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Application of light-emitting diode (LED) in plant tissue culture

A plant tissue culture and culture rack technology, applied in the field of new light sources, can solve problems such as no discovery, and achieve the effects of long life, high effective illumination, and improved space utilization.

Active Publication Date: 2013-04-10
SHUYANG COUNTY SUBEI FLOWER
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no application of LEDs as a light source for plant tissue culture has been found so far.

Method used

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  • Application of light-emitting diode (LED) in plant tissue culture
  • Application of light-emitting diode (LED) in plant tissue culture
  • Application of light-emitting diode (LED) in plant tissue culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Experimental materials

[0023] The chrysanthemum variety 'Xiangxue' was used in the experiment. Induce differentiation and culture into test tube seedlings according to the following method: choose the stem section of chrysanthemum as explant, take 1~2 internodes, inoculate in MS+6-BA2.0mg / L+NAA0.2mg / L+ On a solid medium of 30 g / L sucrose + 6 g / L agar (pH 5.8), differentiation was induced under conventional culture conditions (temperature 25° C., light intensity 3000 Lx, light 12 h / d). Transfer the differentiated seedlings to the solid medium of MS+6-BA1.0mg / L+NAA0.1mg / L+sucrose 30g / L+agar 6g / L (pH 5.8) for propagation, and transfer to MS+ after 30 days Sucrose 30g / L+agar 6g / L (pH 5.8) solid medium for further culture. After the seedlings were further cultivated for 30 days, the shoot tips with 3 leaves were taken as test materials.

[0024] In the experiment, the peeled seeds of Iris calamus were selected as explants, inoculated on MS solid medium, and induced t...

Embodiment 2

[0060] Such as figure 1 As shown, the lamp tube type LED culture rack used for plant tissue culture in the present invention includes a frame body 1, a plurality of partitions 2 are arranged on the frame body 1, and a plurality of lamp tube type LEDs 3 are arranged on the bottom surface of the partition board 2 ( figure 2 ), the tube-type LED 3 is connected to a control device 4 . The partition divides the culture rack into multiple layers to form a layered culture rack. This can provide more cultivation area in a limited space. The regulating device 2 regulates the light intensity of the LED by regulating the current or voltage, and can set the light cycle, thereby saving the cost of human participation. The regulating device 2 can individually control the LEDs of each layer, or control each LED individually. This saves unnecessary energy waste.

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Abstract

The invention provides the purposes of light-emitting diodes (LEDs) in plant tissue culture, and particularly relates to the application of LEDs which serve as plant tissue culture light sources. Through research, the invention also further determines that the utilization rate of the light source can be improved through the special ratio of red light and blue light, and the growth of plants can be effectively improved through special light intensity. The invention also provides a lamp tube-type LED culture rack for plant tissue culture, which comprises a rack body; a plurality of division boards are arranged on the rack body; a plurality of lamp tube-type LEDs are arranged on the bottom surfaces of the division boards; and the lamp tube-type LEDs are connected with a control device. The lamp tube-type LEDs which serve as the light sources can be widely applied to plant tissue culture, improve the growth efficiency of the plants and reduce the energy consumption.

Description

technical field [0001] The invention relates to a novel light source for plant tissue culture. Background technique [0002] Plant tissue culture is based on the theory that plant cells are totipotent, using isolated organs, tissues or cells of plants, such as roots, stems, leaves, flowers, fruits, seeds, embryos, ovules, ovaries, anthers, pollen and The parenchyma and vascular tissue of storage organs can induce callus, adventitious buds and adventitious roots under sterile and suitable artificial medium, light, temperature and other conditions, and finally form a complete plant. So far, tissue culture technology has been widely used in clone reproduction, flower breeding, plant virus removal and germplasm preservation all over the world. [0003] The light sources used in plant tissue culture are usually ordinary fluorescent lamps and high pressure sodium lamps. They generally have the disadvantages of short life, high heat generation and unsatisfactory luminous efficien...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04F21V23/04F21Y101/02
Inventor 高亦珂李萌莽宁宁张启翔程堂仁
Owner SHUYANG COUNTY SUBEI FLOWER
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