Preparation method for jelly fish neurotensin

A technology of antihypertensive peptides and jellyfish, which is applied in the field of preparation of polypeptide mixtures, can solve the problems of poor activity preservation, low purity, and low yield of antihypertensive peptides, and achieve the effects of reduced production costs, high extraction rates, and simple operations

Active Publication Date: 2013-12-04
吴川市天然食品加工有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] There have been reports at home and abroad to extract antihypertensive peptides from jellyfish or other marine animal and plant resources, but the obtained products are basically bold proteins or centrifuged supernatants, and the partial yield of antihypertensive peptides is low, low in purity, and poorly preserved in activity. There are many deficiencies as a raw material for antihypertensive products

Method used

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  • Preparation method for jelly fish neurotensin

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Removal of various metal ions in jellyfish: First, use an ultrapure water machine to extract deionized water, which requires an impedance value of not less than 10. Mix the two according to the mass ratio of salted jellyfish to ultrapure water of 1:2, and stir for 30 Minutes; then extract deionized water from the ultrapure water machine, requiring an impedance value of not less than 15, mix the two according to the mass ratio of salted jellyfish to ultrapure water of 1:2, and stir for 30 minutes; The machine extracts deionized water, and the impedance value is required to be not less than 18. According to the mass ratio of salted jellyfish to ultrapure water of 1:1, mix the two and stir for 15 minutes.

[0027] Drain the jellyfish after removing the metal ions, put it into a tissue meat grinder and grind it; put the ground jellyfish into a homogenizer, and homogenize it at 5000r / min for 10 minutes.

[0028] Dilute the homogenized jellyfish slurry 100 times with ultrapur...

Embodiment 2

[0032] First, pre-treat the salted jellyfish to remove various metal ions in the jellyfish, and extract deionized water from the ultrapure water machine. The impedance value is required to be not less than 10. According to the quality of salted jellyfish to ultrapure water is 1:2 Mix the two and stir for 30 minutes; then extract deionized water from the ultrapure water machine, requiring an impedance value of not less than 15, mix the two according to the mass ratio of salted jellyfish to ultrapure water of 1:2, and stir 30 minutes; finally extract deionized water from the ultrapure water machine, the impedance value is required to be no less than 18, mix the two according to the mass ratio of salted jellyfish to ultrapure water of 1:1, and stir for 15 minutes.

[0033] Take out the jellyfish and drain the water, put it into the tissue meat grinder and grind it; put the ground jellyfish into the homogenizer, and homogenize it at 5000r / min for 10 minutes; the jellyfish slurry af...

Embodiment 3

[0038] First, pre-treat the salted jellyfish to remove various metal ions in the jellyfish, and extract deionized water from the ultrapure water machine. The impedance value is required to be not less than 10. According to the quality of salted jellyfish to ultrapure water is 1:2 Mix the two and stir for 30 minutes; then extract deionized water from the ultrapure water machine, requiring an impedance value of not less than 15, mix the two according to the mass ratio of salted jellyfish to ultrapure water of 1:2, and stir 30 minutes; finally extract deionized water from the ultrapure water machine, the impedance value is required to be no less than 18, mix the two according to the mass ratio of salted jellyfish to ultrapure water of 1:1, and stir for 15 minutes.

[0039] Take out the jellyfish and drain the water, put it into a tissue meat grinder and grind it; put the ground jellyfish into a homogenizer, and homogenize it at 5000r / min for 10 minutes.

[0040]Dilute the homogen...

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Abstract

A preparation method of jellyfish antihypertensive peptide, comprising: removing metal ions from jellyfish, followed by homogenization, enzymatic hydrolysis, enzyme inactivation, concentration, ultrafiltration, and freeze-drying to obtain purified jellyfish antihypertensive peptide powder. This method combines modern enzyme engineering technology to provide a new and efficient production method of jellyfish antihypertensive peptide, which improves the utilization efficiency of jellyfish resources, and has the characteristics of simple operation, high extraction rate and good product activity preservation. Peptide production costs are reduced. It makes full use of the rich protein resources of jellyfish, and basically removes all kinds of metal ions in jellyfish through metal ion removal treatment, making subsequent enzymatic hydrolysis and application safer. The results of the antihypertensive activity test of the jellyfish antihypertensive peptide with the hypertensive SHR rat model show that the group of the present invention has a significant difference compared with the blank control group, and the jellyfish antihypertensive peptide prepared by this method has a similar activity to that of the positive control captopril. hypotensive activity.

Description

technical field [0001] The invention relates to a preparation method of a polypeptide mixture, in particular to a preparation method of a jellyfish antihypertensive peptide. Background technique [0002] Hypertension is a disease that seriously harms human health, and it is also the main factor that causes cardiovascular diseases. Its harm is to cause damage to important organs such as the brain, heart, and kidneys. Stroke and coronary heart disease are the most serious complications of hypertension. Data show that the average incidence rate of the world is 10% to 20%, and the number of deaths due to hypertension reaches 12 million every year. At present, there are more than 120 million hypertensive patients in my country. Therefore, the treatment and prevention of hypertension is a very important topic in today's society. [0003] At present, medical research on hypertension mainly focuses on ACE inhibitors (ACEI). Currently, chemically synthesized ACEIs such as Captopr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/34
Inventor 林靖善胡光耀
Owner 吴川市天然食品加工有限公司
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