Liquid phase chip for detecting twelve pathogen antibodies in blood serum sample in high flux, and preparation method and using method thereof
One liquid phase chip and twelve kinds of technologies are applied in biological testing, material inspection products, and diseases transmitted by vectors, which can solve the problems of lack of models and evaluations, and achieve good detection results and flexible detection effects
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[0063] Such as Figure 1 to Figure 5 As shown, the present invention relates to a high-throughput detection of Eastern Equine Encephalitis Virus Antibody, West Nile Virus Antibody, Yellow Fever Virus Antibody, Tularemia Antibody, Dengue Virus Antibody, Avian Influenza Antibody, Platts Rickettsia Antibody A liquid-phase chip preparation method for a total of twelve kinds of pathogenic antibodies including antibody to pathogen, tick-borne encephalitis virus, Q fever Rickettsia, Venezuelan equine encephalitis, equine equine encephalitis, equine equine encephalitis and Western equine encephalitis , detection and quantification methods are further described through the following specific embodiments, but the present invention is not limited by this embodiment in any way.
[0064] 1. Materials
[0065] 1. Relevant buffers for liquid phase chips:
[0066] (1) PBS buffer (pH7.4): NaCl 137mmol / L; KCl 2.7mmol / L; NaCl 2 HPO 4 10mmol / L; KH 2 PO 4 2mmol / L. Dissolve 8gNaCl, 0.2gKCl...
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