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Chloramphenicol molecular imprinting polymer microballoon with uniformity in size as well as preparation method and application thereof

A technology of molecular imprinting and polymers, applied in alkali metal compounds, chemical instruments and methods, alkali metal oxides/hydroxides, etc., can solve problems such as tailing, low resolution, and broadening of chromatographic peaks

Inactive Publication Date: 2013-11-06
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, there have been literature reports on molecularly imprinted polymers of chloramphenicol, which are mainly prepared by two methods: first, solution polymerization or bulk polymerization is used, and the molecularly imprinted polymers of chloramphenicol are blocky and have low resolution , and the adsorption efficiency is low, and when used as a chromatographic packing, it is easy to cause broadening of the chromatographic peak and severe tailing; secondly, the suspension polymerization method is used to prepare molecularly imprinted polymer microspheres with better spherical shape, but the obtained chloramphenicol The particle size uniformity of molecular polymer microspheres is poor, usually the coefficient of variation CV>35%, which affects the selective adsorption effect, and when used in chromatographic media, it needs to be sieved to obtain microspheres with a certain particle size range, resulting in waste

Method used

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  • Chloramphenicol molecular imprinting polymer microballoon with uniformity in size as well as preparation method and application thereof
  • Chloramphenicol molecular imprinting polymer microballoon with uniformity in size as well as preparation method and application thereof

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preparation example Construction

[0035] Regarding the preparation of molecularly imprinted polymer microspheres, there are many factors that affect the performance of molecularly imprinted polymers, including: template molecule dosage, functional monomer, crosslinking agent, type and dosage of porogen, polymerization temperature, time, and initiator dosage Wait. Therefore, molecularly imprinted polymer microspheres need to investigate multiple conditions.

[0036] The preparation method of the blank polymer used for the control, except that the template molecule chloramphenicol is not added, other conditions are the same as the preparation conditions of the molecularly imprinted polymer microspheres.

Embodiment 1

[0038] First, prepare the dispersed phase solution. Dissolve 1mmol of template molecule CDHB ultrasonically in 5mL of porogen chloroform and ethyl acetate (weight ratio 1:1), add 4mmol of functional monomer 4-vinylpyridine, crosslinker EDMA 19mmol, Initiator azobisisobutyronitrile AIBN 0.20g, the solution is degassed by ultrasonic for 5min, nitrogen is deaerated for 10min, and then the solution is transferred to the syringe of the syringe pump (try to avoid air entering the syringe); prepare a continuous phase solution and disperse Add 0.72 g of PVA to 60 mL of water, stir to dissolve, ultrasonically degas for 5 min, bubbling in nitrogen for 20 min, and place it in a continuous phase container.

[0039] The continuous phase was stirred at 210 rpm and nitrogen gas was introduced. Driven by the syringe pump, the dispersed phase solution enters the square chamber of the microfluidic device through the connecting tube, and then enters the continuous phase through the microchannel, an...

Embodiment 2

[0044] First, prepare the dispersed phase solution. Dissolve 1mmol template molecule CDHB ultrasonically in 6mL of porogen chloroform and ethyl acetate (weight ratio 4:6), add 3mmol functional monomer allylpiperazine, crosslinker EDMA 18mmol, Initiator azobisisobutyronitrile AIBN 0.22g, the solution is degassed by ultrasonic for 5min, nitrogen is deaerated for 10min, and then the solution is transferred to the syringe of the syringe pump (try to avoid air entering the syringe); prepare a continuous phase solution and disperse Add 0.78 g of PVA to 66 mL of water, stir to dissolve, ultrasonically degas for 5 min, bubbling in nitrogen for deoxygenation for 20 min, and place in a continuous phase container.

[0045] The continuous phase was stirred at 230 rpm and nitrogen gas was introduced. Driven by the syringe pump, the dispersed phase solution enters the square chamber of the microfluidic device through the connecting tube, and then enters the continuous phase through the microch...

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Abstract

The invention discloses a chloramphenicol molecular imprinting polymer microballoon which has mean grain size of 1mum to 300mum and grain size distribution coefficient C.V of not more than 15 percent. The preparation method of the chloramphenicol molecular imprinting polymer microballoon comprises the following steps of: firstly, preparing emulsion by using a micro-fluidic device; secondly, curing the emulsion to obtain a molecular imprinting microballoon, wherein the micro-fluidic device comprises a plurality of micro channels; generating the emulsion when a dispersion phase is driven by an injection pump to enter a continuous phase from the micro channels; transferring the emulsion to another container and curing to obtain a polymer microballon; eluting the polymer microballon to remove a template molecule; and obtaining the molecular imprinting polymer microballoon. The dispersion phase contains the template molecule of chloramphenicol, a functional monomer, a cross linker, an evocating agent and a pore-foaming agent; and the continuous phase contains water and a dispersion agent. The invention also provides application of the chloramphenicol molecular imprinting microballoon.

Description

Technical field [0001] The invention relates to the fields of functional polymer materials and biochemical separation and analytical chemistry. More specifically, it relates to a chloramphenicol molecularly imprinted polymer microsphere with uniform size. [0002] The invention also relates to a preparation method of the molecularly imprinted polymer microspheres. [0003] The invention also relates to the application of the molecularly imprinted polymer microspheres. Background technique [0004] As people's demand for animal foods increases, the issue of veterinary drug residues in animal foods has increasingly become the focus of the whole society. In order to protect the health of our people and effectively control the amount of veterinary drug residues in edible animal products, it is necessary to vigorously carry out the detection of trace veterinary drug residues in animal-derived products. [0005] Chloramphenicol (CAP) is a broad-spectrum antibiotic with strong bactericidal...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F222/14C08F226/06C08F220/06C08F212/36C08F220/56C08F220/28C08F2/22C08J9/26B01J20/285B01J20/30B01J20/28
Inventor 雷建都马光辉苏志国寇星翟艳琴
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI