection cost, easy to master and configure, improve accuracy, detect little interference effect

Inactive Publication Date: 2011-09-07
云南省农业科学院经济作物研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the similar chemical properties among nearly a hundred cannabinol derivatives in cannabis, giving △ 9 -The isolation and further quantitative analysis of THC caused difficulties
[0004] In the existing domestic and foreign literature, there is a qualitative analysis method for cannabis based on the principle of chemical color development, but only for color development of cannabinoids, so as to qualitatively determine whether it is cannabis or cannabis drugs; there are methods based on gas chromatography, liquid chromatography, etc. The instrument method, can carry out △ 9 - Accurate quantitative analysis of THC, but the disadvantages are that the inspection speed is slow, the analysis operation is cumbersome, the purchase of instruments is expensive, special training is required, and the inspection cost is high (a sample needs more than 400 yuan), which is difficult to promote in grassroots units; Cannabis qualitative method of immunoassay technology, which is mainly used in fluid biological samples such as saliva, blood, urine, etc.△ 9 -Qualitative detection of THC (CN200910004736.5)
None of these methods were able to control the △ in the cannabis plant 9 - Whether the THC content exceeds or falls below 0.3% for rapid and accurate determination

Method used

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  • Method for half quantitatively and fast detecting contents of delta 9-THC (tetrahydrocannabinol) in cannabis plants
  • Method for half quantitatively and fast detecting contents of delta 9-THC (tetrahydrocannabinol) in cannabis plants
  • Method for half quantitatively and fast detecting contents of delta 9-THC (tetrahydrocannabinol) in cannabis plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Take 1g of newly collected fresh cannabis leaves and bake them at 140°C for 5 minutes to quickly decarboxylate THCA in cannabis into △ 9 -THC, grind the sample until it passes through a 0.425mm sieve, weigh 0.050g of fine powder, and use 1ml of n-hexane to ultrasonically extract it for 10min as the test solution; take another 150μg / ml (now prepared) Δ 9 - THC standard solution (equivalent to THC content = 0.3%), as the reference solution; in an environment of 10~30 ° C, take 1 μl of the test solution and the reference solution respectively and spot on the same piece of polyamide thin-layer chromatography material The sample was developed with petroleum ether: ethanol = 12: 1, and the height of development was 6cm, and then 1.5mg / ml fast blue BB salt dichloromethane solution (prepared) was used as the color developer for color development; the test solution With reference substance solution Δ 9 The corresponding chromatographic positions of -THC all show rose red spots,...

Embodiment 2

[0057] Take 1g of newly collected fresh cannabis leaves, bake them at 140°C for 5min, grind them until they pass through a 0.425mm sieve, weigh 0.050g of fine powder, and use 1ml of petroleum ether for ultrasonic extraction for 8min as the test solution; The preparation is the same as in Example 1; take 1 μl each of the test solution and the reference solution to spot on the same silica gel thin-layer chromatography material, develop with n-hexane:ether=4:1, and the height of development is 7cm, and then use 1.25mg / ml fast blue B salt chloroform solution (prepared now) is used as the chromogenic agent for color development; the test solution and the reference solution Δ 9 -The corresponding chromatographic positions of THC all show rose red spots, and the color of the test product is obviously lighter than that of the reference product. The judgment result: the test product Δ 9 -THC content is less than 0.3%, and the detection time is 27 minutes.

Embodiment 3

[0059] Take 1g of freshly collected cannabis leaves and bake them at 90°C for 10 minutes to rapidly decarboxylate THCA in cannabis into △ 9 -THC, the sample is ground until it passes through a 0.425mm sieve, and 0.050g fine powder is weighed, and ultrasonically extracted with 1ml of n-hexane for 10min, as the test solution; the preparation of the reference solution is the same as in Example 1; Sample operation is the same as embodiment 2, develops with sherwood oil: ethyl acetate=7:1, and development height is 6cm, and color developing operation is the same as embodiment 2; Need testing solution and reference substance solution Δ 9 -The corresponding chromatographic positions of THC all show rose red spots, and the color of the test product is obviously lighter than that of the reference product. The judgment result: the test product Δ 9 -THC content is less than 0.3%, and the detection time is 29 minutes.

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Abstract

The invention discloses a method for half quantitatively and fast detecting contents of delta 9-THC (tetrahydrocannabinol) in cannabis plants. The method comprises the steps of: baking and smashing cannabis plants to be fine powder; ultrasonically extracting the powder by an organic solvent to obtain a test article solution; taking a standard solution containing 0.3% of delta 9-THC as a referencesolution; and after pointing samples, expanding the solutions and developing colours, judging the content of the delta 9-THC in the test article via observing the colour differences of the test article and the reference article. Water in the cannabis plants is removed fast by heating at a high temperature; delta 9-THC is fast transformed and formed from carboxylic acid precursors via decaboxylization; and the problem that the delta 9-THC in newly collected cannabis plant sample is difficult to be detected is solved. The method is fast, simple, easily operated and has low detection cost, and can fast and half quantitatively detect cannabis plants; the method can be used for fast screening in laboratory; and the basic unit is mastered and configured easily; and a good application perspective is provided.

Description

technical field [0001] The invention belongs to the technical field of plant component detection, in particular to a semi-quantitative rapid detection of △ in cannabis plants 9 - Method of THC content. Background technique [0002] marijuana( Cannabis sativa L.) contains a hallucinogenic substance tetrahydrocannabinol (THC, tetrahydrocannabinol), which is easily used by criminals to refine drugs and cause social harm. Due to the spread of illegal drugs, many countries have taken measures to restrict the cultivation of cannabis. According to the provisions of the international drug control convention, the THC content in the dry matter of cannabis > 0.3% is used as the basis for judging the drug cannabis. The fibers, seeds, stems and even whole plants of industrial hemp with a THC content of less than 0.3% have high economic development and utilization value. Its utilization can involve papermaking, textiles, building materials, industrial oil, functional health food, c...

Claims

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Application Information

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IPC IPC(8): G01N30/90
Inventor 郭孟璧郭鸿彦杨明许艳萍张庆滢陈璇
Owner 云南省农业科学院经济作物研究所
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