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Preparation method for anti-infectious nano collagen/ calcium phosphate bone repair material

A calcium phosphate bone and repair material technology, applied in medical science, prosthesis, etc., to achieve good strength and biocompatibility, promote adhesion and stretching, and excellent biocompatibility

Inactive Publication Date: 2011-10-05
TSINGHUA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these materials have their own shortcomings, and none of them can compare with autologous bone or allogeneic bone in clinical practice.

Method used

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  • Preparation method for anti-infectious nano collagen/ calcium phosphate bone repair material
  • Preparation method for anti-infectious nano collagen/ calcium phosphate bone repair material
  • Preparation method for anti-infectious nano collagen/ calcium phosphate bone repair material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The materials used are type I collagen gel (concentration: solid content 1%, the collagen contained is purified cowhide collagen), analytically pure CaCl 2 ·6H 2 O. Analytical pure Na 2 HPO 4 , Analytical pure 1,4-dioxane, L-polylactic acid (PLLA) Molecular weight: 100,000.

[0026] (1) Dissolve 20g of collagen gel in 0.5M acetic acid solution to prepare 7.0×10 -4 g / ml collagen solution, slowly add CaCl dropwise to the collagen solution 2 solution, the dropping amount is 0.09 mol of calcium ion per gram of collagen;

[0027] (2) Add Na slowly while stirring in the solution made in step (1). 2 HPO 4 Aqueous solution, added Na 2 HPO 4 with added CaCl 2 The molar ratio is 2:1;

[0028] (3) Slowly add NaOH solution dropwise while stirring in the solution made in step (2) until the pH is 6-8, and the pH value is measured with test paper or a pH meter, and precipitation begins to occur when the pH is 5-6, and the pH value When it was 7, a white suspension appeared;...

Embodiment 2

[0036] The materials used are type I collagen gel (concentration: solid content 1%, the collagen contained is purified cowhide collagen), analytically pure CaCl 2 ·6H 2 O. Analytical pure Na 2 HPO 4 , Analytical pure 1,4-dioxane, L-polylactic acid (PLLA) Molecular weight: 100,000.

[0037] (1) Dissolve 20g of collagen gel in 0.1M hydrochloric acid solution to prepare 5.0×10 -5 g / ml collagen solution, slowly add CaCl dropwise to the collagen solution 2 solution, the dropping amount is 0.01mol of calcium ion per gram of collagen;

[0038] (2) Add Na slowly while stirring in the solution made in step (1). 2 HPO 4 Aqueous solution, added Na 2 HPO 4 with added CaCl 2 The molar ratio is 2:1;

[0039] (3) Slowly add NaOH solution dropwise while stirring in the solution made in step (2) until the pH is 6-8, and the pH value is measured with test paper or a pH meter, and precipitation begins to occur when the pH is 5-6, and the pH value When it was 7, a white suspension appe...

Embodiment 3

[0047] The materials used are type I collagen gel (concentration: solid content 1%, the collagen contained is purified cowhide collagen), analytically pure CaCl 2 ·6H 2 O. Analytical pure Na 2 HPO 4 , Analytical pure 1,4-dioxane, L-polylactic acid (PLLA) Molecular weight: 100,000.

[0048] (1) Dissolve 20g of collagen gel in 0.2M nitric acid solution to prepare 5.0×10 -3 g / ml collagen solution, slowly add CaCl dropwise to the collagen solution 2 solution, the dropping amount is 0.16 mol of calcium ion per gram of collagen;

[0049] (2) Add Na slowly while stirring in the solution made in step (1). 2 HPO 4 Aqueous solution, added Na 2 HPO 4 with added CaCl 2 The molar ratio is 2:1;

[0050] (3) Slowly add NaOH solution dropwise while stirring in the solution made in step (2) until the pH is 6-8, and the pH value is measured with test paper or a pH meter, and precipitation begins to occur when the pH is 5-6, and the pH value When it was 7, a white suspension appeared;...

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Abstract

The invention discloses a preparation method for an anti-infectious nano collagen / calcium phosphate bone repair material, and belongs to the field of biomedical materials. The method comprises the following steps of: slowly dripping solution containing calcium ions and aqueous solution containing phosphate radical ions into acid-soluble collagen solution, dripping NaOH solution to regulate the pH value, performing freeze drying, grinding to obtain dry powder for later use, dissolving poly-L-lactic acid in 1,4-dioxane, stirring for a certain time, mixing the solution and the dry powder uniformly, finally adding vancomycin, stirring uniformly, removing the solvent by adopting a freeze drying method, and preparing the anti-infectious nano collagen / calcium phosphate bone repair material. The prepared bone repair material has excellent biocompatibility, infection resistance and a micro-structure similar to human body.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and relates to a nano-collagen / calcium phosphate bone repair material with anti-infection effect and a preparation method thereof. Background technique [0002] According to the report of AO organization, about 5% of the people using internal fixation have infection, and the average cost of treatment for each infection is 15,000 US dollars. Against the background of the high incidence of fixture-associated infections, the increasing number of antibiotic-resistant strains, and the growing recognition of the limitations of systemic antibiotics, topical prophylactic or therapeutic antibiotics have become promising in the treatment of fixture-associated infections. One of the main measures, especially the way of combining or synthesizing fixatives with antibiotics. The local drug delivery system of compound antibiotics releases antibiotics locally, continuously and at high concentrations to the i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/54A61L27/56A61L27/44A61L27/12A61L27/24
Inventor 连小洁王秀梅胡堃崔福斋郭文广胡艳丽
Owner TSINGHUA UNIV
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