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A method for preparing lysophosphatidylcholine by enzymatic alcoholysis

A technology for phosphatidylcholine and catalyzing alcoholysis, which is applied in the field of lipase-catalyzed alcoholysis of phosphatidylcholine and alcohol to prepare lysophosphatidylcholine, and can solve the problem that the solubility of phosphatidylcholine is not very good and the pH value of the system is poor. Influence and other problems, to achieve the effect of stable activity, good repeatability and good solubility

Inactive Publication Date: 2011-12-14
HENAN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of preparing lysophosphatidylcholine by solvent hydrolysis is that the solubility of phosphatidylcholine in n-hexane is not very good, and a large amount of fatty acid will be produced in the hydrolysis reaction, which will affect the pH value of the system

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Put 30g of phosphatidylcholine and 100mL of ethanol / water solution (ethanol / water=99.8:0.2, v / v) in a 250mL round bottom flask; connect the reflux device, heat (30°C) and stir to make the phosphatidylcholine Dissolve completely in ethanol / water solution; weigh 4.5g Lipozyme TLIM and add it to the reactant, react at 30°C for 20h, stop the reaction, remove lipase by centrifugation or filtration, and remove the reactant- ---Ethanol, dissolved to obtain the primary product of lysophosphatidylcholine.

Embodiment 2

[0026] Put 15g of phosphatidylcholine and 100mL of ethanol / water solution (ethanol / water=92:8, v / v) in a 250mL round bottom flask; connect the reflux device, heat (30°C) and stir to make the phosphatidylcholine Completely dissolve in ethanol / water solution; weigh 2.25g Lipozyme TLIM and add it to the reactant, react at 30°C for 16h, stop the reaction, remove lipase by centrifugation or filtration, and remove the reactant from the reaction mixture by vacuum ----Ethanol, dissolved to get the primary product of lysophosphatidylcholine.

Embodiment 3

[0028] Put 20g of phosphatidylcholine and 10mL of ethanol / water solution (ethanol / water=95:5, v / v) in a 150mL round bottom flask; connect the reflux device, heat (30°C) and stir to make the phosphatidylcholine completely Dissolve in ethanol / water solution; weigh 3g Lipozyme RMIM and add it to the reactant, react at 30°C for 40h, then stop the reaction, remove lipase by centrifugation or filtration, and remove the reactant from the obtained reaction mixture by vacuum --- -Ethanol, dissolved to obtain the primary product of lysophosphatidylcholine.

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Abstract

The invention discloses a method for preparing lysophosphatidyl choline by enzymatic alcoholysis, which comprises: adding phosphatidylcholine into low-carbon alcohol solution, uniformly stirring and mixing at a certain temperature, adding lipase, and allowing the lipase to catalyze the alcoholysis of phosphatidylcholine with constant-temperature stirring to obtain the lysophosphatidyl choline. The method has the advantages that: (1) the solubility of phosphatidylcholine, lysophosphatidyl choline serving as the product, and aliphatic ester, glycerophosphorylcholine and very small amount of fatty acid, which serve as byproducts, in the reaction system is improved, and the conversion rate of the lysophosphatidyl choline is high; (2) the aliphatic ester, a small amount of glycerophosphate and a very small amount of fatty acid are generated in an alcoholysis reaction process, but the pH value of the system is not changed, and the influence of the solvent on the activity of the lipase is relieved and the catalytic reaction activity of the lipase is high; (3) the properties of the fatty acid, the aliphatic ester and glycerophosphorylcholine, which are products of side reactions, are very different from those of the lysophosphatidyl choline, so the lysophosphatidyl choline product can be separated and purified very easily; and (4) the alcohol, which is the product of the reaction, can be removed easily.

Description

[0001] technical field [0002] The invention relates to a method for preparing lysophosphatidylcholine by enzymatically catalyzing the alcoholysis of phosphatidylcholine, specifically using lipase to catalyze alcoholysis of phosphatidylcholine and alcohol to prepare lysophosphatidylcholine in a solvent-free system Alkaline method. [0003] Background technique [0004] Lysophosphatidylcholine is the product obtained by removing a fatty acyl group from phosphatidylcholine. Phosphatidylcholine (also known as lecithin) is widely found in animals and plants, and it is more abundant in the brain, adrenal gland and cells of animals. Compared with phosphatidylcholine, lysophosphatidylcholine has a larger HLB value (hydrophilic-lipophilic balance value), which not only retains lipophilicity, but also increases its hydrophilicity due to the reduction of non-polar groups. Wetting ability, emulsifying ability and emulsifying ability are all better than phosphatidylcholine. Lysopho...

Claims

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Application Information

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IPC IPC(8): C12P13/00
Inventor 杨国龙胡静波毕艳兰孙尚德刘伟王渝鹭张洁
Owner HENAN UNIVERSITY OF TECHNOLOGY
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