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Complex microbial inoculum for efficiently ungluing bast fiber and preparation method for complex microbial inoculum

A technology of composite bacterial agent and bast fiber, which is applied in fiber treatment, chemical post-treatment of fiber, methods based on microorganisms, etc., can solve the problems of large differences in cultured bacteria, unstable product quality, and long time consumption, etc., to achieve High economic prospects and social and environmental benefits, rich organic matter content in wastewater, and good fiber spinnability

Inactive Publication Date: 2012-02-01
XIHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The natural retting degumming method has the following limitations: it takes a long time, occupies a large area, uses a lot of water, and is not easy for sewage treatment. It usually takes more than ten days or even longer, and because of the changeable external natural conditions and seasonal changes, it is difficult to do it artificially. Therefore, the product quality is extremely unstable; the retting process requires a large number of pools and clear water; the large amount of sewage generated is difficult to centrally recycle and treat, so it cannot meet the needs of modern industrial production;
The new microbial degumming method of artificial inoculation of degumming bacteria has attracted much attention in recent years. There have been many research articles published in this area at home and abroad, but most of them cultivate a single bacterium by themselves and inoculate it on the bast fiber for degumming. This degumming method Due to the single species of bacteria, the difference in self-cultured bacteria solution is large, and it cannot be recycled, so the actual effect is not satisfactory

Method used

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Embodiment Construction

[0011] The present invention will be described in detail below in conjunction with specific embodiments.

[0012] The invention provides a bast fiber high-efficiency degumming composite bacterial agent and a preparation method thereof.

[0013] First, Bacillus amyloliquefaciens subsp. amyloliquefaciens DSM 7 with a seed age of 16 to 20 hours (cultivated according to the medium and method recommended at the time of purchase) T , strain number DSM7 T , German Culture Collection of Microorganisms,) and Bacillus subtilis subsp.inaquosorum BGSC 3A28 T , strain number BGSC 3A28 T , American Bacteria Genetic Atlas Center), the two suspensions were mixed in a ratio of 3:2 by volume, and the mixed solution was inoculated in the following medium for co-cultivation: soybean meal 1.0-1.2% (W / V is the volume of material quality / water, hereinafter Unless otherwise specified are W / V), (NH 4 ) 2 HPO 4 0.1%, MgSO 4 ·7H 2 O 0.05%, corn steep liquor 0.05%, pH7~8, sterile air 1dm 3 / min...

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Abstract

The invention discloses a complex microbial inoculum for efficiently ungluing a bast fiber. A preparation method for the complex microbial inoculums comprises the following steps of: mixing a Bacillus amyloliquefaciens subsp. amyloliquefaciens DSM 7<T> suspension of which the age is 16-20 hours and a Bacillus subtilis subsp. Inaquosorum BGSC 3A28<T> suspension at the volume ratio of 3: 2; inoculating the mixed liquor into the following culture medium for joint culturing for 14-16 hours; filtering the culture solution through a 80-mesh filter; adding 12-18%(W / V similarly hereinafter) of porousstarch, 12-18% of beta-cyclodextrin, 0.1% of thiourea and 0.1% of povidone in the filtrate; and drying through spraying and adding tricalcium phosphate the weight of which is 5% of the total weight of the materials as a filler and vacuumizing to obtain the complex microbial inoculums. By using the complex microbial inoculums to unglue the bast fiber, the production cost is reduced, the product quality is improved, and the discharge and treatment of the high alkali wastewater are reduced radically and the complex microbial inoculum has a good economic prospect and high environmental benefit.

Description

technical field [0001] The invention relates to a composite bacterial agent for highly efficient degumming of bast fibers and a preparation method thereof. Background technique [0002] Bast fiber is a general term for fibers in the outer bast of woody plant stems, and is a natural green plant fiber. The bast of woody plants is mainly composed of pectin, hemicellulose, cellulose and lignin according to a certain biological structure, so separating cellulose from bast is the most basic and critical process for plant bast fiber processing , is a major research topic in the natural plant textile industry. [0003] Separation of fibers from plant bast, known in the textile industry as "degumming". The most common degumming methods include chemical degumming and biological degumming. At present, the degumming of bast fibers in industry is still dominated by chemical degumming centered on caustic soda smelting. Chemical degumming consumes a lot of water, chemical raw materials ...

Claims

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Application Information

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IPC IPC(8): C12N1/20D01C1/00C12R1/07C12R1/125
Inventor 向文良车振明李明元唐洁
Owner XIHUA UNIV
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