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High-density culture method for lactic acid bacteria

A technology for high-density culture and lactic acid bacteria, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of complex and cumbersome equipment and instruments

Active Publication Date: 2011-09-21
JUNLEBAO DAIRY GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods are usually mainly aimed at the production of lactic acid bacteria powder, and the equipment and instruments used are complex and cumbersome, and have certain limitations.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Pick Lactobacillus plantarum (CICC23506) and activate it in MRS medium for two generations, then streak it on the plate of MRSA medium (i.e. solid MRS medium), culture at 37°C for 24 h, and then culture in MRS medium at 37°C for 24 h. h, resulting in a concentration of 10 6 cfu / mL bacterial suspension. Use a pipette gun to draw 1 mL of the above bacterial suspension, that is, inoculate 100 mL of fermentation medium and 100 mL of MRS medium at an inoculum size of 1%, and incubate at 37 °C for 24 h.

[0027] The above two-generation activation process is as follows: pick Lactobacillus plantarum (JMCC0002) and culture it in the MRS medium at 37°C for 24 hours, then inoculate the bacterial suspension into the MRS medium with an inoculum of 1%, and culture it at 37°C. 24 h.

[0028] The formula of the above-mentioned fermentation medium is as follows: 0.5% soybean peptide, 4% compound fruit and vegetable juice, and the balance is reconstituted skim milk reduced by the rat...

Embodiment 2

[0032] After picking Lactobacillus plantarum (CICC22856) and activating it in MRS medium for two generations, pipette 1 mL of the bacterial suspension, that is, inoculate 100 mL of fermentation medium and 100 mL of basal medium at an inoculation volume of 1%. That is, cultured in MRS medium for 24 h at 35 °C.

[0033] The formula of the above-mentioned fermentation medium is as follows: soybean peptide 1%, compound fruit and vegetable juice 3%, the balance is raw milk, pH 6.8. Among them, the molecular weight of soybean peptide is 180 Da to 800 Da, and the number of amino acids contained is 2 to 6. The composition of compound fruit and vegetable juice is: longan juice 55%, mango juice 10%, apple juice 5%, pineapple juice 5%, banana juice 5%, burdock juice 10%, carrot juice 10%.

[0034] The base medium is the same as in Example 1.

[0035] The number of live bacteria in the fermented liquid obtained by cultivating method of the present invention and cultivating with basal me...

Embodiment 3

[0037] After picking Lactobacillus casei (CICC22852) and activating it in MRS medium for two generations, pipette 1 mL of the bacterial suspension, that is, inoculate 100 mL of fermentation medium and 100 mL of basal medium with a 1% inoculation amount. That is, in MRS medium, cultured at 38°C for 22h.

[0038] The formula of the above-mentioned fermentation medium is as follows: soybean peptide 1%, compound fruit and vegetable juice 3%, the balance is raw milk, pH 6.8. Among them, the molecular weight of soybean peptide is 180 Da to 800 Da, and the number of amino acids contained is 2 to 6. The composition of compound fruit and vegetable juice is: longan juice 10%, mango juice 15%, apple juice 30%, pineapple juice 30%, banana juice 5%, burdock juice 5%, carrot juice 5%.

[0039] The base medium is the same as in Example 1.

[0040] The number of live bacteria in the fermented liquid obtained by cultivating method of the present invention and culturing with basal medium is r...

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Abstract

The invention relates to a high-density culture method for lactic acid bacteria. The method comprises the following steps of: activating the lactic acid bacteria in a basic culture medium to obtain two generations; and inoculating the two generations according to the inoculation quantity of 1 percent to a fermentation culture medium to ferment at the temperature of between 35 and 38 DEG C for 20 to 24 hours so as to obtain high-density lactic acid bacteria fermenting liquid, wherein the fermentation culture medium consists of 0.5 to 1.5 percent of soy peptide, 3 to 5 percent of composite fruit and vegetable juice and the balance of raw milk. In the culture method, the raw materials of the fermentation culture medium are easy to obtain; other substances are not added in the culture process, so the method is simple and practicable; the viable bacterium number of the lactic acid bacteria in the fermenting liquid can reach 8.9*10<10> to 2.7*10<11> cfu / mL; and the lactic acid bacteria can serve as base materials of probiotic fermented milk and fermented milk beverage and can be directly used for producing other fermented milk products.

Description

technical field [0001] The invention relates to a high-density culture method for lactic acid bacteria, which belongs to the field of microbial fermentation engineering. Background technique [0002] As beneficial bacteria (or called probiotics) for humans and animals, lactic acid bacteria are widely used in fermented milk products (such as probiotic yogurt, probiotic fermented milk drinks, probiotic cheese), fermented soybean milk products, functional food ingredients and food additives ( Such as in infant formula milk powder, middle-aged and elderly milk powder, fruit and vegetable juice, etc.), dietary supplements (tablets, capsules, etc.) and feed microecological preparations. The production and sales of fermented milk products (mainly yogurt and fermented milk drinks) are growing rapidly, and will continue to maintain this growth trend for a long time to come. Probiotic fermented milk products with certain physiological functions will be fermented milk products A very ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/245C12R1/25
Inventor 魏立华康志远王世杰崔树勇陆淳朱宏冯丽莉卢晓莉李秋琴
Owner JUNLEBAO DAIRY GRP CO LTD
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