Liquid-based cell preservation liquid composite and preparation method thereof

Abstract

A liquid-based cell preservation liquid composite is characterized by comprising the following components in percentage by weight: cell fixing agent: 2-15% of formaldehyde, preservative: 0.1-5% of sodium diacetate, anticoagulant: 2-10% of sodium citrate, buffering agent: 0.5-2% of sodium chloride, chelating agent: 0.002-0.5% of ethylene diamine tetraacetic acid (EDTA), hemolytic agent: 0.5-10% ofglacial acetic acid, and water: 5-80% of pure water, wherein the formaldehyde is the analytical reagent, and accounts for 37-40%; the sodium diacetate is the food additives; the sodium citrate and the sodium chloride are analytical reagents, and NaCl is more than 99.5%; and the EDTA and the glacial acetic acid are analytical reagents, and H2COOH is more than 99.5%. The invention has the advantages that the liquid-based cell preservation liquid composite can keep the cell form to the maximum extent, can improve the diagnosis accuracy and can prevent the cell form of a specimen from significantdifference at room temperature for at least three months.

Description

technical field [0001] The invention relates to a reagent for liquid-based thin-layer cell detection, in particular to a liquid-based cell preservation solution composition and a preparation method thereof. Background technique [0002] In the field of liquid-based thin-layer cell detection technology, the fully automatic membrane-type liquid-based ultra-thin cell preparation system Xinpai TCT2000 processor and its supporting special reagent Cell Preservation Solution (PreserveCyt Solution) produced by American CCID Company are known as liquid The gold standard for cytology testing. Chinese Utility Model Patent No. 200620141836 also disclosed a membrane-type liquid-based cell sheeting machine on December 30, 2006, including a solution bottle for storing cell liquid base, a cell collection device and a controller for collecting cells in the solution bottle. The cell collection device includes a filter and a pressure supply device for providing pressure to the filter, the fil...

AI Technical Summary

Problems solved by technology

Since this liquid-based cell preservation solution is specially used in conjunction with the membrane-type liquid-based cell production machine, it is impossible to achieve the natural precipitation method to separate the mucus, blood and inflammatory cells in the specimen to achieve the accuracy of the smear. Higher quality and higher efficiency; and this liquid-based cell preservation solution can only preserve the prepared specimens without change for four weeks, which is difficult for some intractable diseases that require repeated smear specimens, and cannot meet the requirements of case studies. Need, not conducive to patient treatment

[0004] Therefore, there are above-mentioned inconveniences and problems in the known liquid-based cell preservation solution.

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