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Application of indole-3-methanol serving as BRCA1 (breast cancer 1) inducer to preparation of radiation injury protection medicament

A radiation damage and indole technology, which is applied in the field of radiation damage protection drugs, can solve the problem of no radiation damage protection effect of indole-3-carbinol, and achieve the effect of protecting radiation damage.

Inactive Publication Date: 2011-10-05
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the prior art, there is no report on the protective effect of indole-3-carbinol on radiation damage

Method used

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  • Application of indole-3-methanol serving as BRCA1 (breast cancer 1) inducer to preparation of radiation injury protection medicament
  • Application of indole-3-methanol serving as BRCA1 (breast cancer 1) inducer to preparation of radiation injury protection medicament
  • Application of indole-3-methanol serving as BRCA1 (breast cancer 1) inducer to preparation of radiation injury protection medicament

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Cell culture: Culture the cells in D-MEM medium containing 10% calf serum, glutamine, 1 million U / L penicillin and 100 μg / ml streptomycin. Cells were placed in 5% CO 2 , 37 o C culture in the incubator, subculture once every 2-3 days, and take the cells in the logarithmic growth phase for the experiment.

[0024] (2) 184A1 and Hs578Bst cells (6×10 4 / mL) were inoculated into a 96-well plate, and after culturing for 24 hours, different concentrations of indole-3-carbinol were added at a concentration of 0-10 μmol / L, and 6 parallel wells were set for each concentration. After continuing to culture for 24 hours, add 20 μl of MTT (20 μl / ml) to the cell culture medium and continue to culture for 4 hours. Aspirate the culture medium, add 150 μl DMSO to each well, and shake gently on a shaker for 10 minutes. Finally, the optical density was detected by a microplate reader (A570 nm / A630 nm absorbance value), and the cell survival rate was calculated by the method of cel...

Embodiment 2

[0026] (1) cell culture is the same as embodiment one;

[0027] (2) 184A1 and Hs578Bst cells (6×10 4 / mL) were inoculated into 60 mm culture dishes, and the cells were treated with different concentrations (0, 5, 10 μmol / L) of indole-3-carbinol for 24 hours, and then given different doses (0, 2, 4, 6, 8, 10 Gy) of γ-ray irradiation (irradiation conditions: a medical 60 Co γ-ray irradiation source with a dose rate of 0.9 Gy / min). Cells after exposure (1 x 10 4) were inoculated into 60 mm culture dishes, and then cultured continuously for 3 weeks, fixed with methanol, stained with Giemsa application solution for 30 minutes, washed with running water, and counted the number of clones with the number of cells ≥ 50. The colony formation rate and survival fraction were calculated by clone formation rate PE = (clones number in control group / cell number in experimental group) x 100% and survival fraction = clone number / (cell number in experimental group × PE). The result is as f...

Embodiment 3

[0030] SD rats (6 weeks old, about 170g) were raised in a clean animal room with a temperature of 22+ / -2 and a humidity of 60%, and they could freely obtain water and food. The SD rats were randomly divided into two groups : control group and treatment group, 10 in each group; see Figure 4 Shown, wherein, give control group SD rat whole body 13Gy lethal dose irradiation, then observe the death situation of animal continuously for 30 days, the result finds that to the 13th day, control group SD rat all dies; , 50 mg / kg indole-3-carbinol was injected intraperitoneally 24 hours before irradiation, and then SD rats were irradiated with a lethal dose of 13Gy, and then intraperitoneally injected indole-3-carbinol for 7 consecutive days after irradiation, with a total dose of 350 mg / kg, and then observe the death situation of the animals for 30 consecutive days, and it was found that: the animals that received indole-3-carbinol also had 50% of the animals survived after 30 days, an...

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Abstract

The invention belongs to the field of radiation injury protection medicaments and relates to new application of indole-3-methanol serving as a BRCA1 (breast cancer 1) inducer, i.e., the application to the preparation of a radiation injury protection medicament. The structural formula of the indole-3-methanol is shown in the specifications. The indole-3-methanol can be used for preparing the radiation injury protection medicament. By adopting the indole-3-methanol, the survival rates of cells and animals subjected to ionizing radiation are increased at a low concentration with no toxic or side effect.

Description

technical field [0001] The invention belongs to the field of radiation damage protection drugs, and relates to a new application of indole-3-carbinol, that is, an application in preparing radiation damage protection drugs. Background technique [0002] According to the statistics of the World Health Organization, the cure rate of malignant tumors is about 45%, of which surgery accounts for 22%, radiation therapy accounts for 18%, and chemotherapy accounts for 5%. Among them, during the treatment of malignant tumors, about 70-80% of patients need radiation therapy to varying degrees. Some tumors can be cured through radiation therapy, and a considerable number of patients can relieve symptoms and prolong survival through radiation therapy. For example, the five most common malignancies of lung, stomach, liver, colorectum, and esophagus that kill men and the five most common malignancies of breast, lung, stomach, colorectum, and cervix that kill women can be calculated by Rad...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/404A61P39/00
Inventor 樊赛军张慧文汪淼王彦苏任航
Owner SUZHOU UNIV
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