Preparation method and application of eclipta extract

A Eclipta chinensis extract and Eclipta chinensis technology are applied in the directions of medical preparations, drug combinations, and pharmaceutical formulations containing active ingredients, can solve problems such as the Eclipta chinensis extract has not been disclosed, and achieve environmental pollution-free, reduced The effect of purification costs

Inactive Publication Date: 2011-11-23
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the prior art scheme, the Eclipta chinensis extract with stable co

Method used

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  • Preparation method and application of eclipta extract
  • Preparation method and application of eclipta extract
  • Preparation method and application of eclipta extract

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preparation example Construction

[0016] The steps of the preparation method of Eclipta chinensis extract are as follows:

[0017] 1) Select the Eclipta chinensis plant, soak it in ethanol / water with a volume percentage concentration of 5-95% for 0.5 hours, extract 1 hour with ethanol / water that is 20 times the weight of the medicinal material Eclipta chinensis, and ethanol / water is the ink Extract 16 times the weight of the Eclipta medicinal material for 0.5h, combine the extract, concentrate the extract to 0.25-1.0g crude drug / mL, let it stand for 6h, and filter to obtain the Eclipta extract;

[0018] 2) Eclipta extract is adsorbed by pretreated macroporous resin, the weight ratio of Eclipta extract to macroporous resin is 1:1~1:5, and the sample flow rate is 1~3 BV / h. The ratio of diameter to height is 1:4~1:7. After adsorption, wash with water for 3~6 BV, the elution flow rate is 2~4BV / h, and discard the water eluent; then use 20~90% ethanol with volume percentage concentration / water elution of 3-6 BV, t...

Embodiment 1

[0027] Embodiment 1: the preparation of Eclipta chinensis extract

[0028] Eclipta medicinal material 10kg, soaked in ethanol / water with a volume percentage concentration of 5% for 0.5 hours, the ethanol / water is 20 times the weight of the medicinal material Eclipta 1h and the ethanol / water is the extraction of 16 times the weight of the medicinal material Eclipta 0.5 h, combine the extracts, concentrate the extracts to 0.25 g crude drug / mL, let stand for 6h, and filter to obtain the extract of Eclipta chinensis; The weight ratio of the resin is 1:1, the sample loading flow rate is 1 BV / h, and the diameter-to-height ratio of the resin column is 1:4. The volume percentage concentration was 20% ethanol / water to elute 3 BV, the flow rate was 2BV / h, and the volume percentage concentration was 20% ethanol / water eluate, concentrated and dried to obtain the Eclipta officinalis extract. The mass percent contents of Eclipta saponin B, A, and C in the Eclipta japonica extract were me...

Embodiment 2

[0029] Embodiment 2: the preparation of Eclipta chinensis extract

[0030]Eclipta medicinal material 10kg, soaked in ethanol / water with a volume percentage concentration of 95% for 0.5 hours, the ethanol / water is 20 times the weight of the medicinal material Eclipta 1h and the ethanol / water is the extraction of 16 times the weight of the medicinal material Eclipta 0.5 h, combined the extracts, concentrated the extracts into 1.0 g crude drug / mL, left standstill for 6h, and filtered to obtain the Eclipta extract; the Eclipta extract was passed through the LX60 macroporous resin, and the Eclipta extract and the macroporous resin were separated The weight ratio is 1:5, the sample loading flow rate is 3BV / h, and the diameter-to-height ratio of the resin column is 1:7. After adsorption, wash 6BV with water first, and the elution flow rate is 4BV / h. The concentration is 90% ethanol / water to elute 6 BV, the flow rate is 4 BV / h, the eluate with a volume percentage concentration of 90...

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Abstract

The invention discloses a preparation method and application of eclipta extract. The preparation method comprises the following steps of: 1) selecting eclipta, soaking for 0.5 hour with ethanol water solution with the volume percent concentration of 5-95%, extracting for 1 hour when the weight of the ethanol water solution is 20 times of the weight of eclipta and extracting for 0.5 hour when the weight of the ethanol water solution is 16 times of the weight of the eclipta, mixing extracts together, concentrating the extract into 0.25-1.0g crude drug/ml, standing for 6 hours, and filtering to obtain the eclipta extract; and 2) adsorbing the eclipta extract by adopting pretreated macroporous resin, wherein the weight ratio of the eclipta extract to the macroporous resin is 1:1-1:5, feeding flow rate is 1-3BV/h and the diameter height ratio of the resin is 1:4- 1:7, washing for 3-6BV with water after adsorbing, wherein eluting flow rate is 2-4BV/h, throwing away eluent; eluting for 3-6BV with the ethanol water solution with the volume percent concentration of 20-90%, wherein the flow rate is 2-4BV/h, collecting the ethanol/water eluent, concentrating, and drying to obtain the eclipta extract. By adopting the preparation method disclosed by the invention, purification cost is reduced, industrial production is easy to realize, and no environment pollution is caused.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicines, and in particular relates to a preparation method and application of an extract of Eclipta chinensis. Background technique [0002] Eclipta chinensis is a plant of the genus Channa in the family Asteraceae ( Eclipta prostrata ) dry aboveground part, is a traditional Chinese medicine, has the effect of nourishing the liver and kidney, cooling blood and stopping bleeding, and is mainly used to treat loose teeth, premature graying of beard and hair, dizziness and tinnitus, soreness of waist and knees, blood heat due to yin deficiency, hematemesis, epistaxis, hematuria, and bloody dysentery , Metrorrhagia, bleeding from trauma. Eclipta is often used as a compound medicine. For example, the traditional prescription Erzhi Pill comes from Ming Wang Sancai's "Yibian". Dizziness and tinnitus caused by kidney yin deficiency, dry mouth and nose, restless heat and insomnia, hematemesis, metrorr...

Claims

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Application Information

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IPC IPC(8): A61K36/28A61P1/02A61P1/14A61P1/16A61P3/06A61P7/04A61P29/00A61P37/04A61P39/00
Inventor 田景奎马鲁豫戚中杰李锡敏郑雯傅之容
Owner ZHEJIANG UNIV
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