A human pancreatic cancer cell line resistant to gemcitabine and its application

A technology of pancreatic cancer cells and gemcitabine, applied in the field of cell lines, can solve the problems of low biodiversity, insufficient gemcitabine drug resistance, large differences in biological characteristics of pancreatic cancer, etc., and achieve the effect of stable characteristics

Active Publication Date: 2015-08-19
开拓者医学研究(上海)有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a new human pancreatic cancer cell line with low biological diversity, a large difference from the clinical biological characteristics of pancreatic cancer, and serious drug re

Method used

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  • A human pancreatic cancer cell line resistant to gemcitabine and its application
  • A human pancreatic cancer cell line resistant to gemcitabine and its application
  • A human pancreatic cancer cell line resistant to gemcitabine and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0037] The preparation of embodiment 1PAXC-002 cell

[0038] Nude mice: 5 nude mice, female, weighing 16.0±1.0 g, aged 5 weeks, raised in SPF environment. Nude mice were provided by Shanghai Slack Experimental Animal Technology Co., Ltd.

[0039] A fresh resection specimen of clinical pancreatic cancer (female, 56 years old, obstructive jaundice: carcinoma of the head of the pancreas, pathological diagnosis: poorly to moderately differentiated ductal adenocarcinoma of the head of the pancreas) was obtained from Shanghai Changhai Hospital, and immediately immersed in a pre-cooled sterile bacteria HBSS buffer (containing 500U / ml penicillin G, 500μg / ml streptomycin sulfate and 1.25μg / ml amphotericin B). In a biosafety cabinet, rinse the specimen with fresh sterile HBSS buffer, cut into small pieces of 20-50 mg, and puncture and inoculate subcutaneously in the axillary back of nude mice. After inoculation, the animals were in good health and behaved normally. 40 days after inoc...

Embodiment 2

[0043] Biological characteristics and application of embodiment 2PAXC-002 cells

[0044] The invention adopts RPMI 1640 culture solution containing fetal bovine serum and insulin to cultivate PAXC-002 cells, so that they can grow long-term in vitro and pass stably. When the cells are passed to more than 20 generations, the cell properties are gradually stabilized, and the relevant biology, genetics and tissue source identification are carried out until the 50th generation has the same stable properties. Through experimental observation and verification, PAXC-002 cells grown in vitro have a typical epithelial-like morphology, lose contact growth inhibition, and show malignant growth. Genetic studies have confirmed that the cells are aneuploid, with severe chromosome number and structural aberrations, consistent with the genetic characteristics of malignant tumors. The PAXC-002 cells can form tumors in nude mice and have tumorigenicity. The corresponding relationship between t...

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Abstract

The invention provides a primary gemcitabine-tolerant human pancreatic cancer cell, and a preparation method and application thereof. The human pancreatic cancer cell is collected in China Center for Type Culture Collection, and the collection number is CCTCC NO:C201010. The human pancreatic cancer cell can be used for generating human pancreatic cancer in mammals, and preparing a human pancreatic cancer model; and the human pancreatic cancer cell has high tolerance to the first-grade drug (gemcitabine) for treating pancreatic cancer, belongs to primary drug tolerance, and can be used for researching the pancreatic cancer drug-tolerant molecular mechanism and screening candidate drugs for treating drug-tolerant human pancreatic cancer. The human pancreatic cancer cell line has the advantage of stable characters and can be used for stable passage many times. The in-vitro passage characters from the 20th generation to the 50th generation remain stable. The human pancreatic cancer cell line provided by the invention has biological characters of human pancreatic cancer in clinic, and provides a new experimental material, which is more approximate to biological characters of tumors in clinic, for research of pancreatic cancer.

Description

technical field [0001] The invention belongs to the field of cell lines, in particular to a gemcitabine-resistant human pancreatic cancer cell line and its application. Background technique [0002] Pancreatic cancer is a malignant tumor with insidious onset, rapid progression and poor prognosis. In Europe, there were 60,000 new cases of pancreatic cancer and 59,000 deaths in 2009, ranking fifth in the mortality rate of malignant tumors in Europe; in the United States, the annual number of new cases of pancreatic cancer was about 42,470, ranking eighth, but The number of deaths from pancreatic cancer was 35,240, and it has become the third cause of death among malignant tumors. In my country, pancreatic cancer is one of the three major causes of death from malignant tumors of the digestive tract, and its case fatality rate is increasing year by year. [0003] Pancreatic cancer is not easy to detect at an early stage. No specific clinical manifestations and tumor markers have...

Claims

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Application Information

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IPC IPC(8): C12N5/09A01K67/027A61K49/00C12R1/91
Inventor 秦宵然朱明华胡刚谢付波汤旭蓁闻丹忆
Owner 开拓者医学研究(上海)有限公司
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