Medium and cultivating method for Tibetan mushroom rapid propagation
The technology of a Tibetan spirit mushroom and its cultivation method is applied in the medium and cultivation field of the rapid proliferation of Tibetan spirit mushrooms, which can solve the problems of flavor and quality changes of fermented dairy products, restrictions on industrial production, and bacterial phase imbalance, and achieve fast growth, Easy storage and transportation, short cycle effect
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Embodiment 1
[0023] The medium formula is: tomato juice 5 mL, carrot juice 5 mL, soybean powder 2 g, and milk 90 mL.
[0024] 1. Culture medium preparation
[0025] The preparation method of the culture medium is as follows: add 5 mL of tomato juice, 5 mL of carrot juice, 2 g of soybean powder, and 90 mL of milk into a 250 mL Erlenmeyer flask, adjust the initial pH value to 6.2, and put it in a high-pressure steam sterilizer at 115 °C Sterilize for 15 min.
[0026] The preparation method of the tomato juice used is: weigh 150 g tomatoes, crush them in a tissue grinder, filter them with an 80-mesh filter cloth, and take the filtrate.
[0027] The preparation method of the carrot juice used is: weigh 150 g of carrots, add 150 mL of sterile water, pulverize it in a tissue grinder, and filter it with an 80-mesh filter cloth.
[0028] The method for preparing the soybean powder is as follows: put a certain amount of dry soybeans into a tissue grinder and pulverize them to obtain the soybean p...
Embodiment 2
[0038] The formula of the medium is: tomato juice 10 mL, carrot juice 10 mL, soybean powder 5 g, milk 80 mL.
[0039] 1. Culture medium preparation
[0040] The preparation method of the culture medium is as follows: add 10 mL of tomato juice, 10 mL of carrot juice, 5 g of soybean flour, and 80 mL of milk into a 250 mL Erlenmeyer flask, adjust the initial pH value to 6.5, and put it in a high-pressure steam sterilizer at 115 °C Sterilize for 15 min.
[0041] The preparation method of the tomato juice used is: weigh 150 g tomatoes, crush them in a tissue grinder, filter them with an 80-mesh filter cloth, and take the filtrate.
[0042] The preparation method of the carrot juice used is: weigh 150 g of carrots, add 150 mL of sterile water, pulverize it in a tissue grinder, and filter it with an 80-mesh filter cloth.
[0043] The method for preparing the soybean powder is as follows: put a certain amount of soybeans into a tissue grinder and pulverize them to obtain the soybean...
Embodiment 3
[0053] The medium formula is: tomato juice 20 mL, carrot juice 20 mL, soybean powder 10 g, milk 60 mL.
[0054] 1. Culture medium preparation
[0055] The preparation method of the culture medium is: add 20 mL of tomato juice, 20 mL of carrot juice, 10 g of soybean powder, and 60 mL of milk into a 250 mL Erlenmeyer flask, adjust the initial pH value to 6.2, and put it in a high-pressure steam sterilizer at 115 °C Sterilize for 15 minutes.
[0056] The preparation method of the tomato juice used is: weigh 150 g tomatoes, crush them in a tissue grinder, filter them with an 80-mesh filter cloth, and take the filtrate.
[0057] The preparation method of the carrot juice used is: weigh 150 g of carrots, add 150 mL of sterile water, pulverize it in a tissue grinder, and filter it with an 80-mesh filter cloth.
[0058] The method for preparing the soybean powder is as follows: put a certain amount of dry soybeans into a tissue grinder and pulverize them to obtain the soybean powder...
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