Method for measuring content of substance in process of anaerobic fermentation

An anaerobic fermentation and material technology, applied in biochemical equipment and methods, measuring devices, anaerobic digestion treatment, etc., can solve the problems of long separation time, low accuracy and poor reproducibility of column chromatography and paper chromatography , to save analysis time and cost

Inactive Publication Date: 2012-02-08
UNIV OF SCI & TECH OF CHINA
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Problems solved by technology

However, these methods have certain limitations, such as colorimetry can only measure a single acid, column chromatography and paper chromatography have long separation time, low accuracy, and poor reproducibility, titration can only measure mixed acids, and gas chromatography requires Carry out acidification and filtration pretreatment (Lahav et al.Environ.Sci.Technol.2002, 36, 2736)

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  • Method for measuring content of substance in process of anaerobic fermentation
  • Method for measuring content of substance in process of anaerobic fermentation
  • Method for measuring content of substance in process of anaerobic fermentation

Examples

Experimental program
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Embodiment 1

[0025] The mensuration of sucrose in embodiment 1, anaerobic fermentation process

[0026] 1. With the progress of the fermentation time, a total of 100 samples were taken. The fermentation liquid was centrifuged at 12000r / min for 5min when sampling, and 20ml of the upper clear liquid was taken and poured into a glass tube. The transmission fiber probe is used for scanning measurement to obtain near-infrared spectrum signals. Near-infrared test range from 5500cm -1 -10000cm -1 , the sampling interval is 3.856cm -1 (1168 data points). The resolution of the instrument is 4.0cm -1 . Each spectrum is the average result of 64 scans. Each sample was repeated twice, and the average spectrum was taken. After each sample is measured, the fiber optic probe is immediately cleaned with distilled water, and the next sample is measured after drying.

[0027] Scan results such as figure 1 shown.

[0028] 2. Utilize prior art to measure the content of the sucrose in the fermented li...

Embodiment 2

[0049] The mensuration of ethanol in embodiment 2, anaerobic fermentation process

[0050] 1. The scanning steps of the near-infrared spectrometer are the same as step 1 in Example 1, and the results are as follows figure 1 shown.

[0051] 2. Utilize prior art to measure the content of the ethanol in the fermented liquid of step 1

[0052] Measured by gas chromatography, ethanol and acetic acid, propionic acid and butyric acid in the following examples are all quantified by external standard method.

[0053]The fermented liquid is pretreated to obtain the filtrate before the gas phase chromatograph, and the method is as follows: take 1.0 ml of each fermented liquid in step 1, dilute and acidify with 4.0 ml of 3% formic acid solution, and centrifuge at 10,000 rpm with a centrifuge After 5 minutes, finally take the supernatant and filter it with a 0.45 μm filter membrane to obtain a filtrate.

[0054] Then take the filtrate and analyze it in the automatic sampling system of t...

Embodiment 3

[0061] The mensuration of acetic acid in embodiment 3, anaerobic fermentation process

[0062] 1. The scanning steps of the near-infrared spectrometer are the same as step 1 in Example 1, and the results are as follows figure 1 shown.

[0063] 2. Utilize prior art to measure the content of the acetic acid in the fermented liquid of step 1, step is the same as example 2 step 2.

[0064] 3. Establish a near-infrared spectrum multivariate correction model about the near-infrared spectrum signal and acetic acid content, the steps are the same as the example 2 step 3, and the final near-infrared spectrum multivariate correction model is as follows image 3 Shown in acetic acid.

[0065] 4. Using the near-infrared spectrometer again to scan and measure the fermentation broth to be tested, to obtain near-infrared spectrum signals.

[0066] The result is as Figure 4 shown.

[0067] 5, obtain the content of acetic acid ( Figure 5 ). The prediction effect of the model on unknow...

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Abstract

The invention discloses a method for measuring the content of substance in a process of anaerobic fermentation, comprising the steps of: 1) scanning fermentation liquor in the process of anaerobic fermentation by a near-infrared spectrometer, and obtaining a near-infrared spectrum signal; 2) measuring the content of the substance to be measured in the fermentation liquor of the step 1) by the existing technology; 3) building a near-infrared spectrum multivariate calibration model about the near-infrared spectrum signal and the content of the substance to be measured; 4) scanning the fermentation liquor in the process of the anaerobic fermentation by the near-infrared spectrometer again, and obtaining another near-infrared spectrum signal; and 5) obtaining the content of the substance in the process of the anaerobic fermentation according to the near-infrared spectrum multivariate calibration model and the near-infrared spectrum signal obtained in the step 4). After the method disclosed by the invention is adopted, the simple pretreatment is only needed to be carried out for a sample; and compared with the traditional method, a great deal of analysis time and expense can be saved, so that a good tool is provided for monitoring the fermentation process of anaerobic reaction.

Description

technical field [0001] The invention relates to a method for measuring the content of intermediate products and substrates in the anaerobic fermentation process, in particular to a quantitative determination method for acetic acid, propionic acid, butyric acid and ethanol in volatile fatty acids in liquid phase products in the anaerobic fermentation process. Background technique [0002] Volatile fatty acids (VFAs) are important anaerobic fermentation intermediates during anaerobic biological treatment of wastewater, so the degradation of organic matter can be well understood by monitoring the changes of VFAs during anaerobic fermentation of wastewater process and the activity of methanogens and the operation of the system (Lahav et al. Environ. Sci. Technol. 2002, 36, 2736). The traditional VFA determination methods mainly include colorimetry, column chromatography, paper chromatography, titration and the commonly used gas chromatography. However, these methods have certai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/35G01N1/28C02F3/28G01N21/3577G01N21/359
CPCC12M41/32
Inventor 盛国平张梦霖俞汉青
Owner UNIV OF SCI & TECH OF CHINA
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