Trithiocyanuric acid dressed gold-size nanoprobe-based colorimetric determination method of mercury ions

A technology of thiocyanate and nano-probes, which is applied in the field of detection and analysis of mercury ion concentration, can solve the problems of poor selectivity, cumbersome sensor operation, and long time consumption, and achieve the effect of simple operation, good specificity, and low cost

Inactive Publication Date: 2012-03-14
EAST CHINA NORMAL UNIV
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  • Abstract
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Problems solved by technology

Currently, DNA (Lee, et al.Angew.Chem., Int.Ed.2007, 46, 4093-4096; Li, et al.Angew.Chem., Int.Ed.2008, 47, 3927-3931; Liu , et al.Chem.Commun.2008, 2242-2244; Wang, et al.Gold Bull.2008, 41, 37-41.) or mercaptopropionic acid (Huang, et al.Chem.Commun.2007, 1215-1217 ) colorimetric sensors based on gold colloidal nanoparticles for recognition elements have been reported, but these sensors have the disadvantages of cumbersome operation, long time-consuming, poor selectivity, etc.

Method used

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  • Trithiocyanuric acid dressed gold-size nanoprobe-based colorimetric determination method of mercury ions
  • Trithiocyanuric acid dressed gold-size nanoprobe-based colorimetric determination method of mercury ions
  • Trithiocyanuric acid dressed gold-size nanoprobe-based colorimetric determination method of mercury ions

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preparation example Construction

[0023] ① Preparation of gold colloidal nanoprobes modified by thiocyanate: refer to the method reported in the literature [K.G.Grabar, R.G.Freeman, M.B.Hommer, M.J.Natan, Anal.Chem.1995, 67, 735] to prepare gold colloidal nanoparticles (13.1 ± 0.5nm); Stir the mixed solution of 150nM thiocyanuric acid and 3nM gold colloidal nanoparticles prepared above at room temperature, incubate for 12 hours, centrifuge and wash twice, then dissolve in boric acid buffer solution with pH=5.0 and stored at 4°C until use. image 3 is the transmission electron microscope image of the prepared gold colloidal nanoparticles.

[0024] ② Add the solution to be tested for mercury ion concentration dropwise into the boric acid buffer solution of the thiocyanate-modified gold colloidal nanoprobe prepared above, shake well; Record the absorbance at 520nm and 600nm respectively, according to A 600 / A 520 To calculate the concentration of mercury ions in the sample. The detection limit of the mercury ...

Embodiment 1

[0025] Embodiment 1: the mensuration of mercury ion in river water water sample

[0026] A mixed solution of 150nM thiocyanuric acid (purchased from sigma) and 3nM gold colloidal nanoparticles (13.1±0.5nm) was stirred at room temperature on a magnetic stirrer, and the ligand exchange reaction was carried out for 12 hours. Excess thiocyanuric acid was removed by centrifugation (10000r / min, 20min), then washed once with three times of distilled water, the centrifugation conditions were the same as above, and finally dispersed in boric acid buffer solution of 10mM pH=5.0 to prepare thiocyanic acid The modified gold colloidal nanoprobes (TCA-AuNPs) were stored at 4°C for future use.

[0027] Using Shanghai Liwa River water as the actual sample, the river water was first decolorized with activated carbon, and then filtered; the river water was filtered through a 0.22 μm membrane, and then centrifuged at 16,000 rpm for 15 minutes, and the supernatant was taken as the water sample to...

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Abstract

The invention discloses a method for simply and quickly measuring mercury ions based on gold-size nanoprobe which is dressed by trithiocyanuric acid, which is characterized in that: trithiocyanuric acid is adopted as a recognition element of the mercury ions, and gold-size nanoparticles which are dressed by the trithiocyaunuric acid as a probe to realize the colorimetric determination of content of mercury ions in a water solution. By utilizing the method for measuring the mercury ions, no pollution is caused on the environment, selectivity on the mercury ions is good, and the interference of a majority of metal ions can be avoided; and the required instrument is simple, the price is low, and the method is suitable for being used in simple and crude fields such as countryside and the like.

Description

technical field [0001] The invention relates to a method for detecting and analyzing the concentration of mercury ions, in particular to a simple and rapid method for measuring mercury ions based on a gold colloidal nano-probe modified with thiocyanic acid. Background technique [0002] Mercury is a highly toxic, non-biodegradable heavy metal ion. It not only causes irreversible pollution of the environment, but also causes a series of diseases related to the nervous system. Therefore, real-time monitoring of mercury ion concentration in drinking water, soil, food and biological samples is extremely important for protecting the environment and public health. At present, the instrument method of detecting mercury ion comprises cold atomic absorption spectrometry, cold atomic fluorescence spectrometry and ICP-MS etc. (Hight, et al.Food Chem.2005,91,557; Roulet, et al.Sci.Total Environ. 2000, 26, 143; Wuillouda, et al. Spectrochim. Acta Part B 2004, 59, 755.). However, these ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 何品刚吴继魁王琪张小燕程圭芳
Owner EAST CHINA NORMAL UNIV
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