Method for constructing uric acid sensor on the basis of super-long zinc oxide nano-wire

Abstract

The invention provides a method for constructing a uric acid sensor on the basis of a super-long zinc oxide nano-wire. The method sequentially comprises the steps of: first preparing a super-long zinc oxide nano-wire; then evaporating a layer of Ti / Au electrodes on an insulating silicon wafer; bridging the electrodes with the nano-wire, which is fixed by silver paste, and leading out a copper lead simultaneously; etching impurities on the surface of the zinc oxide nano-wire with oxygen plasma, and fixing a layer of bio-enzyme by a chemical modification method; finally adjusting the bovine serum albumin (BSA) concentration in the reaction solution, thus realizing uric acid molecule detection according to concentration from low to high. The main points of the invention lie in that: the method provided in the invention has no need for a catalyst of Au and other precious metals and also has no need for using a vacuum pump to maintain a reaction chamber in a low pressure state; and the uric acid field effect transistor sensor constructed by the super-long zinc oxide nano-wire in the invention has low cost and large detection scope, thus being suitable for actual human body uric acid concentration detection.

Description

technical field [0001] The invention belongs to the field of preparation and application of one-dimensional nanowires, and in particular provides a method for preparing ultra-long zinc oxide nanowires. Uniform zinc oxide nanowires are grown on catalyst-free silicon wafers under high concentrations of reactants , and then used this ultra-long ZnO nanowire to construct a uric acid field effect transistor sensor. Background technique [0002] ZnO is a group II-VI compound and is a wide bandgap direct bandgap semiconductor material with a bandgap width of 3.37 eV at room temperature. It has the characteristics of high melting point and thermal conductivity, good chemical stability, high specific surface area, electrochemical activity, high isoelectric point and good biocompatibility, suitable for mass adsorption of proteins or enzymes with low isoelectric point, and for immobilization The enzyme provides a good microenvironment, which can well maintain the activity and stabili...

AI Technical Summary

Problems solved by technology

[0003] At present, researchers in the world mainly use one-dimensional nanowires to construct field-effect transistor nanobiosensors, but there are at least four deficiencies: First, because ZnO nanomaterials are soluble in liquids such as water and alcohol, there are few reports using ZnO Nanomaterials are used as biosensors, so research on their performance is relatively lacking; second, the prepared nanowires are very short, so the corresponding FET channels are also relatively short; third, the concentration detection range of biomolecules is very small; fourth, The curve does not increase (or decrease) in steps every time the concentration of biomolecules increases, so it is not practical

For example, the detection range of the biosensor based on one-dimensional ZnO nanowires reported by Korean scientist Sang Yeol Lee is only limited to 2.5nM-250nM, and when the concentration increases each time, there is no obvious step curve.

For the actual human uric acid concentration detection, such a curve that cannot distinguish the concentration cannot be applied, so this device still needs to be improved.

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