Method for preparing beta-glucanase for feeding by utilizing solid-state fermentation of peanut hull meal

Abstract

The invention relates to a method for preparing beta-glucanase for feeding by utilizing solid-state fermentation of peanut hull meal, which can effectively achieve the preparation of the beta-glucanase for feeding to meet the requirements of livestock breeding for feed production. The preparation method comprises the following steps of: (A) preparation of strain: firstly activating the strain, and then carrying out primary seed culture and secondary seed culture; and (B) preparation of culture medium (beta-glucanase for feeding): firstly preparing a fermentation medium, then cooling, inoculating secondary seeds cultured in the step (A), transferring the secondary seeds into a fermentation bed for culturing, fermenting to obtain a fermentation product which is flaxen or beige and has natural flavor, and then baking, crushing and subpackaging. In the invention, the fermentation medium takes common agricultural and sideline products including peanut hull meal, bran and the like as main raw materials; the culture is convenient, the growth rate is high, and the fermentation period is greatly shortened (only 37 hours); a beta-glucanase preparation for feeding can be produced; the beta-glucanase for feeding has the advantages of high enzyme activity, good heat resistance and good application effect; and a microbial strain (aspergillus niger) is used for fermenting, thus the safety is high, and harmful substances can not be produced.

Description

1. Technical field [0001] The invention relates to biological fermentation, in particular to a method for preparing feed beta-glucanase by solid-state fermentation of peanut shell powder. 2. Background technology [0002] Glucan is a non-starch polysaccharide, which is a polysaccharide connected by β-1,3 glucosidic bonds and β-1,4 glycosidic bonds. It exists in the aleurone layer and endosperm plant cell wall of barley, oats, wheat, etc. Among them, it has high viscosity in aqueous solution. Because monogastric animals do not have microorganisms that synthesize β-glucanase and enzyme systems that decompose glucan, the chyme has a high viscosity in the intestine, which prevents the absorption of protein and fat nutrients, and shows strong The anti-nutritional effect of feed reduces the conversion rate of feed, inhibits the growth of livestock and poultry, and finally leads to the increase of animal output cost. Feed β-glucanase is used in the feed industry to degrade the an...

AI Technical Summary

Problems solved by technology

For the production of enzyme preparations for feed, foreign companies mostly use a single-species pure-bred liquid fermentation process to produce a single enzyme preparation. This method requires a lot of investment and high enzyme costs. Due to the low added value of my country's feed industry products, it is not suitable for the current situation of my country's feed breeding industry.

Therefore, it is difficult to effectively promote and apply, and cannot meet the needs of the aquaculture industry for feed production, and its innovation is imperative

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