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Semi-continuous fermentation method for bacillus used for microbial fertilizer

A microbial fertilizer and bacillus technology, applied in the field of microbial cultivation, can solve the problems of low utilization rate of equipment, high requirements for aseptic operation, difficult production control, etc., to shorten the production cycle, reduce the number of expansion cultures, and facilitate production control Effect

Active Publication Date: 2013-08-28
江苏科邦生态肥有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Each batch of cultivation requires seed expansion, resulting in a long production cycle, low utilization of equipment, and high requirements for aseptic operation
Batch operation also brings difficulties to production control

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The culture medium of Bacillus subtilis (by weight percentage, wheat flour 2%, corn steep liquor 0.2%, dipotassium hydrogen phosphate 0.5%, soybean cake powder 0.5%, rapeseed oil 0.2%, pH7.2-7.4, the rest is water ) 700 kg was added to the fermenter 1, stirred evenly, steam was passed into the jacket, the temperature of the medium was raised to 121°C, the gauge pressure was 0.11Mpa, and the temperature was maintained for 25 minutes for sterilization. Cool down to 40°C, add 70 kg of Bacillus subtilis seed solution into the fermenter 1 through the inoculation pipeline, at a tank temperature of 37°C, a tank pressure of 0.02-0.05Mpa, a stirring speed of 320 rpm, and an air flow of 1:1.2 volume / Volume / minute is cultivated, cultivated for 36 hours, microscopic examination culture begins to appear spores, and 500 kg of fermentation liquid is transported to fermenter 2 through pipeline. Fermentation tank 2 was cultured at a tank temperature of 37°C, a tank pressure of 0.02-0.0...

Embodiment 2

[0026] The culture medium of Bacillus colloidus (by weight percentage, wheat flour 1%, soybean cake powder 0.5%, dipotassium hydrogen phosphate 0.05%, magnesium sulfate 0.25%, ammonium sulfate 0.1%, foam enemy 0.002%, pH7.2- 7.4, the rest is water) 700 kg was added to the fermenter 1, stirred evenly, steam was introduced into the jacket, the temperature of the medium was raised to 121°C, the gauge pressure was 0.11Mpa, and the temperature was maintained for 25 minutes for sterilization. Cool down to 40°C, add 100 kg of Bacillus colioids seed solution into the fermenter 1 through the inoculation pipe, at a tank temperature of 35±2°C, a tank pressure of 0.05Mpa, a stirring speed of 280 rpm, and a ventilation rate of 1:1.0 volume Cultivate / volume / min, cultivate for 40 hours, microscopic examination culture begins to appear spores, and transport 400 kilograms of fermented liquid to fermentor 2 through pipeline. Fermentation tank 2 was cultured at a tank temperature of 37°C, a tank...

Embodiment 3

[0030]The culture medium of Bacillus licheniformis (by weight percentage, wheat flour 1%, corn flour 1.0%, soybean meal 2.5%, corn steep liquor 0.3%, magnesium sulfate heptahydrate 0.2%, manganese sulfate monohydrate 0.04%, rapeseed oil 0.2%, pH7.2-7.4, the rest is water) 700 kg was added to the fermenter 1, stirred evenly, the steam was passed into the jacket, the temperature of the culture medium was raised to 121°C, the gauge pressure was 0.11Mpa, and the temperature was kept for 25 minutes to sterilize. bacteria. Cool down to 40°C, add 70 kg of Bacillus licheniformis seed solution into the fermenter 1 through the inoculation pipeline, at a tank temperature of 37°C, a tank pressure of 0.05Mpa, a stirring speed of 300 rpm, and a ventilation rate of 1:1.0 volume / volume / Cultivated separately, cultivated for 36 hours, microscopic examination culture began to appear spores, and 500 kg of fermented liquid was transported to fermenter 2 by pipeline. Fermentation tank 2 was cultu...

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Abstract

The invention relates to a semi-continuous fermentation method of a bacillus liquid used for microbial fertilizers. The bacillus fermentation liquid is prepared by a two-step culture method. The fermentation broth is cultivated to the end of the logarithmic phase or the beginning of the stationary phase, and part of the fermentation broth is flowed out to another fermenter for cultivation until the mature fermentation broth is cultivated, and the original fermenter is fed for culture. Feeding can be carried out 16-20 times, which greatly reduces the number of seed expansion, shortens the production cycle, improves production efficiency, and can realize semi-continuous industrial production.

Description

technical field [0001] The invention belongs to the technical field of microbial cultivation, relates to a fermentation method, and specifically designs a semi-continuous fermentation method for bacillus liquid used in microbial fertilizers. Background technique [0002] Over the years, chemical fertilizers have played an important role in increasing agricultural production and income, but their damage to the environment has attracted widespread attention. Microbial fertilizers play an important role in improving soil nutritional structure, enhancing soil fertility, promoting crop growth, and enhancing crop disease resistance. Microbial fertilizers have become the focus of fertilizer research in various countries because of their advantages such as non-toxic, harmless, significant and long-lasting fertilizer effects, convenient application, and wide application range. The Ministry of Agriculture of the People's Republic of China has promulgated a series of standards for mic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/125C12R1/11C12R1/10C12R1/07
Inventor 杨柳唐旺全杨红兵
Owner 江苏科邦生态肥有限公司
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