Screening method of selfing infertile paspalum vaginatum by inducing somatic mutants with colchicine

Abstract

The invention relates to a screening method of selfing infertile paspalum vaginatum by inducing somatic mutants with colchicine, belonging to the field of agricultural biotechnologies. The screening method comprises the steps as follows: conducting enrichment culture on paspalum vaginatum granular callus for 20d-25d on a callus secondary culture medium, transferring the paspalum vaginatum granular callus to a callus secondary culture medium added with 500-1000g / L of colchicine and conducting mutagenesis culture for 24h, transferring the paspalum vaginatum granular callus to a differentiation culture medium and conducting culture for 35d-40d, transferring green seedlings and green shoots onto the differentiation culture medium to continue growing, transplanting regenerated plantlets with three complete leaves into a large field or basins, and checking the fertility during the mature period in the second year after transplanting, thus obtaining a self-fertility somatic mutant plantlet, wherein the harvested seeds can sprout 100% and grow into plantlets with complete roots, stems and leaves and the plantlets can tiller and form lawns normally. The screening method is applicable to the breeding of new variety of the paspalum vaginatum and has the advantages of being simple and convenient to operate, short in period and high in selection efficiency.

Description

1. Technical field [0001] The invention relates to a screening method for self-fertilizing guava colchicine-induced somatic cell mutants, belongs to the field of agricultural biotechnology, and is specially used for guava colchicine mutation breeding. 2. Background technology [0002] puffin ( Paspalum Vaginatum O. Swartz) is a Poaceae ( Graminea ) millet ( Panicea ) Paspalum ( Paspalum L.) perennial herbs, originating in Africa and America, which can be used as lawns, fodder and excellent grass species for improving saline-alkali land (Xie Xinming, Lu Xiaoliang. Excellent characteristics and utilization value of guava germplasm resources[J].South China Journal of Agricultural University, 2004, 25 (Supplement II): 64-67; Chen Xiaohong. Characteristics and maintenance and management techniques of seaside paspalum turf [J]. Shanghai Agricultural Science and Technology, 2007(4): 111). [0003] puffin ( Paspalum Vaginatum ) is a self-incompatible sexual diploid (Espinoz...

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Problems solved by technology

[0003] sea ​​paspalum ( Paspalum Vaginatum ) is a self-incompatible sexual diploid (Espinoza, F. and C.L. Quarin. Cytoembryology of Paspalum chaseanum and sexual diploid biotypes of two apomictic Paspalum species[J].Austral.J.Botany 1997, 45:871-877), because hybridization between different species within the same genus cannot produce viable seeds or the germination rate of seeds is only about 10% (Carpenter, J.A. Production and use of seed in seashore paspalum.J.Austral.Instit.Agri.Sci ., 1958, 24:252-256), conventional breeding work was slow

Using embryogenic callus as material, the selection of somatic mutants induced by colchicine is considered to be an effective new breeding method, but there is no official report on the screening method of somatic mutants induced by sea paspalum colchicine at home and abroad.

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