Method for extracting and purifying lycoris radiata lectin from lycoris radiata bulbs and lycoris radiata lectin produced by method

A technology of lycoris bulbs and lectins, which is applied in the field of lycoris lectins, can solve the problems of low product quality and achieve the effect of leaping yield, high yield and improved extraction rate

Active Publication Date: 2012-01-04
贵州芊芊园艺新技术发展公司
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Problems solved by technology

[0005] The master's degree thesis of Sichuan University School of Life Sciences "Purification, Properties and Conformation of Lycoris Lectin" has a brief introduction to the preparation and purification of Lycoris Lectin. Since this paper is mainly for research purposes, although some process steps Same as the present invention, but its process steps and process conditions are very simple, the content of lycoris agglutinin is only about 60%, and the product quality is not high

Method used

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  • Method for extracting and purifying lycoris radiata lectin from lycoris radiata bulbs and lycoris radiata lectin produced by method
  • Method for extracting and purifying lycoris radiata lectin from lycoris radiata bulbs and lycoris radiata lectin produced by method
  • Method for extracting and purifying lycoris radiata lectin from lycoris radiata bulbs and lycoris radiata lectin produced by method

Examples

Experimental program
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Effect test

example 1

[0052] (1). Raw material preparation: 11.2Kg of freshly excavated Lycoris bulbs, 1.1Kg of fibrous roots, silt, and sundries are removed, and 10.0Kg of Lycoris bulbs are left. The content of Lycoris lectin is 0.15% after testing.

[0053] (2). Beating: the fresh raw material pieces are pulverized and beaten by a pulverizer to obtain 10Kg of pulpy raw materials;

[0054] (3). Centrifugation for the first time: Separating the pulpy raw material with a centrifuge at 3500 rpm to obtain centrifuged liquid 1 and centrifuged solid phase 1, which are respectively 6.0Kg for liquid and 4.0Kg for solid phase;

[0055] (4). Enzymatic extraction;

[0056] (5). The second centrifugation: Add the centrifugal solid phase 1 to 8Kg of physiological saline, which contains 0.05ml / l of plant compound enzyme, the concentration of plant compound enzyme is 105000u / ml, the stirring speed is 50rpm, and the temperature is normal temperature. Extracted for 2 hours, centrifuged at 3500rpm in a tubular cen...

example 2

[0076] Take 5Kg of lycoris cadmium powder, the content of Lycoris lectin is 0.4% after testing, use 45Kg of normal saline 9 times the weight of dry powder to extract three times, the ratio of normal saline for three extractions is 5:3:1, normal saline Contain plant complex enzyme 0.05ml / l in it, the concentration of plant compound enzyme is 105000u / ml (leaching time is 4-5 hours, extraction speed is 50rpm), each time through tubular centrifuge 3500rpm centrifugal filtration, get clear liquid and slag, combined clear liquid, add ammonium sulfate solution, take the saturation degree of ammonium sulfate at 30%-70% and pass through tubular centrifuge 3500rpm centrifugal filtration, the obtained lycoris agglutinin crude product (slag) is put into the dialysis bag, Dialyze with pure water to desalinate until no sulfate ions can be detected with barium chloride solution as the end point of dialysis to obtain a sample solution, dissolve the crude Lycoris agglutinin in 0.02mol / L NaAC-HA...

example 3

[0080] (1). Raw material preparation: 56Kg of freshly excavated Lycoris bulbs, remove 6Kg of fibrous roots, silt, and sundries, leaving 50.0Kg of Lycoris bulbs, the content of Lycoris lectin is 0.14% after testing,

[0081] (2). Beating: the fresh raw material pieces are pulverized and beaten by a pulverizer to obtain 50Kg of pulpy raw materials;

[0082] (3). Centrifugation for the first time: Separating the pulpy raw material with a centrifuge at 3500 rpm to obtain centrifuged liquid 1 and centrifuged solid phase 1, which are respectively 30.0Kg for liquid and 20.0Kg for solid phase;

[0083] (4). Enzymatic extraction;

[0084] (5). The second centrifugation: add the centrifugal solid phase 1 to 40Kg of physiological saline, which contains 0.05ml / l of plant compound enzyme, the concentration of plant compound enzyme is 105000u / ml, the stirring speed is 50rpm, and the temperature is normal temperature. Extracted for 2 hours, centrifuged at 3500rpm in a tubular centrifuge to ...

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Abstract

The invention discloses a method for extracting and purifying lycoris radiata lectin from lycoris radiata bulbs and the lycoris radiata lectin produced by the method. The lycoris radiata bulbs are taken as raw materials and are extracted and purified by a specific process to form pure lycoris radiata lectin with a single protein band, and the pure lycoris radiata lectin has the apparent molecular weight of 48KD, is protein consisting of four subunits with the same molecular weight, is amorphous powder and has the lycoris radiata lectin content of 90 percent. The method comprises the following process steps of: (1) preparing raw materials; (2) pulping; (3) performing centrifugal separation for the first time; (4) leaching; (5) performing centrifugal separation for the second time; (6) mixing centrifugal separation solutions 1 and 2; (7) performing fractional precipitation; (8) performing centrifugal separation for the third time; (9) performing dialysis demineralizing for the first time; (10) performing electrochromatophoresis separation; (11) performing molecular sieve chromatography; (12) eluting; (13) performing dialysis demineralizing for the second time; (14) performing vacuum concentration; and (15) performing vacuum drying. The invention provides a method capable of extracting the lycoris radiata lectin, lycoris radiata polypeptides and lycoris radiata gelatin and also capable of extracting and separating total alkaloids of lycoris radiata; the process is simple; and the energy is saved and the consumption is reduced.

Description

technical field [0001] The present invention relates to a method for extracting and purifying effective ingredients from the bulbs of Lycoris, in particular to a method for extracting and purifying Lycoris agglutinin from bulbs of Lycoris and the Lycoris agglutinin produced by the method. Background technique [0002] Lycoris radi a toAgglutinin (sub-corl s ra dl a to) belongs to the Amaryllidaceae plant, aliased as lycori s radi a toAgglutinin, a perennial herb with oval bulbs, rich in lectin (Lycori s radi a toAgglutinin for short) LRA) Lycoris is a perennial herbaceous bulb flower with many varieties, including red flowers, yellow flowers, white flowers and other types. In plant taxonomy, it belongs to the genus Lycoris (Lycoris.herb) of the monocotyledonous plant Amaryllidaceae (Amaryllidaceae). There are about 20 species of Lycoris in the world, and there are 15 species in my country, which occupy an absolute advantage and are distributed in 12 provinces and cities. , A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/42C07K1/36C07K1/34C07K1/30C07K1/26C07K1/16
Inventor 黄玉群石贵阳陈光强刘涛
Owner 贵州芊芊园艺新技术发展公司
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