Mulberry leaf total alkali extract and preparation method and application thereof

Abstract

The invention discloses a mulberry leaf total alkali extract and a preparation method thereof. The method comprises the following specific steps of: (1) smashing mulberry leaves, and extracting with a solvent to obtain a mulberry leaf extracting solution; (2) concentrating the obtained mulberry leaf extracting solution into a concentrate of which relative density is 1.02-1.20, adding lime milk, shaking uniformly, adding ethanol for precipitating, centrifugally separating, and taking supernatant; and (3) adjusting the pH of the supernatant to 2.0-5.0, removing precipitates, adsorbing with macroporous cation exchange resin or gel-type cation exchange resin, washing with water, removing impurities, eluting the resin with ammonia water of which the mass concentration is 0.20-5.0 percent, collecting an ammonia water eluent, concentrating, and drying to obtain the mulberry leaf total alkali extract. Due to the adoption of the preparation method, the service life of the cation exchange resinis prolonged, and the production cost is saved. The invention further discloses application of the mulberry leaf total alkali extract prepared with the method to the preparation of a medicament or a health-care product or a food for treating or preventing diabetes mellitus and complications thereof.

Description

technical field [0001] The invention relates to a total alkaloid extract of mulberry leaves extracted from the leaves of Moraceae Morus plants and a preparation method thereof, and the total alkaloid extract of mulberry leaves is used to prepare a medicine or a key for treating or preventing diabetes and its complications products or food applications. Background technique [0002] The mulberry leaf (Morus alba L.) of the genus Moraceae is the part traditionally used as medicine. Commonly used in folk medicine includes Morus australis Poir., M.mongolica Schnoid., M. bombycis koidz., and M. mulberry .notabilis Schne., Hua Sang M.cathayana Hensl., etc. A large number of studies at home and abroad have shown that mulberry leaves have a good hypoglycemic function, and the mechanism of its hypoglycemic effect has been shown to be closely related to the azasaccharide alkaloids. [0003] Mulberry leaves mainly contain flavonoids and their derivatives, coumarin derivatives, triter...

AI Technical Summary

Problems solved by technology

Although these methods can increase the relative content of DNJ in mulberry leaves, there are defects such as complicated procedures, repeated columns, desalination treatment, long production cycle, and relatively high cost.

[0005] The molecular weight of mulberry leaf polyhydroxyalkaloids is about 150-300 Daltons, and the water solubility is excellent. The current desalination method is difficult to apply to the desalination of mulberry leaf polyhydroxyalkaloids: ① The ion exchange method needs to pass through the column again, and the process Complex, affecting yield, and difficult to remove Na+; ②Electrodialysis or reverse osmosis is mainly used for desalination from water, not suitable for desalination from polar mulberry leaf alkaloid molecules with good water solubility; ③For protein desalination Dialysis or gel method is suitable for the desalination of larger molecular weight substances, but its industrialization cost is high and cannot be used for mulberry leaf alkaloids

[0006] Although the ion exchange resin method has been used in the prior art to extract the total alkaloids of mulberry leaves, the one-sided purification of 1-deoxynojirimycin (DNJ) is easy to ignore the use cost of the resin, such as after the acidification solution is concentrated in CN200710067498.3 Directly use ion exchange resin column chromatography for separation, although the cost of flocculant is saved, but the service cycle of ion exchange resin column is seriously shortened and the production cost is increased

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