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Liquid phase chip for detecting twelve pathogen antibodies in blood serum sample in high flux, and preparation method and using method thereof

One liquid phase chip and twelve kinds of technologies are applied in biological testing, material inspection products, and diseases transmitted by vectors, which can solve the problems of lack of models and evaluations, and achieve good detection results and flexible detection effects

Inactive Publication Date: 2014-04-16
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Claims
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Problems solved by technology

But whether the liquid-phase chip method can detect Eastern equine encephalitis virus antibody, West Nile virus antibody, yellow fever virus antibody, tularemia antibody, dengue virus antibody, avian influenza virus antibody, Platts virus antibody in human serum with high throughput. Rickettsia pathogen antibody, tick-borne encephalitis virus antibody, Q fever Rickettsia pathogen antibody, Venezuelan equine encephalitis pathogen antibody, equine encephalitis virus antibody, Western equine encephalitis virus antibody, a total of twelve antibodies, their quantitative detection What is the ability, there is still a lack of models and evaluation

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  • Liquid phase chip for detecting twelve pathogen antibodies in blood serum sample in high flux, and preparation method and using method thereof
  • Liquid phase chip for detecting twelve pathogen antibodies in blood serum sample in high flux, and preparation method and using method thereof
  • Liquid phase chip for detecting twelve pathogen antibodies in blood serum sample in high flux, and preparation method and using method thereof

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Embodiment Construction

[0063] Such as Figure 1 to Figure 5 As shown, the present invention relates to a high-throughput detection of Eastern Equine Encephalitis Virus Antibody, West Nile Virus Antibody, Yellow Fever Virus Antibody, Tularemia Antibody, Dengue Virus Antibody, Avian Influenza Antibody, Platts Rickettsia Antibody A liquid-phase chip preparation method for a total of twelve kinds of pathogenic antibodies including antibody to pathogen, tick-borne encephalitis virus, Q fever Rickettsia, Venezuelan equine encephalitis, equine equine encephalitis, equine equine encephalitis and Western equine encephalitis , detection and quantification methods are further described through the following specific embodiments, but the present invention is not limited by this embodiment in any way.

[0064] 1. Materials

[0065] 1. Relevant buffers for liquid phase chips:

[0066] (1) PBS buffer (pH7.4): NaCl 137mmol / L; KCl 2.7mmol / L; NaCl 2 HPO 4 10mmol / L; KH 2 PO 4 2mmol / L. Dissolve 8gNaCl, 0.2gKCl...

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Abstract

The invention discloses a liquid phase chip for detecting twelve pathogen antibodies in a blood serum sample in high flux, and a preparation method and a using method thereof. The method is high in detection capability, sensitivity and specificity and wide in dynamic range; during sample detection at one time, the twelve pathogen antibodies can be detected at the same time; detection items can be organized flexibly, so only the requested pathogen antibodies are detected; moreover, an open detection modeling platform which is represented by a Tula antibody and a Dengue antibody and used for detecting the liquid phase chip for quantitatively detecting microorganism antibodies is built.

Description

technical field [0001] The invention relates to a high-throughput detection of Eastern equine encephalitis virus antibody, West Nile virus antibody, yellow fever virus antibody, tularemia antibody, dengue virus antibody, avian influenza virus antibody, and Platts rickettsiae in serum samples Antibodies to pathogens, tick-borne encephalitis virus, Q fever Rickettsia, Venezuelan equine encephalitis, equine equine encephalitis, equine equine encephalitis, and Western equine encephalitis. Methods of preparation and methods of use. Background technique [0002] Eastern equine encephalitis virus is highly contagious and harmful to the population. Its clinical manifestations are very similar to Japanese encephalitis, and its case fatality rate is about 35%. The virus is currently listed as one of the main types of anti-biological terrorism by the international community, and it is a type of virus managed in China, which has attracted much attention. [0003] West Nile fever is an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/96G01N21/64
CPCY02A50/30
Inventor 王静杨永莉杨宇
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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