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Method for rapidly preparing denudatine from long-handle aconitum szechenyianum gay plant

The technology of photodelphin and iron hammer is applied in the field of rapid preparation of photodelphin, which can solve the problems such as no extraction of delphine, and achieve the effect of simple preparation process.

Inactive Publication Date: 2011-11-23
CHENGDU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no report on the extraction and separation of photodelphinidin from the root of the long-handled iron rod hammer

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Get 1000g of long-handled iron bar hammer root tubers, pulverize it, soak 24 hours at room temperature with 5000g of hydrochloric acid aqueous solution of pH 2, then use the hydrochloric acid aqueous solution of 8000ml of pH 2 to percolate and extract 3 times, combine the extracts, filter Precipitate, and then use ammonia water to alkalize the percolation extract to pH 10;

[0017] (2) extracting the percolation extract after above-mentioned alkalization with ethyl acetate for 3 times, washing the extract with water to neutrality, drying with anhydrous sodium sulfate, concentrating the extract under reduced pressure to obtain the total alkaloid extract;

[0018] (3) Put the total alkaloid extract prepared in step (2) into a container with acetone for dissolution, turn out the acetone solution after it is fully dissolved, then scrape off the white insoluble matter on the container wall, and wash it with acetone Wash the white insolubles, and finally dry the white ins...

Embodiment 2

[0020] (1) Get 1000g of long-handled iron bar hammer root tubers, pulverize it, soak 30 hours at room temperature with 5000g of acetic acid aqueous solution of pH 5, then use the acetic acid aqueous solution of 8000ml of pH 5 to percolate and extract 3 times, combine the extracts, filter Precipitate, then use sodium hydroxide to alkalinize the percolation extract to pH 10;

[0021] (2) extracting the percolation extract after above-mentioned alkalization with ether twice, washing the extract with water until neutral, drying with anhydrous sodium sulfate, concentrating the extract under reduced pressure to obtain the total alkaloid extract;

[0022] (3) Put the total alkaloid extract prepared in step (2) into a container equipped with ethanol for dissolving, turn out the ethanol solution after it is fully dissolved, then scrape off the white insoluble matter on the container wall, and use ethanol Wash the white insolubles, and finally dry the white insolubles after washing to o...

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Abstract

The invention discloses a method for rapidly preparing denudatine from a long-handle aconitum szechenyianum gay plant. The method comprises the following steps: by taking a ranunculaceae aconitum plant long-handle aconitum szechenyianum gay as a raw material, drying and smashing the raw material; soaking the smashed raw material for 1-72 hours with an acidic aqueous solution with the pH value of 1-6 at room temperature; then carrying out percolation extraction with an acidic aqueous solution with the pH value of 1-6; after alkalizing percolation extract until the pH value of the extract is 8-12, extracting with an organic solvent, rinsing extract liquor, and concentrating the extract liquor so as to obtain total alkaloid; and finally, separating out the denudatine by utilizing different dissolvability of different alkaloids in the total alkaloid in the organic solvent. The purity of the denudatine prepared by the method can reach above 95%, and the yield of the denudatine prepared by the method is up to 0.3%.

Description

technical field [0001] The invention relates to a method for extracting and isolating photodelphinidine from plants of the genus Aconitum of Ranunculaceae, in particular to a method for rapidly preparing photodelphinidinine from a long-handled iron bar hammer. Background technique [0002] Diterpene alkaloids are an important class of natural compounds. There are more than 900 known natural diterpene alkaloids, mainly found in Aconitium, Delphinium and Spirea. In recent decades, due to the wide application of spectrum technology in structural analysis, the research on diterpene alkaloids has been developed rapidly. At the same time, the medicinal research of diterpene alkaloids has also achieved certain results. Lappaconitine A, 3-acetylaconitine and crassicauline A have been used clinically as new non-addictive analgesic and anti-inflammatory drugs. [0003] Plants of the genus Aconitum are distributed in all provinces in Southwest my country, and many of them are used as ...

Claims

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Application Information

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IPC IPC(8): C07D221/22
Inventor 陈封政苟小军刘嵬李书华宋芹颜军何刚
Owner CHENGDU UNIV
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