Complex microbial inoculum for efficiently ungluing bast fiber and preparation method for complex microbial inoculum
A technology of composite bacterial agent and bast fiber, which is applied in fiber treatment, chemical post-treatment of fiber, methods based on microorganisms, etc., can solve the problems of large differences in cultured bacteria, unstable product quality, and long time consumption, etc., to achieve High economic prospects and social and environmental benefits, rich organic matter content in wastewater, and good fiber spinnability
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[0011] The present invention will be described in detail below in conjunction with specific embodiments.
[0012] The invention provides a bast fiber high-efficiency degumming composite bacterial agent and a preparation method thereof.
[0013] First, Bacillus amyloliquefaciens subsp. amyloliquefaciens DSM 7 with a seed age of 16 to 20 hours (cultivated according to the medium and method recommended at the time of purchase) T , strain number DSM7 T , German Culture Collection of Microorganisms,) and Bacillus subtilis subsp.inaquosorum BGSC 3A28 T , strain number BGSC 3A28 T , American Bacteria Genetic Atlas Center), the two suspensions were mixed in a ratio of 3:2 by volume, and the mixed solution was inoculated in the following medium for co-cultivation: soybean meal 1.0-1.2% (W / V is the volume of material quality / water, hereinafter Unless otherwise specified are W / V), (NH 4 ) 2 HPO 4 0.1%, MgSO 4 ·7H 2 O 0.05%, corn steep liquor 0.05%, pH7~8, sterile air 1dm 3 / min...
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