Berberine-phenylacetic acid derivative, pharmaceutically acceptable salt thereof and application thereof
A derivative, berberine technology, applied in the field of berberine-phenylacetic acid derivatives and pharmaceutically acceptable salts, can solve problems such as difficulty in dissolution and absorption, liver first-pass effect, increased toxicity, etc. Efficacy, reduce drug toxicity, enhance the effect of drug targeting
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Embodiment 1
[0039] Example 1: In vitro hypoglycemic activity experiment
[0040] 1. Experimental materials and methods
[0041] 1 Instruments and reagents
[0042] 1.1 Main experimental instruments
[0043] (1) SW-CJ-2F double-sided double-sided ultra-clean workbench (Suzhou Purification Equipment Co., Ltd.)
[0044] (2) MCO-150A carbon dioxide incubator (Japan SANYO company)
[0045] (3) Inverted Phase Contrast Microscope (Olympus CK40-F200)
[0046] (4) Automatic balance centrifuge (Reboer LDZ 4-1.2)
[0047] (5) 25cm2 culture flask, 48, 96-well culture plate (COSTAR, USA)
[0048] (6) SHH-W21-600 three-purpose electric heating constant temperature water tank (Tianjin Test Instrument Co., Ltd.)
[0049] (7) KQ-100DE CNC ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.)
[0050] (8) MLS-3020 high-pressure steam sterilizer (Japan SANYO company)
[0051] (9) DGX-9243B-2 blast drying oven (Shanghai Fuma Experimental Equipment Co., Ltd.)
[0052] (10) SN-3001 Microplate R...
Embodiment 2
[0093] Embodiment 2: the preparation method of the invented compound 1d tablet
[0094] prescription:
[0095]
[0096] Preparation method: prepare 4% propyl methylcellulose (E-30) solution, set aside. Weigh 10 g of starch and dry at 105°C for 5 hours for later use. Weigh 20g of starch and the invented compound 1d and microcrystalline cellulose of the prescribed amount, mix well, and pulverize through an 80-mesh sieve. Use 4% propyl methylcellulose (E-30) solution to make the material into soft material, granulate it with a 20-mesh sieve, and dry it at 50°C to 60°C until the water content in the granule is about 3%. Pass through a 20-mesh sieve for granulation, add dry starch (dried at 105°C for 5 hours) and magnesium stearate in the prescribed amount, and finally mix, measure the content of intermediates, and determine the tablet weight; tablet.
[0097] Usage and dosage: According to the patient's condition and individual differences, it is recommended to take 3-6 tabl...
Embodiment 3
[0098] Embodiment 3: the preparation method of invented compound 2i capsule
[0099] prescription:
[0100]
[0101] Preparation method: After the synthesized compound 2i is micronized and pulverized into very fine powder, it is uniformly mixed with auxiliary materials according to the above-mentioned formula ratio, and the soft material is made with water level wetting agent, and after being made into 12-14 mesh particles by a granulator, the Dry at 40-50°C; sieve to remove fine powder, and the finished product has uniform particles and consistent color. No moisture absorption caking. After the routine inspection of the Pharmacopoeia, it meets the relevant regulations and is qualified, and commercially available No. 3-5 capsules can be selected. After loading and sealing, the surface of the capsules is polished and printed, and the capsules can be prepared, bottled and packaged after checking the difference in filling volume and disintegration time limit according to the...
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