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Method for preparing antioxidant from microbes

An antioxidant and microbial technology, applied in the field of antioxidant preparation, can solve the problems of many impurities and low antioxidant activity, and achieve the effect of high antioxidant activity

Inactive Publication Date: 2013-06-05
HEILONGJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to solve the problem that the bacteria-produced antioxidants obtained by the existing extraction method have more impurities and low antioxidant activity, and provide the antioxidants derived from microorganisms Preparation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0009] Specific Embodiment 1: The preparation method of the antioxidant derived from microorganisms in this embodiment is carried out according to the following steps: 1. Inoculate Penicillium 1-2 Cyclobacterium JTLR-1 on PDA solid medium, cultivate it at 28°C for 2 days, and then transfer In a 500mL Erlenmeyer flask filled with 200mL of Cha's medium, shake the flask for 7 days at 28°C and 180r / min to collect the liquid fermentation culture; After repeated freezing and thawing of liquid nitrogen and crushing, add distilled water to form a fluid passing through a 40-mesh standard sieve, then extract with n-hexane until the n-hexane extract is colorless, then take the water layer and add extraction solvent for extraction, extract three times, and combine the three extracts , then add anhydrous Na 2 SO 4 Remove water, remove solids by filtration to obtain an extract, and then remove the solvent under reduced pressure at 25-50°C to obtain anhydrous extract; 3. Dissolve 1g of anhy...

specific Embodiment approach 2

[0010] Specific embodiment two: the difference between this embodiment and specific embodiment one is that the PDA solid medium in step one is made up of 100ml mass concentration of 20% potato juice, 2g sucrose and 2g agar. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0011] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is that in the step one, the NaNO of 2g is used for every L of Cha's culture medium 3 , 1g of K 2 HPO 4 , 0.5g of KCl, 0.5g of MgSO 4 4H 2 O, 0.01g of FeSO 4 , 30g of sucrose and the remainder of distilled water, the pH value is natural. Other steps and parameters are the same as those in Embodiment 1 or Embodiment 2.

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Abstract

The invention relates to a method for preparing an antioxidant from microbes, and relates to a method for preparing an antioxidant. By the method, the problems of more impurities and low antioxidation activity in the bacterium-produced antioxidant obtained in the conventional extraction method are solved. The method comprises the following steps of: preparing a culture of penicillium JTLR-1; freezing and thawing the culture repeatedly, and grinding to prepare an anhydrous extract; preparing ethyl acetate solution of the anhydrous extract, and loading on a silica gel filling, and removing the ethyl acetate solution; mixing the ethyl acetate solution and absolute ethanol, eluting silica gel, and removing eluent; and continuing to elute the silica gel, collecting the eluent, and removing a solvent under reduced pressure to obtain the antioxidant. By the method, more impurities can be removed, and compared with the conventional extraction method, the method has the advantages that the obtained antioxidant is concentrated by at least over 6 times and is high in the antioxidation activity.

Description

technical field [0001] The invention relates to a preparation method of an antioxidant. Background technique [0002] Free radicals are intermediate metabolites of various biochemical reactions in life activities. Excessive generation of free radicals or slow clearance will cause various damages to the body at the molecular level, cellular level, and tissue and organ level, accelerate the aging process of the body and induce various diseases. In the process of aging, the balance between the oxidation and antioxidant systems in the body changes, which is manifested by enhanced oxidation and weakened antioxidant capacity. With age, the activity of antioxidant enzymes such as SOD decreases, and the body is free The ability of free radicals is affected, and the concentration of oxygen free radicals increases, causing membrane lipid peroxidation, protein denaturation, enzyme activity reduction, and DNA damage. The changes in the structure and function of some biological macromol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P1/02C12R1/80
Inventor 金涛葛菁萍
Owner HEILONGJIANG UNIV