Method for purifying salvianolic acid A by adopting normal phase chromatography

Abstract

The invention provides a method for purifying salvianolic acid A by adopting a normal phase chromatography based on physicochemical properties, chromatographic behaviors and unit separation technology adaptabilities of chemical compositions of salvia miltiorrhiza. A normal phase chromatographic packing is chromatographic silica gel the granularity of which is 100-200 meshes, wherein the normal phase chromatography comprises the following operation steps of: extracting a salvianolic acid A aqueous solution by using ethyl acetate; concentrating ethyl acetate extract liquor until the solid content is 20-30 percent; adding chromatographic silica gel in an amount which is 2-2.5 times that of the salvianolic acid A; putting into a rotatory evaporator for drying at reduced pressure to obtain a flowing solid sample; and putting the solid sample at the top end of a silica gel wet column. A produced prepared with the method has stable and controllable quality and acceptable cost.

Description

technical field [0001] The invention relates to a method for purifying salvianolic acid A by normal phase chromatography. Background technique [0002] Salvia miltiorrhiza is the basic treatment drug for cardiovascular and cerebrovascular diseases in my country. Due to its definite curative effect, Danshen has become the traditional Chinese medicine with the largest consumption (15,000 tons / year), the highest sales, and the largest number of preparation factories in China (960 preparation factories, and the main dosage forms are: Danshen Dropping Pills, Danshen Injection, Danshen Tablets, Danshen Granules, Danshen Granules and Danshen Capsules), in addition, Danshen is also compounded with other medicinal materials for pulmonary heart disease, hyperlipidemia, hyperviscosity syndrome, asthma, bronchopneumonia, Hepatitis, liver cirrhosis, nephrotic syndrome, arthritis, diabetes, circulatory disorders, various bacterial and viral infections, anti-endotoxin, tumor, scleroderma, de...

AI Technical Summary

Problems solved by technology

[0010] Although membrane separation technology can effectively remove pseudo-colloidal impurities, because it is a non-tangential force separation technology, the target compound often has a very high affinity with this type of impurity, resulting in serious loss of the target compound

In addition, when membrane technology is used for the separation of such impurities, it also faces the problems of membrane pore clogging and membrane life.

[0011] Solvent selectivity in solvent extraction techniques is critical and the effect is not ideal

[0012] Traditional C 18 Reversed-phase chromatography cannot be widely used due to factors such as small sample volume, limited pH range, inability to perform effective regeneration, short life, and cost constraints.

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