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Semi-continuous fermentation method for bacillus used for microbial fertilizer

A technology of microbial fertilizer and bacillus, applied in the field of microbial culture, can solve the problems of low utilization rate of equipment, high requirements for aseptic operation, difficult production control, etc., so as to shorten the production cycle, reduce the number of expansions, and facilitate production control. Effect

Active Publication Date: 2011-11-16
江苏科邦生态肥有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Each batch of cultivation requires seed expansion, resulting in a long production cycle, low utilization of equipment, and high requirements for aseptic operation
Batch operation also brings difficulties to production control

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The culture medium of Bacillus subtilis (by weight percentage, wheat flour 2%, corn steep liquor 0.2%, dipotassium hydrogen phosphate 0.5%, soybean cake powder 0.5%, rapeseed oil 0.2%, pH7.2-7.4, the rest is water ) 700 kg was added to the fermenter 1, stirred evenly, steam was passed into the jacket, the temperature of the medium was raised to 121°C, the gauge pressure was 0.11Mpa, and the temperature was maintained for 25 minutes for sterilization. Cool down to 40°C, add 70 kg of Bacillus subtilis seed solution into the fermenter 1 through the inoculation pipeline, at a tank temperature of 37°C, a tank pressure of 0.02-0.05Mpa, a stirring speed of 320 rpm, and an air flow of 1:1.2 volume / Volume / minute is cultivated, cultivated for 36 hours, microscopic examination culture begins to appear spores, and 500 kg of fermentation liquid is transported to fermenter 2 through pipeline. Fermentation tank 2 was cultured at a tank temperature of 37°C, a tank pressure of 0.02-0.0...

Embodiment 2

[0026] The culture medium of Bacillus colloidus (by weight percentage, wheat flour 1%, soybean cake powder 0.5%, dipotassium hydrogen phosphate 0.05%, magnesium sulfate 0.25%, ammonium sulfate 0.1%, foam enemy 0.002%, pH7.2- 7.4, the rest is water) 700 kg was added to the fermenter 1, stirred evenly, steam was introduced into the jacket, the temperature of the medium was raised to 121°C, the gauge pressure was 0.11Mpa, and the temperature was maintained for 25 minutes for sterilization. Cool down to 40°C, add 100 kg of Bacillus colioids seed solution into the fermenter 1 through the inoculation pipe, at a tank temperature of 35±2°C, a tank pressure of 0.05Mpa, a stirring speed of 280 rpm, and a ventilation rate of 1:1.0 volume Cultivate / volume / min, cultivate for 40 hours, microscopic examination culture begins to appear spores, and transport 400 kilograms of fermented liquid to fermentor 2 through pipeline. Fermentation tank 2 was cultured at a tank temperature of 37°C, a tank...

Embodiment 3

[0030]The culture medium of Bacillus licheniformis (by weight percentage, wheat flour 1%, corn flour 1.0%, soybean meal 2.5%, corn steep liquor 0.3%, magnesium sulfate heptahydrate 0.2%, manganese sulfate monohydrate 0.04%, rapeseed oil 0.2%, pH7.2-7.4, the rest is water) 700 kg was added to the fermenter 1, stirred evenly, the steam was passed into the jacket, the temperature of the culture medium was raised to 121°C, the gauge pressure was 0.11Mpa, and the temperature was kept for 25 minutes to sterilize. bacteria. Cool down to 40°C, add 70 kg of Bacillus licheniformis seed solution into the fermenter 1 through the inoculation pipeline, at a tank temperature of 37°C, a tank pressure of 0.05Mpa, a stirring speed of 300 rpm, and a ventilation rate of 1:1.0 volume / volume / Cultivated separately, cultivated for 36 hours, microscopic examination culture began to appear spores, and 500 kg of fermented liquid was transported to fermenter 2 by pipeline. Fermentation tank 2 was cultu...

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Abstract

The invention relates to a semi-continuous fermentation method for bacillus used for a microbial fertilizer. The method comprises the following steps of: preparing a bacillus fermentation liquor by suing a two-step culture method; culturing the fermentation liquor until a late stage of a log phase or an initial stage of a stable phase, flowing partial fermentation liquor into another fermentationtank, and culturing into mature fermentation liquor; and performing fed batch culture on the original fermentation tank. The fed batch times can be 16 to 20 times, the times of expanding culture of seeds is greatly reduced, the production period is shortened, the production efficiency is improved, and the semi-continuous industrial production can be realized.

Description

technical field [0001] The invention belongs to the technical field of microbial cultivation, relates to a fermentation method, and specifically designs a semi-continuous fermentation method for bacillus liquid used in microbial fertilizers. Background technique [0002] Over the years, chemical fertilizers have played an important role in increasing agricultural production and income, but their damage to the environment has attracted widespread attention. Microbial fertilizers play an important role in improving soil nutritional structure, enhancing soil fertility, promoting crop growth, and enhancing crop disease resistance. Microbial fertilizers have become the focus of fertilizer research in various countries because of their advantages such as non-toxic, harmless, significant and long-lasting fertilizer effects, convenient application, and wide application range. The Ministry of Agriculture of the People's Republic of China has promulgated a series of standards for mic...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08C12R1/125C12R1/11C12R1/10C12R1/07
Inventor 杨柳唐旺全杨红兵
Owner 江苏科邦生态肥有限公司
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