Method for preparing honeysuckle flower extract by jointly adopting membrane separation and column chromatography

A honeysuckle extract and column chromatography technology, applied in the separation/purification of carboxylate, organic chemistry and other directions, can solve the problems of loss of alcohol-soluble flavonoids, difficult to guarantee safety, flammable and explosive, etc., and meet the process conditions. The effect of loosening, maintaining biological activity, and high utilization of medicinal materials

Active Publication Date: 2012-03-28
GUANGDONG APOLLO GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

"In the invention patent application, honeysuckle is extracted with water as a solvent, and water extraction will lose a large amount of alcohol-soluble flavonoids; and when purifying the active ingredient, ethyl acetate is added for extraction, but ethyl acetate is an organic solvent and is flammable. Explosive, extreme care is required in laboratory use, and it is even more difficult to guarantee safety in industrial production

Method used

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Embodiment 1

[0038] A method for preparing honeysuckle extract by membrane separation column chromatography, comprising: weighing 20 kg of honeysuckle, in a 300 L extraction tank, adding 160 L of ethanol with a volume percentage concentration of 55% for mixing, and mixing at a temperature of 60 ° C ~70°C and stirring time of 2h, extract by centrifugation to obtain filtrate A and filter residue; repeat the above extraction steps once, add 160L of ethanol with a volume percentage concentration of 55% to the extraction tank, and mix with the filter residue. Extract at a temperature of 60°C to 70°C and a stirring time of 2 hours, and centrifuge to obtain filtrate B; after combining filtrate A and filtrate B, filter through a microporous membrane with a pore size of 0.25 μm to 0.35 μm to obtain filtrate C; Use a 1000L vacuum concentration tank for the filtrate C, and recover the ethanol until it has no alcohol smell at a temperature of 60°C to 70°C, and collect the concentrate at the same time; ...

Embodiment 2

[0044] A method for preparing honeysuckle extract by membrane separation column chromatography, comprising: weighing 23kg of honeysuckle, in a 300 L extraction tank, adding 200 L of ethanol with a volume percentage concentration of 60% for mixing, and mixing at a temperature of 70°C ~80°C and stirring time of 1h, extract by centrifugation to obtain filtrate A and filter residue; repeat the above extraction steps once, add 200L of ethanol with a volume percentage concentration of 60% to the extraction tank, and mix with the filter residue. Extract at a temperature of 70°C to 80°C and a stirring time of 1 hour, and centrifuge to obtain filtrate B; after combining filtrate A and filtrate B, filter through a microporous membrane with a pore size of 0.35 μm to 0.45 μm to obtain filtrate C; Use a 1000L vacuum concentration tank for the filtrate C, and recover the ethanol until it has no alcohol smell at a temperature of 55°C to 65°C, and at the same time collect the concentrate; use ...

Embodiment 3

[0050] A method for preparing honeysuckle extract by membrane separation column chromatography, comprising: weighing 50 kg of honeysuckle, and extracting in two parts, each 25 kg. Add 25kg of honeysuckle to a 300L extraction tank, add 200L of ethanol with a volume percentage concentration of 55% for mixing, extract at a temperature of 65°C to 78°C and a stirring time of 2h, and centrifuge to obtain the filtrate A and filter residue: Repeat the above extraction steps once, mix 200L of ethanol with a volume percentage concentration of 55%, extract at a temperature of 65°C to 78°C, and a stirring time of 2h, and centrifugally filter to obtain filtrate B; combine After filtrate A and filtrate B, filter with a microporous filter membrane with a pore size of 0.45 μm to 0.5 μm to obtain filtrate C; use a 1000L vacuum concentration tank for filtrate C, and recover ethanol until it has no alcohol smell at a temperature of 60°C to 70°C Finally, the concentrated solution was collected at...

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Abstract

A method for preparing a honeysuckle flower extract by jointly adopting membrane separation and column chromatography includes the steps as follows: after preparation of filtrate A and filtrate B, membrane separation, ethanol recycling, column-giving sample-adding and impurity washing, eluting a macroporous resin column by ethanol in volume percentage concentration of 5%-25%, allowing the eluent to pass through a 700-Dalton membrane, conducting pressure reduction and concentration on membrane concentrate in the temperature of 50-80DEG C, and then conducting spray drying, thus obtaining a chlorogenic acid extract; and eluting the macroporous resin column by ethanol in volume percentage concentration of 55%-85%, conducting pressure reduction and concentration on the eluent in the temperature of 50-80DEG C, and then conducting spry drying, thus obtaining a flavone extract. Compared with the prior art, the method adopts the membrane separation technology and the column chromatography technology jointly to prepare the honeysuckle flower extract and can obtain the chlorogenic acid extract and the flavone extract in the honeysuckle flower simultaneously; and the method has such advantages as follows: the utilization rate of medicinal materials is high and the content of the obtained active ingredients is high, so that the subsequent processing and using are facilitated; and the method is simple in technology, low in energy consumption during operation, and convenient for large-scale industrial production and popularization.

Description

technical field [0001] The invention relates to the preparation technology of honeysuckle products, in particular to a method for preparing honeysuckle extract by membrane separation column chromatography. Background technique [0002] Honeysuckle is a commonly used medicinal material with the same origin as food, which has the functions of clearing away heat and detoxifying, cooling and dispelling wind-heat. In recent years, with the occurrence of major epidemics such as SARS and Influenza A H1N1, the anti-epidemic formula recommended by the Ministry of Health uses honeysuckle medicinal materials, making honeysuckle not only have the traditional understanding of heat-clearing and detoxifying, cooling wind-heat effects, but also as a compound compatibility The honeysuckle flower of the Chinese monarch medicine still has the effect of leading the improvement of human immunity. It can be seen that honeysuckle has been more and more widely used in disease prevention and treatm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C69/732C07C67/48C07D311/30C07D311/40
Inventor 陈东岩周春晖杭伟锋邓国江唐志国刘婷婷
Owner GUANGDONG APOLLO GRP
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