Pharmaceutical application of imperatorin in treatment of hypertension complicated with myocardial hypertrophy and heart protection
A technology for imperatorin and cardiac hypertrophy, which can be used in drug combinations, active ingredients of heterocyclic compounds, cardiovascular system diseases, etc., and can solve problems such as toxic and side effects
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Embodiment 1
[0012] The experiment aimed at the effect of imperatorin on hypertrophic cardiomyocytes after induction. Firstly, cardiomyocyte hypertrophy caused by Ang II was produced, and the primary cardiomyocytes of SD rats were obtained by enzymatic digestion. The experiments used 4-10 generation cells. Digest cardiomyocytes with 0.125% trypsin, count, make single cell suspension, and use 10 per well 4 Cells were seeded in a 24-well culture plate, 1 mL per well, and transferred into 37 °C CO 2 In the incubator, dosing was performed when the cells grew to 80% confluency. Cardiomyocytes were induced with 10, 1 and 0.1 μmol / L Ang II, 1 mL per well. At 37°C, 5% CO 2 and saturated humidity conditions, and at the same time each well was mixed with [ 3 H]-leucine continued to cultivate for 48h. Cardiomyocytes were induced with 10, 1 and 0.1 μmol / L Ang II for 48 hours. It was found that 1 μmol / L Ang II could significantly induce the protein synthesis of cardiomyocytes, and the effect was b...
Embodiment 2
[0014] Example 2: Effect of imperatorin on left heart hypertrophy and myocardial fibrosis in SHR rats
[0015] To test the effect of imperatorin on left heart hypertrophy and myocardial fibrosis in SHR rats, first investigate its effect on hypertrophy index, that is, imperatorin has a dose-dependent down-regulation of left heart hypertrophy index (LVW / BW) effect, and is superior to captopril. Compared with the SHR control group, the left ventricular weight (LVW) of the high-dose imperatorin group decreased significantly (P<0.01). Significant difference (P<0.001); the right ventricular weight (RVW) of each dose group of imperatorin did not decrease due to the decrease of left ventricular weight (LVW), see Table 1. The high-dose imperatorin group even raised the right ventricular index (RVW / BW) to a certain extent, making the heart composition ratio more reasonable.
[0016] Table 1
[0017]
[0018]
[0019] Note: ** P* P<0.05 vs SHR group.
[0020] The effect of the...
Embodiment 3
[0034] To test the relationship between the effect of imperatorin on myocardial hypertrophy and the pathway of NO, the animal model was first established by unilateral renal artery stenosis, that is, the mice were fasted for 12 hours before the operation and had free access to water. The next morning, the mice were anesthetized by intraperitoneal injection of 20% wulai sugar (5mL / Kg). After the mice had no blinking response, they were fixed on the operating table in a supine position, and the left abdomen was skinned. The operation area was about 1×1cm 2 , sterilize the skin with 1% iodine and 75% ethanol, respectively, and make an opening about 0.5 cm below the left rib, 1 cm parallel to the midline of the rib, and the size of the opening is about 1-2 cm, exposing the intra-abdominal organs. Use tweezers to pick up a small piece of sterile gauze, enter from the edge of the left abdominal cavity of the mouse, move the stomach and other organs above the left kidney to the right ...
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