Liquid-based cell preservation liquid composite and preparation method thereof

A liquid-based cell and preservation solution technology, applied in the preservation, application, animal husbandry and other directions of human or animal body, can solve the problems of unfavorable patient treatment, impossible to achieve, inconvenient liquid-based cell preservation solution, etc., to improve the accuracy of diagnosis. efficiency, improve work efficiency, and the effect of convenient operation

Inactive Publication Date: 2012-01-18
上海鎏轶生物技术中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since this liquid-based cell preservation solution is specially used in conjunction with the membrane-type liquid-based cell production machine, it is impossible to achieve the natural precipitation method to separate the mucus, blood and inflammatory cells in the specimen to achieve the accuracy of the smear. Higher quality and higher efficiency; and this liquid-based cell preservati

Method used

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  • Liquid-based cell preservation liquid composite and preparation method thereof
  • Liquid-based cell preservation liquid composite and preparation method thereof
  • Liquid-based cell preservation liquid composite and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Liquid-based cell preservation solution composition, comprising the following components by weight percentage:

[0030]

[0031] Wherein: described formaldehyde is analytical pure reagent, content 37%; Sodium diacetate is food additive grade; Sodium citrate is analytical pure reagent, sodium chloride is analytical pure reagent, NaCl>99.5%; EDTA is analytical pure reagent and Glacial acetic acid is analytically pure reagent, CH 2 COOH>99.5%, produced by Sinopharm Chemical Reagent Co., Ltd.

[0032] 20wt% of ethanol can also be added to the above components to speed up the action of fixing cells.

[0033] 0.01wt% of buffer potassium chloride and 0.2wt% of powder buffer sodium bicarbonate produced by Nanchang Baiyun Pharmaceutical Co., Ltd. can also be added in the above-mentioned buffer sodium chloride component, so that they form an efficient buffer system structure . This embodiment is easy to operate, improves the diagnostic accuracy, and can also keep the specim...

Embodiment 2

[0035] Liquid-based cell preservation solution composition, comprising the following components by weight percentage:

[0036]

[0037] Wherein: described formaldehyde is analytical pure reagent, content 40%; Sodium diacetate is food additive grade; Sodium citrate is analytical pure reagent, and sodium chloride is analytical pure reagent, NaCl>99.5%; EDTA is analytical pure reagent and Glacial acetic acid is analytically pure reagent, CH 2 COOH>99.5%, produced by Sinopharm Chemical Reagent Co., Ltd.

[0038] In the above components, 25 wt% of ethanol can also be added to speed up the action of fixing cells.

[0039] 0.02wt% of buffer potassium chloride and 0.4wt% of powder buffer sodium bicarbonate produced by Nanchang Baiyun Pharmaceutical Co., Ltd. can also be added in the above-mentioned buffer sodium chloride component, so that they form an efficient buffer system structure . This embodiment is easy to operate, improves the diagnostic accuracy, and can also keep the ...

Embodiment 3

[0041] Liquid-based cell preservation solution composition, comprising the following components by weight percentage:

[0042]

[0043] Wherein: described formaldehyde is analytical pure reagent, content 37%; Sodium diacetate is food additive grade; Sodium citrate is analytical pure reagent, sodium chloride is analytical pure reagent, NaCl>99.5%; EDTA is analytical pure reagent and Glacial acetic acid is analytically pure reagent, CH 2 COOH>99.5%, produced by Sinopharm Chemical Reagent Co., Ltd.

[0044] In the above components, 35 wt% ethanol can also be added to accelerate the action of fixing cells.

[0045] 0.02wt% of buffer potassium chloride and 0.4wt% of powder buffer sodium bicarbonate produced by Nanchang Baiyun Pharmaceutical Co., Ltd. can also be added in the above-mentioned buffer sodium chloride component, so that they form an efficient buffer system structure . The operation of this embodiment is convenient, allowing the cells to be observed to effectively ...

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Abstract

A liquid-based cell preservation liquid composite is characterized by comprising the following components in percentage by weight: cell fixing agent: 2-15% of formaldehyde, preservative: 0.1-5% of sodium diacetate, anticoagulant: 2-10% of sodium citrate, buffering agent: 0.5-2% of sodium chloride, chelating agent: 0.002-0.5% of ethylene diamine tetraacetic acid (EDTA), hemolytic agent: 0.5-10% ofglacial acetic acid, and water: 5-80% of pure water, wherein the formaldehyde is the analytical reagent, and accounts for 37-40%; the sodium diacetate is the food additives; the sodium citrate and the sodium chloride are analytical reagents, and NaCl is more than 99.5%; and the EDTA and the glacial acetic acid are analytical reagents, and H2COOH is more than 99.5%. The invention has the advantages that the liquid-based cell preservation liquid composite can keep the cell form to the maximum extent, can improve the diagnosis accuracy and can prevent the cell form of a specimen from significantdifference at room temperature for at least three months.

Description

technical field [0001] The invention relates to a reagent for liquid-based thin-layer cell detection, in particular to a liquid-based cell preservation solution composition and a preparation method thereof. Background technique [0002] In the field of liquid-based thin-layer cell detection technology, the fully automatic membrane-type liquid-based ultra-thin cell preparation system Xinpai TCT2000 processor and its supporting special reagent Cell Preservation Solution (PreserveCyt Solution) produced by American CCID Company are known as liquid The gold standard for cytology testing. Chinese Utility Model Patent No. 200620141836 also disclosed a membrane-type liquid-based cell sheeting machine on December 30, 2006, including a solution bottle for storing cell liquid base, a cell collection device and a controller for collecting cells in the solution bottle. The cell collection device includes a filter and a pressure supply device for providing pressure to the filter, the fil...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 张雪云杨丽思
Owner 上海鎏轶生物技术中心
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