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Method for producing fodder rhodotorula through mixed solid fermentation

A technology of solid fermentation and red yeast, applied in the field of microbial fermentation, can solve the problems of high cost of culture materials and low fermentation level, and achieve the effects of being beneficial to environmental protection, improving fermentation level and reducing production cost

Active Publication Date: 2013-02-06
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a low-cost, easily available agricultural and sideline product as raw material, supplemented by a reasonable fermentation process, in view of the defects of high cost of culture raw materials and low fermentation level in the current fermentation production of red yeast. Method for producing red yeast for feed by mixed solid fermentation to improve fermentation level and reduce production cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: (mixed solid fermentation produces the method 1 of red yeast for feeding)

[0018] The inventive method carries out as follows:

[0019] (1) Seed liquid culture of Rhodotorula glutinis strain 2.499: insert the original slant strain of Rhodotorula glutinis strain 2.499 into the culture medium of the Erlenmeyer flask, the medium loading capacity of 300ml Erlenmeyer flask is 50ml, after 26~28°C, 180 ~200rpm shaker cultured for 18~24 hours to become the seed liquid of the triangular flask, and set aside; the seed culture medium of the triangular flask is: 10°Bx saccharified wort added with 0.5% peptone, adjusted to pH 6.0~6.5, and sterilized at 121°C for 15 minutes;

[0020] (2) seed liquid cultivation of Mucor Rouxianus (Mucor Rouxianus) strain 3.3447: Mucor Rouxianus strain 3.3447 slant mother species is inserted in the 6% bran juice medium of the triangular flask, and the amount of culture medium in the 300ml triangular flask is 50ml, After 26~28 ℃, 200~22...

Embodiment 2

[0023] Embodiment 2: (mixed solid fermentation produces the method 2 of red yeast for feeding)

[0024] In this example, the preparation of step (3) red yeast and mucormyces ruckeri triangular flask solid mixed strains: first bran, soybean meal and ammonium sulfate are mixed in a ratio of 70: 30: 0.8 by weight, and mixed by weight of the solids Add 1.1 times of water, mix well, form solid medium after 0.1Mpa sterilization for 30min, and put it into 500ml Erlenmeyer flasks according to 25g / bottle; then insert step (1), (2) 1ml of each seed solution, mixed evenly, cultured at 26-28°C for 72 hours, stored at 0-4°C, and set aside;

[0025] Step (4) Mixed solid fermentation culture of red yeast: mix by weight bran: soybean meal: ammonium sulfate = 60: 40: 1, add water 1.0 times the weight of solids and 0.1Mpa sterilization for 30 minutes to prepare solid culture base; the solid mixed bacterial strains in the triangular flask of step (3) are poured out, first add 300ml sterile wate...

Embodiment 3

[0027] Embodiment 3: (the method 3 of mixed solid fermentation production red yeast for feed)

[0028]In this example, the preparation of step (3) red yeast and mucormyces ruckeri triangular flask solid mixed bacterial strains: first bran, soybean meal and ammonium sulfate are mixed in the ratio of 80:20:0.5 in parts by weight, and mixed according to the weight of the solids Add 1.2 times of water, mix well, form solid culture medium after 0.1Mpa sterilization for 30min, and put it into 500ml triangular flasks according to 30g / bottle; then insert step (1), (2) 1ml of each seed solution, mixed evenly, cultured at 26-28°C for 72 hours, stored at 0-4°C, and set aside;

[0029] Step (4) Mixed solid fermentation culture of red yeast: mix by weight bran: soybean meal: ammonium sulfate = 70: 30: 0.8, add water 1.1 times the weight of solids and 0.1Mpa sterilization for 30 minutes to prepare solid culture Base; pour out the solid mixed bacterial strains in the triangular flask of ste...

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PUM

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Abstract

The invention discloses a method for producing fodder rhodotorula glutinis through mixed solid fermentation, which belongs to the technical field of microbial fermentation. The method comprises the following steps and processes: (1), the culture of a seed liquid of rhodotorula glutinis, (2), the culture of a seed liquid of mucor rouxianus, (3), the preparation of solid mixed strains through a triangular flask, (4), the mixed solid fermentation and culture of the rhodotorula glutinis, and the like. According to the method, a macromolecular material is decomposed into a micromolecular substance by utilizing the mucor rouxianus, so that the rhodotorula glutinis grows efficiently; the digestive absorption of livestock to nutrient substances is also promoted by utilizing a bioactive substance generated by the mucor rouxianus; and the method has the characteristics that the environment is not polluted, the production cost of a product is low, the fermentation level is high, the service effect is good, and the like. The method can be popularized and applied in fodder rhodotorula glutinis producing enterprises and livestock and aquatic product enterprises.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a method for producing red yeast for feed by a mixed solid fermentation process of Mucor rousei and rhodotorula. technical background [0002] Red yeast is not only rich in protein and vitamins, but also rich in astaxanthin. Astaxanthin can improve the immunity and anti-inflammatory ability of animals, thereby greatly improving the survival rate and significantly reducing infectious diseases. It can also increase fertility and increase the egg production rate of poultry; Special color of muscle; poultry will enrich astaxanthin in eggs, which not only makes the color of egg yolk darker, but also greatly improves the nutritional value of eggs. Astaxanthin is stronger than the well-known β-carotene in terms of promoting antibody production, enhancing host immune function, and quenching free radicals. In addition, the antioxidant capacity of astaxanthin is 10 times h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12N1/14C12R1/645C12R1/785
Inventor 李孝辉皮雄娥
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES