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Separation and preparation method for acteoside

A technology of actiglycoside and extract solution, which is applied in the field of separation of natural medicines, can solve the problems that have not yet been reported on actiglycoside, and achieve the effect of large-scale production, high purity and high separation ability

Inactive Publication Date: 2012-03-28
苏州宝泽堂医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no relevant report on the industrial separation and preparation method of actetiside

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The dried aerial part of Arabian Popona was crushed, weighed 0.5kg, added 10 times boiling water to extract 3 times, each time for 1.5h, filtered, combined the extracts, loaded the extracts on D101 macroporous resin column, and washed them with distilled water respectively. elute with 65% ethanol, collect the alcohol eluate, concentrate to remove ethanol, centrifuge through a high-speed centrifuge to obtain the clear liquid, filter through a microfiltration antimony oxide ceramic membrane with a pore size of 0.10 microns, and then pass the filtrate through a hollow fiber membrane with a molecular weight cut-off of 1000 Separate to obtain the permeate, concentrate and dry to obtain the membrane separation component;

[0021] Use reversed phase C18 silica gel bonded stationary phase as chromatographic column packing, 10-55% methanol-water as mobile phase, gradient elution, dissolve membrane separation components with 10ml of anhydrous methanol, inject samples, and monitor ...

Embodiment 2

[0024] The dried aerial part of Arabian Pobana was crushed, weighed 1kg, added 12 times boiling water to extract 3 times, each time for 2 hours, filtered, combined the extracts, and loaded the extracts on D101 macroporous resin column, and used distilled water and 45 % ethanol for elution, collect the ethanol eluent, concentrate to remove ethanol, centrifuge through a high-speed centrifuge to obtain a clear liquid, filter through a microfiltration antimony oxide ceramic membrane with a pore size of 0.10 microns, and then separate the filtrate through a hollow fiber membrane with a molecular weight cut-off of 1000. Obtain the permeate, concentrate and dry to obtain the membrane separation component;

[0025] Use reversed phase C18 silica gel bonded stationary phase as chromatographic column packing, 10-55% methanol-water as mobile phase, gradient elution, dissolve membrane separation components with 10ml of anhydrous methanol, inject samples, and monitor on-line with ultraviolet...

Embodiment 3

[0028] The dried aboveground part of Arabian Popona was crushed, weighed 2kg, added 12 times boiling water to extract 3 times, each time for 1h, filtered, combined the extracts, loaded the extracts on a D101 macroporous resin column, and used distilled water and 50 % ethanol for elution, collect the ethanol eluent, concentrate to remove ethanol, centrifuge through a high-speed centrifuge to obtain a clear liquid, filter through a microfiltration antimony oxide ceramic membrane with a pore size of 0.10 microns, and then separate the filtrate through a hollow fiber membrane with a molecular weight cut-off of 1000. Obtain the permeate, concentrate and dry to obtain the membrane separation component;

[0029] Use reversed-phase C18 silica gel bonded stationary phase as chromatographic column filler, use 10-55% methanol-water as mobile phase, gradient elution, dissolve the membrane separation components with 10ml of anhydrous methanol, inject samples, and monitor on-line with ultrav...

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PUM

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Abstract

The invention relates to separation of natural medicaments, in particular to a separation and preparation method for acteoside. The method comprises the following steps of: smashing the ground part of Arab speedwell; leaching with boiling water for three times, every time for 1-2 hours; combining leaching solutions; performing macroporous resin chromatography; eluting with ethanol water; concentrating the collected liquid and recovering ethanol; feeding the concentrated solution into a high-speed centrifuge for filtering; separating the filtrate with a membrane separator to obtain a component, and separating and preparing with an efficient industrial chromatographic column; gradually eluting with a water-alcohol system; combining eluents which are rich in acteoside; and concentrating and performing freeze drying to obtain acteoside. The method has the advantages of high product purity and stable process, and is particularly suitable for separating and preparing a high-purity acteoside compound from Arab speedwell.

Description

technical field [0001] The invention relates to the separation of natural medicines, in particular to a preparation method for separating actiglycoside from the aerial parts of the arabic sativa by means of membrane separation and preparative high-performance liquid chromatography. Background technique [0002] Arabian mother-in-law ( Veronica persica Poir.) is a herbaceous plant of Scrophulariaceae, which grows in fields and roadsides. It is a common weed in early spring in East China, and it is also a common invasive plant with rich resources. The whole herb of the plant can be used for medicine, and can treat diseases such as rheumatic arthralgia, kidney deficiency and low back pain, and external malaria. The aerial part of araba poba contains active ingredients such as arabica glycosides, actiglycosides, and dulcitol. [0003] Actin is a phenylpropanoid component, soluble in alcoholic organic reagents, soluble in water, and highly polar. Modern pharmacological studies...

Claims

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Application Information

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IPC IPC(8): C07H15/18C07H1/08
Inventor 李法庆刘东锋
Owner 苏州宝泽堂医药科技有限公司
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