Polymerase chained type reactor and real-time micro-optics detection device

Abstract

The invention discloses a polymerase chained type reactor and a real-time micro-optics detection device thereof, relating to the field of biochemical reaction and medicine detection. The real-time micro-optics detection device comprises a basal layer with a helical micro-channel structure, and the basal layer is sealed with a cover plate layer. Semiconductor heating refrigerators are arranged below a microfluidic chip; and a microprocessor is used for controlling the three semiconductor heating refrigerators to ensure that a whole micro-channel area is divided into a denaturation area, an annealing area and an extending area which are required for polymerase chained type reaction. Three platinum resistor temperature sensors are arranged between the microfluidic control chip and the three semiconductor heating refrigerators to form a closed-loop temperature control. A real-time fluorescence detection system comprises a light source, a photoelectric detector and a fluorescent signal acquiring and processing system, wherein a linear array light-emitting diode is used as the light source, and a photoelectric photomultiplier is used as the photoelectric detector; and the linear array light-emitting diode and the photoelectric photomultiplier are fixed above the extending area of the microfluidic control chip by an optical stand. According to the polymerase chained type reactor and the real-time micro-optics detection device disclosed by the invention, temperature rise and fall time is omitted, the time of polymerase chained type reaction is shortened and the service life of thesemiconductor heating refrigerators is prolonged.

Description

Technical field [0001] The invention relates to polymerase chain reaction (Polymerase Chain Reaction, PCR) to realize DNA amplification and real-time fluorescence detection on a microfluidic chip. Used in the fields of biochemical reaction and medical testing. Background technique [0002] Miniaturization is an important development direction in medical and biochemical testing equipment, and the development of Micro-electro-mechanical systems (MEMS) technology has made it possible for PCR reactions to be completed on a chip. The traditional fluorescent PCR instrument has the following shortcomings: the instrument is large, the temperature rise and fall rate is slow, the dynamic temperature control accuracy is not high, and the long-term temperature sudden change of the semiconductor heating refrigerator shortens the life. Now there are three types of PCR reaction chips manufactured by MEMS technology: micro-well chips, micro-fluidic chips and micro-water bath chips. The micro-w...

AI Technical Summary

Problems solved by technology

The traditional fluorescent PCR instrument has the following disadvantages: the instrument is bulky, the heating and cooling rate is slow, the dynamic temperature control accuracy is not high, and the long-term temperature sudden change of the semiconductor heating refrigerator shortens the service life

The micro-well chip has the following disadvantages: slow heating and cooling rate, dynamic temperature control, and low temperature accuracy. Generally, there are only alloy layer micro-heaters and no active refrigerators, such as patents 200310122607.9 and 200510011180.4, etc.

Microfluidic chips have the following disadvantages: Generally, there are only alloy layer micro heaters, and no active coolers, such as patent 200410024703.4, etc.

Micro water bath chip 200610043243.9, etc., with complex heating and cooling structures

The PCR reaction chip manufactured by using MEMS technology solves the problem of large volume of the instrument, but there is also the problem of integrating active cooling and real-time fluorescence detection on the chip

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