Formaldehyde biodegradation agent and preparation method thereof
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A technology of biodegradation agent and formaldehyde, applied in the direction of biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., can solve the problems of application limitations, consumption dependence on microorganisms, etc., achieve short action time, good stability, and increase production Effect
Active Publication Date: 2012-11-14
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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However, these microorganisms can only consume or degrade formaldehyde in the form of "in vivo", that is, the consumption of formaldehyde depends on the growth of microorganisms, and its application is greatly limited.
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[0039] 1) After the strain Pseudomonas IOFA1 was activated with LB medium, it was inoculated in liquid LB medium at a ratio of 1%, and cultured with shaking at 30°C until OD 600 = 1.0;
[0040] 2) Add formaldehyde solution to the LB medium to a final concentration of 100mg / mL; continue shaking culture at 30°C for 36h;
[0041] 3) centrifuging the Pseudomonas IOFA1 culture solution in step 2) at 4°C, removing the supernatant, and collecting the cells;
[0042] 4) Add 200 mL of sterile water pre-cooled at 4°C to Pseudomonas IOFA1, suspend the bacteria, and collect the suspension;
[0043] 5) Break up the bacteria in the suspension, centrifuge at 4°C to remove the precipitate, filter the supernatant with a 0.22 μm filter membrane, and freeze-dry, the obtained dry powder is the formaldehyde biodegradant. The crushing can be crushed by a homogenizer, the conditions are: the homogenization pressure is ...
Embodiment 2
[0095] Similar to Example 1, the difference is that in step 1), OD 600 The value is 1.3; in step 2), after adding formaldehyde, the final concentration of formaldehyde in the culture medium is 150 mg / mL, and the culture time is 48 hours.
Embodiment 3
[0097] Similar to Example 1, the difference is that in step 1), OD 600 The value is 1.5; in step 2), after adding formaldehyde, the final concentration of formaldehyde in the culture medium is 200mg / mL, the temperature for continuing cultivation is 30°C, and the cultivation time is 60h.
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Abstract
The invention discloses a formaldehyde biodegradation agent and a preparation method thereof, relates to the biodegradation agent and provides a pseudomonad, namely IOFA1, as well as the formaldehyde biodegradation agent and the preparation method thereof. The formaldehyde biodegradation agent is dry powder of pseudomonad IOFA1 thallus breaking solution. The formaldehyde biodegradation agent adopts formaldehyde dehydrogenase as a main component. The preparation method comprises the following steps: activating the pseudomonad IOFA1 by using a culture medium, inoculating the pseudomonad IOFA1 into the culture medium, and performing shaking culture; adding formaldehyde solution into the culture medium, and continuing performing the shaking culture; performing centrifugation on culture solution at the temperature of 4 DEG C, removing supernatant liquid and collecting thalli; adding 100ml of sterile water into the thalli to suspend the thalli, and collecting suspension; and performing the centrifugation after breaking the thalli in the suspension, removing precipitate, filtering the supernatant liquid for removing bacteria, performing freeze-drying to get the dry powder of the pseudomonad IOFA1 thallus breaking solution, and further performing purification to get the formaldehyde biodegradation agent.
Description
technical field [0001] The invention relates to a biodegradation agent, in particular to a formaldehyde biodegradation agent and a preparation method thereof. Background technique [0002] Formaldehyde is a widely used important chemical raw material, but it is easy to cause air pollution and pose a great threat to people's health. Indoor air formaldehyde pollution mainly comes from various man-made panels for furniture and decoration. It is prone to addition, oxidation, reduction, and polymerization reactions. It has high water solubility and high reactivity of biological macromolecules. Formaldehyde is absorbed or degraded at the contact parts. After entering the human body, it mainly reacts with the protein and nucleic acid of the human body, damages the DNA, causes the gene mutation of the cell nucleus, cross-links within the DNA single strand, cross-links between DNA and protein, and inhibits the repair of DNA damage; combines with amino groups to change the interior of...
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