Apparatus and method for monitoring cell quantity or cell proportion during dynamic stem cell differentiation

Abstract

The invention discloses an apparatus and a method for monitoring cell quantities or cell proportions during dynamic stem cell differentiation. The invention relates to the field of bioengineering, and especially relates to apparatuses and methods for the on-line or off-line monitoring of cell numbers or other relative information of dynamic cellular differentiation during the cultivation, amplification, and differentiation processes of stem cells. The apparatus comprises: an upstream cell culture solution supplying system or an upstream differentiation solution supplying system; upstream physiological and biochemical index detecting head chambers; detecting heads or connecting heads arranged in the upstream detecting head chambers with an aseptic and sealed condition; cell cultivating apparatuses, downstream physiological and biochemical index detecting head chambers; detecting heads or connecting heads arranged in the downstream detecting head chambers with an aseptic and sealed condition; a fluid speed controlling aseptic pusher; a downstream waste liquid or obtained solution system; and speed-controlling liquid-conveying tubing systems for connecting the above components. According to the method, on-line or off-line monitoring is carried out upon the gas/liquid phase of upstream/downstream cell cultivating system, for monitoring nutrients, metabolites, production concentrations, total quantities, or quantity differences of target cells. Quantities or other relative information of functioning cells or functioning precursor cells differentiated during stem cell differentiation processes can be dynamically obtained.

Description

technical field [0001] The present invention relates to a device and method in the field of bioengineering, in particular to a device and method for online or offline monitoring of the number or number ratio of dynamically differentiated cells and other related information during the process of stem cell culture expansion and differentiation. Background technique [0002] At present, with the deepening of stem cell research, more and more evidences have been found that stem cells have multidirectional differentiation potential. For example, bone marrow mesenchymal stem cells can differentiate into osteoblasts, adipocytes, chondrocytes, nerve cells, etc. These The research results provide a basis for clinical application. However, at present, in most cases, people can only conduct discontinuous, non-dynamic, and result-based identification of the phenomenon of stem cell differentiation, but cannot identify the number of functional cells or their number ratio during the differ...

AI Technical Summary

Problems solved by technology

[0004] Aiming at the current lack of quantitative and dynamic online or offline monitoring methods for the number or number ratio of differentiated cells in the process of stem cell differentiation, the present invention provides a simple and fast online or offline monitoring method based on biochemical principles. Device and method for off-line dynamic quantitative monitoring of differentiated cell number and its ratio and other related information

Images