Microbe starter culture for producing conjugated linoleic acid and preparation method thereof

Abstract

The invention provides a microbe starter culture for producing conjugated linoleic acid and a preparation method thereof. The preparation method comprises the following steps that: 1) lactobacillus plantarum CICC 23138 as an original strain is activated through 0.9% of normal saline and is set aside for later use; 2) linoleic acid is added into a lactobacillus plantarum basic medium and the original strain activated in the step 1 is inoculated into the lactobacillus plantarum basic medium, wherein the volume of the added linoleic acid accounts for 0.1% of the volume of the lactobacillus plantarum basic medium and the volume of the added original strain accounts for 5% of the volume of the lactobacillus plantarum basic medium; the inoculated original strain is putted into an anaerobic incubator to be cultured statically for 30 hours at a temperature of 37 DEG C to form cultured bacterial strain as a grade 1 acclimated bacterial strain; linoleic acid is added into a lactobacillus plantarum basic medium and the grade 1 acclimated bacterial strain is inoculated into the lactobacillus plantarum basic medium, wherein the volume of the added linoleic acid accounts for 0.2% of the volume of the lactobacillus plantarum basic medium and the volume of the added grade 1 acclimated bacterial strain accounts for 5% of the volume of the lactobacillus plantarum basic medium; the inoculated grade 1 acclimated bacterial strain is putted into an anaerobic incubator to be cultured statically for 30 hours at a temperature of 37 DEG C to form cultured bacterial strain as a grade 2 acclimated bacterial strain; in the same way, acclimating processes are carried out grade by grade and a grade 7 acclimated bacterial strain is obtained after a grade 7 acclimating process is finished, wherein a low grade acclimated bacterial strain is utilized as an original strain of a higher grade acclimating process and an additive volume of linoleic acid is increased by 0.1% of the volume of a lactobacillus plantarum basic medium; 3) the grade 7 acclimated bacterial strain is cultured in defatted milk and a mature fermented strain is obtained when the defatted milk contains equal to or greater than 1010 living bacteria per milliliter; and 4) the mature fermented strain is treated through the processes of freezing and drying to form culture starter powder.

Description

(1) Technical field [0001] The invention relates to a microbial starter for producing conjugated linoleic acid and a preparation method thereof, in particular to a starter for preparing conjugated linoleic acid by fermenting linoleic acid and a preparation method thereof, belonging to the field of bioengineering. (2) Background technology [0002] Conjugated linoleic acid (CLA) is a class of octadecadienoic acid containing cis- or trans-conjugated double bonds. It is one of the most important functional fatty acids discovered by humans in recent years. CLA has various physiological activities such as anti-cancer, weight loss, anti-atherosclerosis, prevention and treatment of diabetes, regulation of blood lipids, enhancement of body immunity and growth promotion. c9, t11 CLA and t10, c12 CLA are the most physiologically active. However, the source of CLA is very limited, only in milk and meat products of ruminants, and the content of non-ruminant products is very low. [00...

AI Technical Summary

Problems solved by technology

However, the source of CLA is very limited, only in milk and meat products of ruminants, and the content of non-ruminant products is very low

[0003] At present, the industrially produced CLA product is a mixture of multiple isomers, with poor selectivity, difficult quality assurance, and questioned safety

However, the use of microbial transformation to produce CLA has mild reaction conditions and a relatively single isomer composition, which is conducive to product development and application.

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