Method for preparing maize germ oil through enzymatic degumming
A corn germ oil and enzymatic technology, which is applied in the direction of edible oil/fat, fat oil/fat refining, and fat production, can solve the problems of difficult oil extraction, difficult to grasp the refining stability, etc., and achieves consumption saving and low phospholipid content. , cost-saving effect
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[0048] Example 1.
[0049] Optimal test of enzymatic degumming process data in corn germ oil refining process
[0050] Test plan: Through single factor test on the type of acid, the amount of acid added, the amount of enzyme added, etc., the optimal reaction conditions are explored.
[0051] Materials and methods 1.1 Raw materials and reagents Crude corn oil: self-produced, initial phosphorus content is 439mg / kg, free fatty acid content is 3.67%; Lecitase Ultra (EC3.1.1.3): A1-position phospholipase, produced by microbial fermentation, Novozyme Company Gift; other reagents: analytical grade. 1.2 Main instruments FJ-200 high-speed homogenizer, 752 spectrophotometer, electric heating constant temperature water bath, JB90-D powerful electric mixer, etc. 1.3 Phosphorus content analysis Take about 10mL of oil sample, in order to facilitate the separation of oil and gum, first heat it in a water bath to 80°C, hold it for 10 minutes and centrifuge at 5000 rpm for 10 minutes, and take 5g u...
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[0075] Example 2
[0076] Optimal test parameters for industrialized operation
[0077] ⑴ Acid reaction.
[0078] The crude oil (flow rate 8.3 m3 / h) is heated to 55°C through the heat exchanger,
[0079] The pump flow rate of citric acid (50% solution) is 13.55 liters / hour;
[0080] Preparation of citric acid solution: add 50 kg of citric acid to 50 kg of hot soft water at 55℃ to fully dissolve;
[0081] Maintain at 55°C for more than 30 minutes in the acid reaction tank.
[0082] ⑵ Add alkali: 16% NaOH solution, control PH 5~5.3; alkali solution flow rate is about 12.5 liters / hour;
[0083] ⑶ Enzyme addition: the flow rate of enzyme solution is 20.472 liters / hour; the amount of enzyme addition is 75PPM;
[0084] Enzyme solution preparation: add 5.66 kg of enzyme to 200 kg of cold soft water.
[0085] ⑷ Add water: flow rate 105 liters / hour;
[0086] ⑸ High-speed mixing. After adding the enzyme preparation, the grease enters the enzyme reaction tank through a high-speed shear mixer. High-spe...
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