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Method for producing carotinoid through cordyceps solid culture

A technology of carotene and solid culture, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of low extraction rate of mycelium carotenoids, achieve low price, high yield, Source Rich Effects

Inactive Publication Date: 2011-11-09
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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Problems solved by technology

[0005] At present, there are few studies on carotenoids produced by Cordyceps militaris. In 2006, Fu Mingjia applied for a patent "Process for producing carotenoids by fermentation from Cordyceps militaris" (Chinese patent application number: 200610018266.4). Carry out liquid static fermentation, collect the mycelium on the surface of the fermentation broth and extract it with ethanol, which has the disadvantages of low mycelium yield and carotenoid extraction rate

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  • Method for producing carotinoid through cordyceps solid culture

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Embodiment 1

[0023] Embodiment 1, the method for extracting carotenoids from the fruiting bodies of Cordyceps militaris solid culture

[0024] (1) Strain activation

[0025] Cordyceps militaris 1065 (preservation number: CGMCC 5.1973) was inoculated on potato solid medium (PDA) for activation, the culture temperature was 20° C., and the culture time was 10 days.

[0026] (2) Strain cultivation: with the mycelia of step (1), inoculate under aseptic conditions into PDA liquid culture medium (medium consists of: potato 200g boiled juice, glucose 20g, constant volume to 1000ml), culture temperature At 20°C, the culture method is to use a vortex shaker to cultivate at a rotation speed of 150 rpm, and cultivate to the logarithmic growth phase.

[0027] (3) Solid culture: insert the liquid strain activated in step (2) into the rice culture medium, and place it in an incubator for cultivation. First, under the condition of a temperature of 20° C., cultured in the dark for 10 days; then irradiate...

Embodiment 2

[0029] Embodiment 2, the method for extracting carotenoids from Cordyceps militaris solid medium residue

[0030] (1) Strain activation

[0031] Cordyceps militaris 1065 (preservation number: CGMCC 5.1973) was inoculated on potato solid medium (PDA) for activation, the culture temperature was 20° C., and the culture time was 10 days.

[0032] (2) Strain cultivation: use the mycelium of step (1) to inoculate in the potato liquid culture medium under aseptic conditions, the cultivation temperature is 20° C., and the cultivation method is to utilize a vortex shaker to cultivate, and the rotating speed is 150 rpm , cultivated to the logarithmic growth phase.

[0033] (3) Solid culture: insert the liquid seed activated in step (2) into the rice culture medium, and place it in an incubator for cultivation. First, culture at 18 DEG C in the dark for 10 days, then irradiate with natural light, culture at 18-25 DEG C for 40 days, and harvest the fruiting body of Cordyceps militaris t...

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Abstract

The invention discloses a method for producing carotinoid through cordyceps solid culture. The method comprises the following steps: 1) carrying out a solid culture for cordyceps militaris, and collecting culture products; 2) carrying out a wall-breaking process for the culture products, followed by centrifugating and collecting precipitation; 3) carrying out extracting for the precipitation through a organic solvent, followed by filtering to obtain carotinoid crude extracting solution, then removing the organic solvent from the carotinoid crude extracting solution to obtain the carotinoid. With the method provided by the present invention, yield of the carotinoid is high, wherein 60-700 [mu]g carotinoid can be obtained through extracting one gram of fresh culture products; the prepared cordyceps carotinoid presents bright orange yellow, and is a natural product. In addition, culture media raw materials required by the solid culture provided by the present invention have characteristics of abundant source and low price; the method does not require special equipment; culture media residues generated during producing are developed and used.

Description

technical field [0001] The invention relates to a method for extracting carotenoids from products of solid culture of Cordyceps militaris. Background technique [0002] Today, food safety is attracting more and more attention. Natural food coloring is more and more valued by people because of its natural color, safety and non-toxicity. Natural carotenoids (Carotenoids) are terpenoids composed of eight isoprenes, covering the range from bright yellow to dark red. In addition to adding color to food, it also has high nutritional and health value. Almost all carotenoids have the functions of anti-oxidation, enhancing immunity, preventing ultraviolet damage, anti-cardiovascular disease and anti-cancer. β-carotene and α-carotene are also the precursors of vitamin D. As food additives and nutritional supplements, carotenoids have been recognized by international organizations such as the US Food and Drug Administration, the European Community, and the World Health Organization. ...

Claims

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Application Information

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IPC IPC(8): C12P23/00C12R1/645
Inventor 姚一建董彩虹
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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