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Method for preparing indigo naturalis

A technology of Indigo Naturalis and plants, applied in the field of medicine, can solve the problems of uneven quality of Indigo Naturalis finished products, difficult to standardize the processing process, affecting clinical efficacy and other problems, and achieve the effects of good product quality, reduction of lime dosage, and expansion of clinical application scope.

Inactive Publication Date: 2011-11-23
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current research on the processing method of Indigo Naturalis only uses indigo as an index to control product quality. How to increase the content of indirubin or indigo in Indigo Naturalis is worth studying
In addition, the pH adjusted during the indigo production process is a key factor affecting the production of indigo and indirubin, and the actual dosage of milk of lime is entirely based on experience, which makes it difficult to standardize the processing technology, resulting in uneven quality of Indigo Naturalis finished products, affecting clinical efficacy play

Method used

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  • Method for preparing indigo naturalis
  • Method for preparing indigo naturalis
  • Method for preparing indigo naturalis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1 Preparation of crude indigo of the present invention

[0014] Place the fresh leaves in tap water at 25°C with a pH of about 3.5, where the volume of water: the mass of fresh leaves (ml:g) is 13. After soaking in the dark for 24 hours, filter to remove the leaf residue, add ammonia water to adjust the pH of the filtrate to 9, and then After ventilating for 30 minutes, add 10% freshly made lime milk (take 10% CaO of fresh leaf mass, add 10 times the amount of boiling water, keep stirring to form a suspension, that’s it), stir well and let stand for 24h, filter Take the precipitate and dry to obtain crude indigo.

Embodiment 2

[0015] Example 2 Preparation of crude indigo of the present invention

[0016] Place the fresh leaves in tap water at 60°C with a pH of about 7, where the volume of water: the mass of fresh leaves (ml:g) is 15. After soaking for 48 hours, filter to remove the leaf residue, let the filtrate pass air for 30 minutes, and add ammonia to adjust the pH of the filtrate to 10.5. Add 10% freshly made lime milk (take 10% CaO of fresh leaf mass, add 10 times the amount of boiling water, keep stirring to form a suspension after 180 minutes), stir evenly and then stand still After 24h, filter the precipitate and dry to obtain crude indigo.

Embodiment 3

[0017] Example 3 Selection of process parameters for directional formation of indigo during crude indigo processing

[0018] 1. Instruments and reagents

[0019] Shimadzu LC-10ATvp high performance liquid chromatograph (Zhejiang Zhida N2000 Chromatography Workstation); BP211D electronic analytical balance (Max=210g, d=0.01mg, Germany Sartorius); KQ-400DB CNC ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); SZ-93 automatic double pure water distiller (Shanghai Yarong Biochemical Instrument Factory); LZB-FB rotameter (Suzhou Chemical Instrument Co., Ltd.); ACO series electromagnetic air compressor (Zhejiang Sensen Industrial Co., Ltd.); SHB-ⅢS circulating water multi-purpose vacuum pump (Zhengzhou Great Wall Technology Industry and Trade Co., Ltd.)

[0020] Malan stems and leaves (provided by Sichuan Jiangyou Hengyuan Pharmaceutical Co., Ltd.); Indigo (China Institute for the Control of Pharmaceutical and Biological Products, batch number: 110716-200509); Indirubin (China...

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Abstract

The invention provides a method for preparing indigo naturalis, comprising the following steps of: a, placing leaves or stem leaves of strobilanthus (acanthaceae), indigo (polygonaceae) or woad (crucifer) into water with the pH value of 3.5-7 at the temperature of 25-60 DEG C, the mass ratio of water to the fresh leaves is 8-15, soaking for 24-48 hours, and filtering and removing leaf slag to obtain filtrate; b, introducing air into the filtrate for 0-30min, adding alkaline to regulate the pH value to be 9-10.5, introducing the air for 30-240min, adding lime milk, stirring to be uniform and standing, filtering to obtain precipitate, drying to obtain crude indigo, and refining the crude indigo to finally obtain the indigo naturalis. In the crude indigo prepared by adopting the method provided by the invention, contents of indigo blue and indirubin are higher, and product quality is good.

Description

Technical field [0001] The invention relates to a preparation method of Indigo Naturalis, which belongs to the field of medicine. Background technique [0002] Indigo Naturalis is a dry powder or agglomerate obtained by processing the leaves or stems of the Acanthaceae plant Ma Lan, Polygonaceae plant Indigo blue or Cruciferae plant Isatis indigotica. The processing process is the transformation of the precursor substances in the stems and leaves of the horse blue stems and leaves into indigo and indirubin, which mainly includes soaking, making crude indigo, and water flying refining. The traditional processing method is: harvest the stems and leaves in autumn, put them in the soaking tank, introduce the river water, submerge them completely, soak and ferment for several days, when the pool liquid is dirty and green, remove the residue, put in lime, and stir thoroughly. Let it stand for 1 to 2 days, discard the supernatant, collect the sediment at the bottom and move it to a sma...

Claims

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Application Information

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IPC IPC(8): A61K36/19A61K36/315A61K36/704
Inventor 杨明傅超美许润春刘泽玉苏柘僮
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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