Complex microbial inoculum for efficiently ungluing bast fiber and preparation method for complex microbial inoculum
A technology of composite bacterial agent and bast fiber, which is applied in fiber treatment, chemical post-treatment of fiber, methods based on microorganisms, etc., can solve the problems of large differences in cultured bacteria, unstable product quality, and long time consumption, etc., to achieve High economic prospects and social and environmental benefits, rich organic matter content in wastewater, and good fiber spinnability
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[0011] The present invention will be described in detail below in conjunction with specific embodiments.
[0012] The invention provides a high-efficiency degumming composite bacterial agent for bast fibers and a preparation method thereof.
[0013] First, Bacillus amyloliquefaciens subsp.amyloliquefaciens DSM 7 with a seed age of 16-20h (all cultivated according to the recommended medium and method at the time of purchase) T , Strain number DSM7 T , German Microbial Culture Collection,) and Bacillus subtilis subsp.inaquosorum BGSC 3A28 T , Strain number BGSC 3A28 T , American Bacterial Genetic Atlas Center), the two suspensions were mixed in a volume ratio of 3:2, and the mixture was inoculated in the following medium for co-cultivation: soybean meal 1.0~1.2% (W / V is the mass of material / volume of water, below Unless otherwise specified are W / V), (NH 4 ) 2 HPO 4 0.1%, MgSO 4 ·7H 2 O 0.05%, corn steep liquor 0.05%, pH 7~8, sterile air 1dm 3 / min·L, temperature 38℃~40℃, incubate for...
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