Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of drug carried microsphere capable of being positioning injected into tumor cavity

A technology of drug-loaded microspheres and polylactic acid, which is applied in anti-tumor drugs, pharmaceutical formulas, medical preparations of non-active ingredients, etc.

Inactive Publication Date: 2012-01-04
TIANJIN UNIV
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the shortcomings of the above-mentioned existing paclitaxel injections, and propose a preparation method for drug-loaded microspheres that can be positioned and injected into tumor cavities with simple process and excellent performance.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of drug carried microsphere capable of being positioning injected into tumor cavity
  • Preparation method of drug carried microsphere capable of being positioning injected into tumor cavity
  • Preparation method of drug carried microsphere capable of being positioning injected into tumor cavity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Preparation of drug-loaded microspheres that can be injected into tumor cavity

[0027] 1) Accurately weigh 8.0 mg of polylactic acid and 2.0 mg of paclitaxel and place in a vial, dissolve with 1.0 ml of acetone, stir well and set aside. Add 1mg of BSA to 10ml of water under constant stirring;

[0028] 2) Take 10.0ml of deionized water and put it in a 25ml beaker, put it into a large magnetic stirrer, start stirring and adjust the speed, so that the magnet rotates smoothly without hitting the wall, and the liquid surface is in a very good spindle shape;

[0029] 3) Use a 1ml disposable syringe to suck out the acetone solution and inject it into the beaker at one time. The injection should be slow and even, and the injection needle should be below the liquid level;

[0030] 4) After stirring for 3 hours, until the acetone is completely volatilized, an opalescent suspension is obtained, and freeze-dried to obtain white PLA drug-loaded nanoparticle powder.

[0031] 5...

Embodiment 2

[0033] 1) Accurately weigh 9.0mg of polylactic acid and 1.0mg of paclitaxel in a vial, dissolve with 1.0ml of acetone, stir well and set aside. Add 1mg of BSA to 10ml of water under constant stirring;

[0034] 2) Take 10.0ml of deionized water and put it in a 25ml beaker, put it into a large magnetic stirrer, start stirring and adjust the speed, so that the magnet rotates smoothly without hitting the wall, and the liquid surface is in a very good spindle shape;

[0035] 3) Use a 1ml disposable syringe to suck out the acetone solution and inject it into the beaker at one time. The injection should be slow and even, and the injection needle should be below the liquid level;

[0036] 4) After stirring for 3 hours, until the acetone is completely volatilized, an opalescent suspension is obtained, and freeze-dried to obtain white PLA drug-loaded nanoparticle powder.

[0037] 5) Example 2 obtains the nano drug-loaded microspheres, observes the scanning electron microscope picture o...

Embodiment 3

[0039] 1) Accurately weigh 8.0 mg of polylactic acid and 2.0 mg of paclitaxel and place in a vial, dissolve with 1.0 ml of acetone, stir well and set aside. Add 2mg of BSA to 10ml of water under constant stirring;

[0040] 2) Take 10.0ml of deionized water and put it in a 25ml beaker, put it into a large magnetic stirrer, start stirring and adjust the speed, so that the magnet rotates smoothly without hitting the wall, and the liquid surface is in a very good spindle shape;

[0041] 3) Use a 1ml disposable syringe to suck out the acetone solution and inject it into the beaker at one time. The injection should be slow and even, and the injection needle should be below the liquid level;

[0042] 4) After stirring for 3 hours, until the acetone is completely volatilized, an opalescent suspension is obtained, and freeze-dried to obtain white PLA drug-loaded nanoparticle powder.

[0043] 5) The nano drug-loaded microspheres obtained in Example 3, after freeze-drying, observe the s...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention relates to a preparation method of a drug carried microsphere capable of being positioning injected into a tumor cavity. When in a reaction process, the nano drug carried microsphere is prepared through a nano precipitation method by adopting polylactic acid as a carrier of an anti-cancer drug taxol and adopting bovine serum albumin as surfactant. Under an opsonic action of a reticuloendothelial system, after the drug is packed by polymer, the pharmacokinetic behaviors of the drug in vivo are always changed. The nano microsphere can be prepared into a slow-releasing preparation so that the half life of the drug in vivo is changed and the drug action time is prolonged; therefore, on condition of ensuring the drug effect, the dosage of the drug is reduced, and adverse effects are reduced or eliminated. Synchronously, the BSA (Bovine Serum Albumin) is served as external water phase to prepare polylactide particles, the bovine serum albumin can be absorbed on the surface of the microspheres so as to be easy to be identified by the reticuloendothelial system and absorbed by liver, therefore, the drug carried microspheres are gathered at the liver, the drug is slowly released at the liver, and the action time of the drug is prolonged.

Description

technical field [0001] The invention relates to a preparation method of drug-loaded microspheres capable of localized injection in tumor cavities; in particular, a preparation method of drug-loaded microspheres capable of localized injection of tumor cavities. Background technique [0002] Drug-loaded nanoparticles are particles formed by encapsulating drugs, biologically active substances, etc. inside nanoparticles, or adsorbing or connecting to the surface of particles, or mixing and dissolving in material matrices. between). Due to the characteristics of small size effect, quantum size effect, surface effect and macroscopic quantum tunneling effect, polymer drug-loaded nanoparticles are beneficial to be absorbed by diseased tissues and cells, so that they may become excellent drug carriers. Due to the conditioning effect of the reticuloendothelial system, the pharmacokinetic behavior of the drug in the body is often changed after the drug is entrapped in the polymer. Na...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K9/19A61K31/337A61K47/42A61K47/34A61P35/00
Inventor 原续波秦姚姚
Owner TIANJIN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com